目的 检测阿尔茨海病(Alzheimer's disease,AD)患者血清长链非编码RNA(LncRNA)BC200表达水平,分析其与患者临床特征的关系,探讨其在AD诊断中与血管性痴呆(vascular dementia,VaD)鉴别诊断中的价值.方法 采用实时荧光定量聚合酶链反应对41例AD患者、37例VaD患者、40例对照组患者血清中的LncRNA BC200表达水平进行检测.同时采用ELISA法检测各组患者脑脊液中Aβ1-42蛋白和Tau蛋白水平,分析LncRNA BC200、Aβ1-42和Tau在3组间进行鉴别诊断的价值.应用ROC曲线分析LncRNA BC200、Aβ1-42和Tau诊断AD的敏感度和特异度.结果LncRNA BC200表达水平和AD病程相关(P<0.05),而与性别、年龄和受教育程度均无显著相关(均P>0.05).AD组患者的LncRNA BC200和Tau水平均明显高于VaD组和对照组,Aβ1-42水平显著低于VaD组和对照组(均P<0.01);VaD组患者的LncRNA BC200和Aβ1-42水平与对照组比较,差异无统计学意义(均P>0.05),而Tau水平显著高于对照组(P<0.01).LncRNA BC200、Aβ1-42和Tau三者联合检测的敏感度(88.5％)、特异度(91.3％)和曲线下面积AUCROC(0.965)均比单独检测高,具有较好的诊断价值.结论 AD患者血清中LncRNA BC200表达水平明显升高 , LncRNA BC200可用于AD和VaD的鉴别诊断 ,LncRNA BC200有望成为AD诊断的潜在生物标志物.

Objective To explore the effects of γ,-secretase inhibitor(DAPT) on the airway inflammation,the airway hyperactivity and the imbalance of Thl7/Treg in a mouse model of allergic asthma for providing a theoretical basis for the research and development of anti-asthma activity preparations.Methods Forty-five female C57BL/6 mice were randomly divided into 3 groups:control group,asthma group and DAPT group.The pathological changes of lung tissue were analyzed by hematoxylin-eosin staining.Levels of cytokine and inflammatory mediators in bronchoalveolar lavage fluid(BALF) were measured by enzyme-linked immunosorbent assay.The expression of Foxp3 mRNA in spleen was detected by reverse transcription polymerase chain reaction(RT-PCR).Results The airway inflammation and mucus secretion were alleviated in DAPT group compared with asthma group.The contents of IgE,IL-17,IL-I0 and TGF-β1 in asthma group were (695 + 148) ng/ml,(58 + 6) pg/ml,(148 + 12) pg/ml,(18-+ 3) pg/ml,respectively.The contents of IgE,IL-17,IL-10 and TGF-131 in DAPT group were (486 + 152) ng/ml,(27 ± 6) pg/ml,(193 + 17) pg/ml,(33 ± 5) pg/ml,respectively.Compared with asthma group,the levels of IgE and IL-17 were significantly decreased in DAPT group(P ＜ 0.01),the levels of IL-10,TGF-β1 and the expression of Foxp3 mRNA were significantly increased in DAPT group (P ＜ 0.01).Conclusion The γ-secre tase inhibitor DAPT may efiqciently regulate the imbalance of Th17/Treg to balance a mouse asthmatic model.

Although there have been some studies involving volunteers with inflammatory bowel disease (IBD) who received fecal microbiota transplantation (FMT) with significantly positive results,few existing studies effectively explore the mechanism of FMT from a pharmacological perspective.The purpose of this study was to observe the therapeutic effects and changes of inflammatory cytokines upon FMT through establishment of a rat model of colorectal colitis by using 2,4,6-trinitrobenzene sulfonic acid (TNBS),which may,provide experimental evidences for the treatment of IBD with FMT.A rat model of colorectal colitis was created using TNBS.Four groups of rats were evaluated:control,model (TNBS only),FMT (TNBS and FMT) and SASP (TNBS and salicylazosulfapyridine).The body weight,diarrhea index,and hemafecia scores of the rats in each group were recorded.Changes in colon tissue were studied using histopathology.K +,Na +,albumin (ALB),white blood cell count (WBC),neutrophil percentage (N％),and inflammatory cytokines were also studied.The results showed that,FMT therapy inhibited weight loss,reduced colon shortening,improved intestinal mucosa damage,and caused a significant rise in K + and ALB and a reduction in WBC,N％,and C-reactive proteins(CRP) in the rats.The treatment also inhibited the expression of inflammatory cytokines IL-1 β and IL-17,and promoted an increase in the concentration of anti-inflammatory cytokines IL-10 and IL-10/IL-12.FMT protected the colon mucosa against the development of IBD,promoted the growth of Bifidobacterium,and inhibited the growth of Bacteroides fragilis and Escherichia coli,suggesting a potential use for FMT in the treatment of IBD.

