Objective To investigate the effect of polydopamine-modified poly (lactic-co-glycolic acid) (PLGA) and loaded with insulin-like growth factor 1 (IGF-1) on the material performance,and proliferation and differentiation of MC3T3-E1 cells.Methods The PLGA porous microcarriers were prepared by emulsion-solvent evaporation.The microcarriers were surface-modified with polydopamine and loaded with IGF-1.The surface morphology,surface groups and hydrophilicity of PLGA porous microcarriers were determined by scanning electron microscopy,Fourier transform infrared spectroscopy (FTIR) and contact angle tester before and after polydopamine modification.Lysozyme was used as the model protein,and BCA method was used to examine the protein adsorption of the material before and after the modification.PLGA microcarriers (PLGA group),polydopamine-modified PLGA microcarriers (PD-PLGA group) and IGF-l-loaded polydopamine-modified PLGA microcarriers (PD-PLGA/IGF-1 group) were co-cultured with MC3T3-E 1 cells,respectively.The effect of polydopamine-modified and IGF-l-loaded PLGA microcarriers on proliferation,adhesion and osteogenic differentiation of MC3T3-E1 cells were determined by MTT assay,DIPI staining and alkaline phosphatase (ALP) activity assay.Results Scanning electron microscopy showed that the surface of PLGA microcarriers was porous.Infrared spectroscopy analysis showed that polydopamine was successfully adhered to the surface of PLGA microcarriers.The hydrophilicity of PLGA microcarriers modified by polydopamine was greatly improved,and the adsorption capacity of surface protein was increased significantly (both P＜0.05).At 7 d after co-culture with MC3T3-E 1 cells,the cell proliferation and ALP activity in the PD-PLGA group and PD-PLGA/IGF-1 group were significantly higher than those in the PLGA group (all P＜0.05).At 3 d after co-culture with MC3T3-E1 cells,DIPI staining showed that the number of adherent ceils in the PD-PLGA group and PD-PLGA/IGF-1 group was higher than that in the PLGA group.Conclusion After the surface modification with polydopamine,the biological properties of PLGA porous microcarriers can be significantly improved;and loaded with IGF-1,it can significantly promote cell proliferation and osteogenic differentiation.

颈椎病的发病率逐年增高，保守治疗无效的患者多寻求手术治疗。颈椎前路手术因其直接接触椎间盘及后方骨赘对脊髓的压迫，常能达到彻底的减压效果，同时保证椎体良好的骨性融合，维持正常的椎间高度和生理曲度，已经成为治疗颈椎病的最常见手术方式。颈椎前路手术方式分为融合性手术与非融合性手术。融合性手术历来被认为是颈椎前路手术方式的金标准，目前常用类型分为：单纯植骨组，自体髂骨植骨内固定组，钛网钛板内固定组，椎间融合器（ Cage）和椎体间零切迹ZERO-P组。非融合性手术是上世纪末出现的一种新方法，目的是保留手术节段运动功能，对年轻患者适用。本文通过对各种颈前路手术技术的对比分析，阐明不同术式的优缺点。

腰腿痛在临床上十分常见，Golub等〔1，2〕提出韧带结构可能是椎间孔内的异常结构，并推断其在腰腿痛中的作用。随后人们逐渐意识到并肯定了椎间孔韧带在腰腿痛中的作用，但也有越来越多的学者认为椎间孔韧带结构是椎间孔内正常结构。为能更好理解椎间孔韧带在腰腿痛中的作用，本文将结合国内外研究资料，对腰椎间孔韧带相关知识作一综述。

脊柱骨折和脊髓损伤的患者多数需要手术治疗及长期的卧床制动,经常遇到的并发症是下肢的静脉血栓及肺栓塞。这给治疗带来很大的困难。外伤后患者凝血功能常发生变化,血浆D二聚体（D-dimer,D-D）是体内纤维蛋白在凝血因子ⅷ作用下产生的交联纤维蛋白,经血浆纤溶酶降解后终产物,其水平增高反映体内高凝状态和继发纤溶活性增强,它的增高提示血液处于高凝状态和继发性的纤溶性增强[1,2]。本课题组对脊柱骨折患者,合并或

布鲁氏菌性脊柱炎是一种严重的人畜共患传染病，是由布鲁氏杆菌感染间盘及椎体引起的化脓性炎症。以腰椎好发，其次为胸腰段，其病理改变以椎间盘炎症改变为主〔1〕。患者常因关节痛或持续剧烈腰背痛就诊于骨科。近几年在我国东北有逐渐上升的趋势。目前关于布鲁氏杆菌性脊柱炎的诊断和治疗的报道较少。笔者回顾近年文献，对布鲁氏菌脊柱炎的临床表现、影像学表现及治疗作一综述。