The emerging CR6261 antibody could neutralize several subtype influenza virus with high affinity, whose VH domain binds to the HA protein conserved domain. Therefore, it has drawn much attention as a potential broad-spectrum therapeutic antibody against influenza virus. In this study, we constructed the eukaryotic expression vectors pCR6261V(H), pCR6261V(H)-GFP and pCR6261scFv and screened the monoclonal cell lines that could stably express CR6261V(H), CR6261V(H)-GFP and CR6261scFv on the cell membrane. After influenza virus infecting the stable cell lines, the titers of viruses were tested by hemagglutination inhibition test. The result shows that the titers of viruses in CR6261scFv and CR6261V(H)-GFP stable expression cell lines decreased and there was no obvious discrimination between the CR6261V(H) expression cell line and the negative control, suggesting that CR6261V(H) and CR6261scFv expressing on the cell membrane could partly inhibit the virus infection. Though the effective inhibition strategy is undergoing, our research will provide new clues for the breeding of anti influenza transgenic animals.

The association between single nucleotide polymorphisms (SNPs) in the promoter region of MMP-2 and TIMP-2 genes and the risk of epithelial ovarian cancer was investigated. MMP-2 C-1306T, C-735T and TIMP-2 G-418C SNPs were genotyped by polymerase chain reaction-restrictive fragment length polymorphism (PCR-RFLP) analysis in 246 patients with epithelial ovarian cancer and 324 healthy women as control. Results showed no significant difference between the patient and control groups in allele or genotype distributions of MMP-2 C-1306T (P=0.55 and P=0.42). However, the frequencies of the C allele and the C/C genotype of the MMP-2 C-735T were significantly higher in ovarian cancer patients (80.7% and 66.7%) than those in healthy controls (75.5% and 55.9%). Compared with the T/T+C/T genotypes, the C/C genotype significantly increased the risk of ovarian cancer (OR=1.58, 95%CI=1.12-2.23). Stratification analysis showed that subjects carrying C/C genotype were significantly associated with the risk of endometrioid ovarian cancer and with ovarian cancer in subjects that were 50 or older, with odds ratio at 1.69 (95%CI=1.03-2.79) and 1.71 (95%CI=1.14-2.57), respectively. Haplotype analysis showed that the frequencies of four haplotypes (T(-1306)-T(-735), T(-1306)-C(-735), C(-1306)-T(-735) and C(-1306)-C(-735)) of MMP-2 C-1306T and C-735T were not significantly different between the patient and control groups (P=0.24). The allele and genotype frequencies of TIMP-2 G-418C were not significantly different between the patient and control groups (P=0.33 and P=0.47). But TIMP-2 -418G/G genotype was associated with a trend for endometrioid ovarian cancer by stratification analysis according to histological subtypes (OR=1.62, 95%CI=0.94-2.78). Thus, the study suggested that the C/C genotype of the C-735T SNP in the promoter region of MMP-2 gene may be a potential risk factor for epithelial ovarian cancer, but the C-1306T SNP may have no association with the risk of epithelial ovarian cancer. The TIMP-2 G-418C SNP may be associated with the risk of different histological subtypes of epithelial ovarian cancer.

OBJECTIVE To investigate the association of single nucleotide polymorphisms (SNPs) in matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinase-2 (TIMP-2) with the risk of endometriosis and adenomyosis. METHODS Genotypes of MMP-2 and TIMP-2 were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method among 298 endometriosis patients, 180 adenomyosis patients and 324 matched control women. RESULTS No significant difference was found in allele frequencies and genotype distributions of MMP-2 -1306C/T polymorphism between endometriosis patients and control women (P> 0.05). However, there were significant differences in genotype and allele distributions of MMP-2 -1306C/T polymorphism between adenomyosis patients and control women (P< 0.05). Compared with CT+TT genotypes, CC genotype significantly increases the risk of adenomyosis, with an odds ratio of 1.83 (95% CI was 1.13-2.96). No significant difference was shown in allele frequencies and genotype distributions of the MMP-2 -735C/T polymorphism among the three groups (P>0.05). MMP-2 -1306C/T and -735C/T polymorphisms displayed linkage disequilibrium (D'=0.74). There was no significant difference in haplotype distributions of the two MMP-2 SNPs among the three groups ( P> 0.05). No significant difference was found in allele frequencies of TIMP-2 -418G/C polymorphism among the three groups (P> 0.05). However, the frequency of TIMP-2 CC genotype in endometriosis patients (0.7%) was significantly lower than that in the control women (3.7%) (P< 0.05). CONCLUSION The C allele of MMP-2 -1306C/T polymorphism did not modify the risk of developing endometriosis but significantly increase the risk of developing adenomyosis. The MMP-2 -735C/T and TIMP-2 -418G/C polymorphisms were not associated with the risk of developing endometriosis or adenomyosis.

目的通过分析175例经射频消融术证实的房室结折返性心动过速(AVNRT)和房室折返性心动过速(AVRT)患者12导联窦性及心动过速时体表心电图,探讨RP'间期对于判断上述两种类型心动过速的临界参考值以及与心动过速心室率的关系.