VCS技术是以低频电流分析细胞体积(V),高频电磁探针检测细胞核及核质比特性(C),运用激光扫描细胞,提供细胞的颗粒信息[1],其独特的三维技术不仅用于白细胞分类,而且可以提供多项白细胞VCS相关参数.该参数在多种疾病的辅助诊断具有重要价值,目前在细菌感染[2-3]、疟疾诊断[4]以及急性白血病[5]等领域的研究报道较多.但在过敏性疾病诊断中的报道相对较少,况且其应用的参数主要为中性粒细胞、淋巴细胞、单核细胞的VCS参数,对嗜酸性粒细胞VCS参数的应用价值鲜有研究.为此,对85例过敏性鼻炎患者在确诊之前所测血常规结果中嗜酸性粒细胞VCS参数变化进行回顾性分析,现报道如下.

Objective To investigate the role of the histone deacetylase inhibitor trichostatin A(TSA)in the in vitro differentiation of bone marrow mesenchymal stem cells(BM-MSCs)to hepatocytes in ICR mice.Methods The normal BM-MSCs from ICR mice were isolated and cultured.The surface antigens of mBM-MSCs were analyzed by flow cytometry.Hepatocyte-specific genes a-fetoprotein(AFP)and albumin(ALB)were detected by real-time fluorescence quantitative PCR,the urea secretion by the biochemical analyzer AU5800 and the capacity of glycogen synthesis and storage of hepatocytes by periodic acid-Schiff reaction.The changes of cell proliferation,cell cycle and chromatin structure of mBM-MSCs were detected before and after the TSA treatment.The changes of histon3(H3) and histon4(H4) acetylation were measured in mBM-MSCs by Western blotting.Results Flow cytometry showed that CD44,CD73 and SCA-1 were positive on the surface of mBM-MSCs.Part of the cells expressed CD90,CD105 and STRO-1,and CD11b and CD45 were negative on some cells.After induction for 7 days by TSA,the AFP value was (0.53±0.05) in TSA-treated group,significantly higher than that in TSA-untreated group(P<0.05).ALB and urea secretion began to increase 7 days after the TSA treatment.The ALB expression was (0.52±0.08) and (0.92±0.08),and the urea secretion was (0.23±0.02)mmol/L and (0.36±0.03)mmol/L,respectively,at 14 and 21 days in TSA-treated group,which were significantly elevated as compared with those in TSA-untreated group(P<0.05).Moreover,the glycogen storage of the cells was increased,but AFP levels gradually declined.After the TSA treatment,the greater the concentration of TSA was,the stronger inhibitory effect on mBM-MSCs proliferation.And the ratio of cells at G0/G1 phase was significantly increased.The condensed nuclear heterochromatin became diffuse and loose.Western blotting showed that the levels of H3 and H4 acetylation were significantly increased in mBM-MSCs after 2.0 mmol/L TSA treatment,(P<0.05).Conclusion TSA can increase the differentiation efficacy of mBM-MSCs to hepatocytes by blocking H3 and H4 deacetylation,breaking the equilibrium of histone acetylation,reversing transcription inhibition and therefore promoting the transcription.

