The purpose is to explore the regulation of apoptosis at different developmental stages in dairy cow mammary gland.Normal cow mammary gland tissues of different developmental stages were sliced in frozen.TUNEL was used to detect the apoptosis of mammary cell.The results showed that the virgin mammary gland developed slowly,little changes in mammary gland,and at this time,no apoptosis was detected.In pregnancy,mammary gland ductal continued to grow,and apoptosis increased.In initial pregnancy 2m,there were a lot of gland alveolus and simultaneously adipocyte apoptosis began to increase,and appearing a secondary apoptosis peak.In lactation,the structure and function of mammary gland were most complete,no changes in mammary gland structure and apoptosis level was low.In involution,gland alveolus collapsed and a lot of cells were apoptosis.In involution 3d,the apoptosis was highest and then decreased.In involution 30d,the mammary gland was recovered and apoptosis decreased.

<正>系统研究乳腺发育过程中乳腺细胞凋亡的周期性规律及其机理,可以更好地了解乳腺发育结构变化的规律,找到可适当延长泌乳期,提高产奶量的生物调控方法。本研究以荷斯坦奶牛为实验材料,应用TUNEL法对奶牛不同发育时期正常乳腺组织(冰冻切片)的细胞凋亡情况进行了系统检测,检测奶牛乳腺组织中的细胞凋亡信号,确定奶牛不同时期乳腺组织的凋亡规律;应用荧光定量RT-PCR及Western blotting技术,检测奶牛不同发育阶段乳腺组织中细胞凋亡相关

The protein expression and localization of leptin and its long form receptor(OB-Rb) were detected by western blottig methord and a confocal laser scanning microscope.The results showed that leptin protein level was higher in virgin,the lowest in lactation.Then OB-Rb was higher in virgin and pregnancy,lower in lactation,and recovered to the original level in involution.