OBJECTIVE:Glioblastoma (GBM) is a common type of cancer with high mortality. Epithelial to mesenchymal transition (EMT) plays a vital role in the development of glioblastoma. The aim of this study is to evaluate the role of miR-125a-5p in glioblastoma and in the tumorigenesis of chemotherapeutic drug-resistant cancer stem-like cells in brain glioma.METHODS:The role of miR-125a-5p in the regulation of CSCs, EMT, migration, and invasion in glioblastoma was measured in this study.RESULTS:We showed the roles of miR-125a-5p in the regulation of CSCs, EMT, migration, and invasion in glioblastoma. miR-125a-5p can inhibit the CSCs phenotype and EMT in glioblastoma cells. In addition, its over-expression can significantly regulate CSCs-associated genes and EMT-associated gene expression in glioblastoma cells.CONCLUSIONS:We concluded that miR-125a-5p is one of the key microRNAs regulating CSCs and EMT programs in glioblastoma. The results suggested that miR-125a-5p might be a novel therapy target for glioblastoma.

Objective:To investigate the postoperative complications of cranioplasty using autologous bone for traumatic skull defect.Methods:Between January 2011 and December 2018, a total of 758 consecutive patients with traumatic skull defect underwent cranioplasty using autologous bone at Department of Neurosurgery, Linyi People′s Hospital. Their clinical data were retrospectively analyzed in this study. The outcomes of cranioplasty, postoperative complications and their occurrence time were documented.Results:All 758 patients underwent cranioplasty successfully. One patient (0.13%) died of massive cerebral infarction. One patient (0.13%) developed delayed epidural hematoma on the second day post operation. The follow-up duration of 757 patients were 12-36 months with a median of 14 months. During the follow-up period, 16 cases (2.11%) reported infection in the surgical site resulting in removal of bone flap, among which infection occurred at 8-72 days post operation (median: 21 days) in 13 cases, and the remaining 3 cases reported infection at 4, 11 and 30 months post operation respectively. Seven cases (0.92%) had severe bone resorption. Bone resorption began to appear at 4-6 months after operation, and severe bone resorption occurred about 1 year after operation.Conclusions:The rates of severe complications and surgical failures can be controlled at relatively low levels in cranioplasty using autologous bone for traumatic skull defect, so autologous skull bone is still a suitable choice for cranioplasty. It is necessary to pay attention to the prevention and control of postoperative infection, especially early infection, and bone resorption needs to be followed up.

1 心理护理 老年人心理状态与成人不一样,性情比较怪癖,固执烦躁,对周围事物反应迟钝,易激动,不易合作等.因此,在护理老年病人时,操作前要耐心细致地作解释,不可强制或责难,对患者要象对亲人一样,使他们在住院期间感到温暖,消除心理障碍,自愿接受和配合治疗,以利于疾病的康复.