Objective To investigate the serum of patients with postpartum thyroiditis(Postpartum thyroiditis,PPT)in the serum of patients with interleukin 6(IL-6)and interferon gamma(IFN-γ)tumor necrosis factor(TNF-α)and anti thyroid peroxidase antibody(TPO-Ab)the clinical significance of detecting content.Methods 34 cases of postpartum thyroiditis were selected in the First Affiliated Hospital of Xi'an Medical University from September 2013 to August 2015.They were treated as the observation group,and selected 34 subjects for health check in the First Affiliated Hospital of Xi'an Medical College at the time as control group.Early in the morning,they all were taken 2 tube of verous blood on an emptystomach and separated serum.Serum IL-6,INF-γ,TNF-α and TPO-Ab of the goups were detected with RATA and antibody sand-wich ELISA,and the differences were compared between the two groups.Results Compared to healthy subjects of 3 kinds of cytokines and antibody levels in the blood of patients with PPT,the data were significantly higher and the difference was statistically significant(t=-98.154,-63.627,-94.090,P<0.05).The content correlated positively with the content of TPO-Ab between the 3 kinds of cytokines in blood of PPT patients(RIL-6=0.852,RTNF-α=0.658.RIFN-γ=0.836).Conclusion Postpartum inflammatory factors and immune factors in the blood of patients with thyroiditis in secretion increased signifi-cantly,the content of TPO-Ab and the 3 factor was positively related to the pathogenesis of PPT,concluded that the 3 kinds of inflammatory factors are involved,so it can be used as basis for diagnosis of patients with PPT,and can provide reference for effective treatment.

目的 探讨自建乙型肝炎病毒核酸(HBV-DNA)实时荧光定量PCR检测系统性能验证的方法和程序,充分了解其检测性能,评估并确认分析性能是否符合预期用途. 方法 参考美国临床实验室标准化委员会(NCCLS)颁布的EP系列文件及中国合格评定国家认可委员会(CNAS)对实验室检测系统评估的要求,对实验室自建的HBV-DNA实时荧光定量PCR检测系统进行精密度、正确度、线性范围、检测下限等性能参数的验证和评价. 结果 HBV-DNA定量检测低值和高值的批内精密度CV分别为3.4％和1.8％,总精密度CV分别为3.8％和1.8％.正确度初次验证有两项不符合要求,经厂家校准后重新验证符合要求.线性回归方程为y =0.997X +0.012,R2=0.999,线性范围为500-108 IU/ml.检测下限为500 IU/ml. 结论 自建HBV-DNA实时荧光定量PCR检测系统的性能符合要求,能够应用于临床检测.

目的 探讨分离、培养的ICR(Institute of Cancer Research,ICR)小鼠骨髓间充质干细胞(mouse bone marrow mesenchymal stem cells,mBM-MSCs)的细胞生物学特性.方法 取6～8周龄ICR小鼠,利用全骨髓反复贴壁和有限稀释培养法分离纯化mBM-MSCs,观察形态特点,测定生长曲线和活力,利用流式细胞仪分析细胞的周期和鉴定表面抗原,诱导其向成骨、软骨及脂肪细胞分化,采用染色法鉴定.结果 新分离的mBM-MSCs多呈小圆形,形态规整.培养传代后,细胞多变为梭形,大小较均匀,形态较一致.随着传代的次数增加,细胞的生长曲线、活力及周期呈现快速发育期、平台期和缓慢期;流式结果细胞CD44、CD73、SCA-1呈阳性反应,部分细胞CD90、CD105、STRO-1呈阳性反应,CD11b和CD45呈阴性反应;诱导分化为成骨细胞后其碱性磷酸酶、茜素红和Von Kossa银染色均呈阳性反应,分化成脂肪细胞后油红O染色呈阳性反应,分化成软骨细胞后阿尔新蓝染色呈阳性反应.结论 ICR小鼠骨髓中分离培养出的MSCs,生物学特点鲜明,适于做进一步研究.

目的 探讨腹水端粒酶活性(telomerasc activity,TA)和癌胚抗原(carcinoembryonic antigen,CEA)检测对鉴别良、恶性腹腔积液的价值. 方法 采用聚合酶链反应酶联免疫吸附测定(PCR-ELISA)法和化学发光微粒子免疫分析技术分别测定65例恶性腹腔积液和52例良性腹腔积液中TA和CEA表达水平. 结果 腹腔积液中TA和CEA的阳性率在恶性腹腔积液组分别为76.9％(50/65)和61.5％(40/65),在良性腹腔积液组分别为7.7％(4/52)和23.1％(12/52),两组比较差异有统计学意义(x2 =62.921,17.925,P＜0.05).TA测定诊断恶性腹腔积液的敏感性为76.9％(50/65),特异性为92.3％(48/52);CEA诊断的敏感性61.5％(40/65),特异性76.9％(40/52),两组比较差异有统计学意义(x2=4.113,5.216,P＜0.05).两者联合检测的敏感性为92.3％(60/65),特异性为96.2％(50/52),联合检测的敏感性显著高于TA和CEA单项检测(x2=4.582,19.231,P＜0.05). 结论 检测腹腔积液TA、CEA表达水平有助于积液性质的良恶性鉴别;联合检测能提高恶性腹腔积液诊断的准确度.