目的探讨静注卡托普利降低安氟醚麻醉用于剖腹手术期间过度应激所致循环不良反应的效果及机制.方法择期剖腹手术病人40例,随机分为两组,每组20例.常规插管,安氟醚麻醉.试验组麻醉诱导前10min及切皮后30min分别静注卡托普利0.30～0.35mg/kg及0.15～0.18mg/kg.于麻醉前(基础值)、插管、切皮、探查、缝皮后各1min及术后1h测定SBP、DBP、HR、心率-收缩压乘积(RPP)及血浆去甲肾上腺素(NE)、肾上腺素(E)、一氧化氮(NO)、内皮素(ET)、血栓素B2(TXB2)、6-酮-前列腺素F1α(6-keto-PGF1α)的含量,计算NO/ET、TXB2/PGF1α比值.结果麻醉后,对照组BP、HR、RPP、NE、E及TXB2/PGF1α较其基础值明显升高,NO/ET比值显著降低(P＜0.05或P＜0.01);而试验组NO/ET于插管后1min显著升高(P＜0.05),余指标无明显改变(P＞0.05).与对照组相比,试验组麻醉手术期间BP、HR、RPP、NE及E显著降低,NO/ET比值明显升高,插管和探查后1min的TXB2/PGF1α显著降低(P＜0.05或P＜0.01).结论静注卡托普利降低血浆NE、E的水平,改善NO/ET平衡,降低TXB2/PGF1α比值,可有效地降低安氟醚麻醉剖腹手术期间过度应激的循环不良反应.

普通十二导联心电图对冠心病无症状性心肌缺血及阵发性房性心动过速和室性心动过速等均难以获得,因其发作隐匿,且不定时,易被患者及家属忽视,而且可能是猝死和急性缺血的先兆,常可造成严重后果,所以动态心电图是最有诊断价值的检查方法.

[目的]研究肝癌患者血清中的可溶性细胞间粘附因子-1在肿瘤侵袭和转移中的作用.[方法]采用ELISA方法分别测定原发性肝癌患者和对照组血清中可溶性细胞间粘附因子-1的水平,同时测定AFP含量.[结果]肝癌患者可溶性细胞间粘附因子-1含量显著高于正常人.且与AFP含量呈正线性相关,术后2周浓度降低;Ⅲ和Ⅳ期肝癌患者显著高于I期和Ⅱ期;大肝癌患者显著高于小肝癌患者,有癌栓组、有肝内转移组明显高于无癌栓组和无转移组.[结论]可溶性细胞间粘附因子-1测定在原发性肝癌的诊断和检测术后复发方面有重要价值,联合检测AFP和可溶性细胞间粘附因子-1可提高诊断敏感性.

患者,男性,53岁.主因心前区不适,乏力1周就诊.体检:听诊心律不齐,各瓣膜未闻及病理性杂音,两肺呼吸音清晰,血压130/80 mm Hg(1 mm Hg=0.133 kPa).12导联同步心电图示(图1):心律不齐,最快心率79次/min,最慢心率46次/min,但在缓慢的节律中,仔细观察均发现提前的异位P波,与其前次心动周期的T波重叠在一起形成二联律.最后一个P-QRS波群提前出现,异位P波形态与窦性P波形态不同,P-R间期=0.16秒.心电图诊断:①窦性心律;②房性期前收缩及房性期前收缩未下传构成短阵二联律.

患者,男性,30岁,2002年6月5日16∶00左右工作时突然氨气中毒(由于氨气外溢).吸入氨气后,立刻出现咳嗽、心慌、胸闷、呕吐、四肢无力,17∶00送入我院.查体:神情,口唇发绀.查心电图示:阵发性室上性心动过速(187次/min)及频发室性期前收缩.

患者,女性,75岁,主因胸闷、气短、间断出现1～2年,前后心有间断灼热感、疼痛、憋气、活动时症状更明显,近2～3天病情加重,即来我院就诊.

患者,男,34岁.主诉意识障碍1年,发作10余次,近2个月发作频繁,自觉记忆力减退就诊.发病多与饮酒有关,酒后10余小时出现发凉,四肢无力,嗜睡继之昏睡.

目的:探讨中学教师动态血压(ABP)的变化.方法:采用24小时动态血压仪对100例健康教师进行监测.结果:各年龄组收缩压(SBP)、舒张压(DBP)、平均动脉压(MAP)、心率(HR)相差数值较小,但血压负荷男女之间相差较大,男性SBP 4.26%,DBP 6.54%;女性SBP 1.85%,DBP 4.96%;差别显著(P＜0.01).血压负荷舒张压高于收缩压,差别显著(P＜0.01),同一受试者在不同状态下,动态血压参数有不同程度的变化.结论:推荐中学教师24小时动态血压均值＜130/80mmHg(1mmHg=0.133kPa),日间(6～22时)均值＜135/85mmHg,夜间(22～6时)均值＜125/75mmHg,夜间血压下降率＞8%作为中学教师动态血压正常参照值.

例1,女性,59岁.因发热盗汗1月余,发现颈部肿物20天入院.入院后颈部肿物活检提示恶性淋巴瘤.实验室检查:Hb 100 g/L,RBC 3.41×1012/L,WBC 8.6×109/L,血小板120×109/L.血涂片未找到幼椎细胞.尿本周氏蛋白阴性.