目的：探讨溶血磷脂酸（LPA）、同型半胱氨酸（HCY）、D-二聚体（D-D）对急性冠状动脉综合征（ACS）的预警作用。方法：102例ACS患者分为急性心肌梗死（AMI）组50例和不稳定型心绞痛（UAP）组52例,另选健康体检者53例作为对照组。所有入选者采静脉血进行LPA、HCY、D-D测定;计算单独检测、两两联合检测以及同时检测LPA、HCY和D-D预警ACS的敏感度和特异度,并相互比较。结果：AMI组和UAP组血清LPA、HCY、D-D水平均明显高于对照组（均P〈0.01）;AMI组血清LPA、HCY、D-D水平较UAP组升高（均P〈0.05）;ACS组LPA、HCY、D-D的检测阳性率分别是42.16%、61.76%、46.08%,对照组分别是3.78%、1.89%、1.89%,两组比较有统计学差异（P〈0.01）;单独检测LPA、HCY、D-D灵敏度分别为42.16%、61.76%、46.08%,特异度分别为96.23%、98.11%、98.11%;两两联合检测的灵敏度分别为69.61%、70.59%、59.80%,特异度分别为94.34%、96.23%、96.23%;3个指标联合检测的灵敏度为76.47%,特异度为94.34%。结论：LPA、HCY、D-D水平与ACS的发生、发展有着密切的关系,联合检测三指标可以显著提高ACS的早期诊断率,对ACS的预警作用最强。

目的:探讨溶血磷脂酸(LPA)、同型半胱氨酸(HCY)、D-二聚体(D-D)对急性冠状动脉综合征(ACS)的预警作用.方法:102例ACS患者分为急性心肌梗死(AMI)组50例和不稳定型心绞痛(UAP)组52例,另选健康体检者53例作为对照组.所有入选者采静脉血进行LPA、HCY、D-D测定;计算单独检测、两两联合检测以及同时检测LPA、HCY和D-D预警ACS的敏感度和特异度,并相互比较.结果:AMI组和UAP组血清LPA、HCY、D-D水平均明显高于对照组(均P＜0.01);AMI组血清LPA、HCY、D-D水平较UAP组升高(均P＜ 0.05);ACS组LPA、HCY、D-D的检测阳性率分别是42.16％、61.76％、46.08％,对照组分别是3.78％、1.89％、1.89％,两组比较有统计学差异(P＜0.01);单独检测LPA、HCY、D-D灵敏度分别为42.16％、61.76％、46.08％,特异度分别为96.23％、98.11％、98.11％;两两联合检测的灵敏度分别为69.61％、70.59％、59.80％,特异度分别为94.34％、96.23％、96.23％;3个指标联合检测的灵敏度为76.47％,特异度为94.34％.结论:LPA、HCY、D-D水平与ACS的发生、发展有着密切的关系,联合检测三指标可以显著提高ACS的早期诊断率,对ACS的预警作用最强.

Objective To explore the correlation of D-dimer with coronary artery lesions in patients with acute coronary syndrome (ACS). Methods Sixty patients with ACS were enrolled in this study,including 30 patients with acute myocardial infarction(AMI) and 30 patients with unstable angina pectoris( UAP) . Thirty healthy subjects were chosen as controls. The peripheral venous blood were taken in all patients to detect the level of D-dimer. The lesions of coronary arteries were assessed by Gensini score. The relation of serum D-dimer with Gensini score was analyzed in ACS patients. Results The level of D-dimer was higher in ACS group( inclding AMI and UAP)than that in control group(P<0. 01). Gensini score was significantly higher in AMI group than in UAP group(P<0. 01). The level of D-dimer was positively related with Gensini score in ACS patients(r=0. 586,P<0. 01). Conclusion Level of D-dimer in patients with ACS may be closely related to the atherosclerostic lesions of coronary arteries.

Objective To study the relationship between gene polymorphism of apolipoprotein E (apolipoproteinE,ApoE)of peripheral blood and Mycoplasma pneumoniae infection in children.Methods Collected 236 cases serum of inpatient and outpatient screening in children with Mycoplasma pneumoniae infection and healthy children between March 2011 and March 2014 in the First Affiliated Hospital of Xi’an Medical University and Xi’an Children’s Hospital,at the age of 3~8 years old,divided into two groups:110 cases of control group and 126 cases of Mycoplasma pneumoniae infection in chil-dren.Used multiple allele-specific PCR (multi-AS PCR)to detect gene polymorphism of ApoE in each group.Results ApoE gene was polymorphic and 6 genotypes:3 homozygous (ε2/2,ε3/3,ε4/4)and 3 heterozygote (ε3/2,ε3/4,ε4/2).Theε3/2 had four bands,ε3/3,ε3/4 and 4/2 had three bands,ε2/2 andε4/4 had two bands.ε3/3 of ApoE genotype distribution in two groups was the most common,control group was 66.7%,infection group was 46.4%.Allele frequencies ofε3 and genotype frequencies ofε3/3 inMycoplasmapneumoniae infection of children were lower than those in control group (P<0.05).But allele frequencies ofε4 and genotype frequency ofε4/4 in Mycoplasma pneumoniae infection of children were increased, which were compared with those in control group (P<0.05).Conclusion There were an association between ApoE gene polymorphism and the incidence of Mycoplasma pneumoniae infection in children.Allelesε3 seems to be a protective factor and allelesε4 may contribute to the development of Mycoplasma pneumoniae infection of children.

Objective To investigate the correlations of serum human mammaglobin(hMAM)levels with the early diagnosis of breast cancer and cancer micro-metastasis and their clinical significance.Methods By enzyme-linked immunosorbent assay(ELISA),the serum hMAM levels of 68 patients with breast cancer,40 patients with other cancers (1 0 patients with prostate cancer,1 0 patients with gastric cancer,1 0 patients with ovarian cancer and 1 0 patients with colorectal cancer),35 patients with benign breast disease and 40 healthy females (control group)were determined and analyzed comparatively.The 68 patients with breast cancer were classified according to TNM stage,whether existed the estrogen receptor (ER)expression,axillary lymph node metastasis and menopause.The cut-off value of serum hMAMin patients with breast cancer was determined by receiver operating characteristic (ROC)curve.Results The area under ROC curve of serum hMAM was 0.825,and the reliability in the diagnosis of breast cancer was 82.5% [95%confidence interval (CI):72.6%-92.4%].When the cut-off value for hMAM was 8.43 ng/mL,the sensitivity and specificity were 76.5% and 82.9%,respectively.Serum hMAM levels and positive rate in breast cancer group were significantly higher than those in benign breast disease group,other cancer group and control group (P<0.05 ).The difference among benign breast disease group,other cancer group and control group was not statistically significant (P>0.05).The positive rates of serum hMAM ofⅢ and Ⅳ breast cancer patients (55% and 75%)were significantly higher than those ofⅠand Ⅱpatients (25%and 40%,P<0.05),but hMAMlevels had no difference (P>0.05). The positive rate of serum hMAM in breast cancer patients with axillary lymph node metastasis (88%)was significantly higher than those without metastasis (30%,P<0.05),but hMAMlevels had no difference (P>0.05).Regardless of whether existed ER expression,proto-oncogene C-erb-2 expression and menopause,serum hMAM levels and positive rate of patients with breast cancer had no difference (P>0.05 ).Conclusions The hMAM expression is related with&nbsp;the clinical stage and axillary lymph node metastasis of breast cancer.The determination of serum hMAM level is helpful to improve the early diagnosis of breast cancer and early detection of cancer micro-metastasis.

目的：研究 VCS 技术检测白细胞时无中性粒细胞和嗜酸性粒细胞分类结果的原因。方法采用以 VCS 技术为检测原理的血分析仪器分析血常规标本，无中性粒细胞和嗜酸性粒细胞分类结果的为测试组，有中性粒细胞和嗜酸性粒细胞分类结果的为对照组，分别对以上两组中的中性粒细胞和嗜酸性粒细胞的体积（V）、传导性（C）和光散射（S）这3项参数进行统计。制备血涂片染色后，于显微镜下进行白细胞形态观察并进行分类计数。结果对照组中性粒细胞的 VCS 参数分别为 V：（141．8±6．9）fL、C：145±7．3、S：136±4．8；对照组嗜酸性粒细胞的 VCS 参数分别为 V：（152．9±10．1）fL、C：147±12．0、S：194±7．6。染色后显微镜下观察细胞形态无明显异常；测试组两类细胞的3项 VCS 参数仪器皆未提供，经涂片染色后显微镜观察细胞形态和分类计数后，其嗜酸变的中性粒细胞比例皆大于40％。结论 VCS 技术检测白细胞若出现无中性粒细胞和嗜酸性粒细胞分类结果的标本，应该制备血涂片并进行瑞-吉染色，人工镜检分析其实际分类比例并在报告中予以描述。

目的 探讨Beckman-coulter AC·T 5diff血液分析仪检测新生儿嗜碱性粒细胞计数结果的准确性.方法 收集该院2010年~2011年新生儿患者(年龄＜28天)末梢血标本1 500例(男、女各750例),分别利用Beckman-coulter AC·T 5diff血液分析仪和手工制片法检测标本中嗜碱性粒细胞,并运用统计学方法比较仪器和手工两种方法的结果.结果 实验结果显示,仪器法计数的嗜碱性粒细胞结果为(14.9±5.6)%,手工法为(3.2±1.6)%,差异具有统计学意义(t=2.467,P＜0.05).结论 仪器法分类计数新生儿嗜碱性粒细胞结果偏高,应采用手工分类加以纠正.

目的 研究Beckman-Coulter AC.T 5diff血液分析仪检测白细胞时无中性粒细胞和单核细胞分类结果的原因.方法 对仪器检测白细胞时无中性粒细胞和单核细胞分类结果的标本,涂片染色后显微镜下进行白细胞形态观察并进行分类计数.结果 凡仪器检测无中性粒细胞和单核细胞分类结果的标本,经显微镜观察计数后,其中性杆状核粒细胞比例皆大于15％.结论 对Beckman-Coulter AC.T 5diff血液分析仪检测白细胞时无中性粒细胞和单核细胞分类结果的标本,应认真涂片复检并分类计数中性杆状核粒细胞,为临床提供准确检验结果.

Objective To investigate the infection situation of Mycoplasma pneumonia(Mp) in patients with community-acquired respiratory tract infection and the molecular drug resistance mechanisms of macrolide,and to analyze the relationship between 23SrRNA gene mutation site of isolates resistant to Mp and drug resistance phenotype.Methods A total of 400 throat swab specimens of community-acquired respiratory tract infection were cultured to isolate Mp,the clinical isolates were identified by nested polymerase chain reaction,and the in vitro antibiotic sensitivity test was performed for identifying macrolide-resistant isolates through the minimal inhibitory concentration(MIC).The sequences of macrolide-resistant 23SrRNA gene were detected.The sequences were compared to the corresponding sequences of M129.The relationship between mutation site and drug resistance phenotype was analyzed.Results A total of 50 Mp were isolated from 400 throat swab specimens.Of the 50 isolates,32 isolates were susceptible to macrolide,and 18 isolates were resistant to macrolide.The 18 clinical isolates appeared mutation A2063G,A2064G and A2067G,separately.A2063G showed 14 ring macrolide resistance.A2064G showed 14 and 16 ring macrolide resistances.A2067G showed josamycin resistance.Conclusions Mp to macrolide resistance is serious,and the mutation of 23SrRNA gene is a predominant mechanism that contributes to the macrolide resistance.Through the analysis of 23SrRNA gene mutation site and drug resistance phenotype,the clinical Mp drug resistance situation is obtained.The theoretical guidance for reasonable selection and application of antibiotics is provided.

本文探讨AFP、AFU、IGF-Ⅱ联检对于原发性肝癌(PHC)诊断的临床价值.1 材料和方法 1.1 对象:收集本院2009年6月~2010年8月门诊和住院肝病患者及健康查体者共计90例,分为四组:肝癌31例(男17,女14),年龄(24~79)岁,平均37.5岁;肝硬化29例(男20,女9),年龄(37~70)岁,平均46.1岁;肝炎20例(男15,女5),年龄(27~78)岁,平均35.4岁和正常对照组37例(男22,女15),年龄(23~44)岁.