目的 研究老年痴呆大鼠口服黄芪颗粒后,其海马组织中的基质金属蛋白酶-9与紧密连接蛋白(Occludin)水平的变化.方法 制备老年痴呆大鼠模型,将研究对象分为对照组、模型组、黄芪颗粒组与吡拉西坦组.黄芪颗粒组:口服黄芪颗粒1.4 g/kg·d,时间为28天,西药组:口服吡拉西坦0.43 g/kg·d,时间为28天,对照组与模型组同时口服相同剂量的生理盐水.用水迷宫评估大鼠的学习记忆能力的变化,免疫印迹测量海马组织中的基质金属蛋白酶-9与Occludin的含量.结果 与对照组相比,模型组小鼠表现的行为能力明显减退,基质金属蛋白酶-9的含量(0.565±0.037)增加而Occludin的含量(0.181±0.019)减少(均P＜0.05);口服黄芪颗粒后,其行为能力显著上升,基质金属蛋白酶-9的含量减少(0.341±0.018)而Occludin的含量(0.293±0.017)增加(均P＜0.05).黄芪颗粒组与吡拉西坦组相比差异无统计学意义(P＞0.05).结论 黄芪颗粒能提高老年痴呆大鼠的学习记忆能力,其机制为降低基质金属蛋白酶-9的含量,增加Occludin的含量.

目的 探讨芪归健脑方对阿尔茨海默病(AD)大鼠神经组织中的基质金属蛋白酶-9(MMP-9)、闭合蛋白-5(claudin-5)和咬合蛋白(occludin)含量的影响.方法 制备AD大鼠模型,造模后进行不同的处理.芪归组:每天口服芪归健脑方1 g/kg,吡拉西坦组:每天口服吡拉西坦0.43 g/kg;对照组与AD组:口服同样剂量的无菌生理盐水,疗程均为30天.所有实验对象投入水迷宫检测其行为变化;免疫印迹评估大脑组织中MMP-9、claudin-5和occludin的含量的改变.结果 与对照组比较,AD组大鼠的学习记忆功能明显减弱,MMP-9的水平(0.572±0.031)上升,而claudin-5(0.188±0.021)和occludin(0.192±0.021)的水平降低(均P＜0.05);口服芪归健脑方后其学习记忆功能明显改善,MMP-9的水平(0.338±0.017)下降,而claudin-5(0.295±0.014)和occludin(0.296±0.011)的水平升高(均P＜0.05).芪归组与吡拉西坦组比较,差异无统计学意义(P＞0.05).结论 芪归健脑方可以改善AD大鼠大脑的学习记忆功能,这与其能下调MMP-9的水平,上调claudin-5和occludin水平紧密相关.

目的 探讨补肾活血助孕汤对子宫内膜炎的影响.方法 选取2016年3月-2018年3月于我院接受诊治的子宫内膜炎的患者120例作为研究的主体,随机将其分成研究组和对照组,各60例,并对其采用不同的方案进行治疗.对照组的患者使用常规的方案进行治疗,研究组的患者使用补肾活血助孕汤进行治疗.对研究数据进行统计分析,比较两组的患者临床治疗的效果以及在治疗之后月经的情况.结果 经过分析对比可知,研究组的患者在临床的治疗效果方面相较于对照组的患者明显要好(P＜0.05);并且研究组的患者在治疗之后月经的情况相较于对照组的患者也明显要好(P＜0.05).结论 在子宫内膜炎的患者治疗之中应用补肾活血助孕汤对于提高临床的治疗效果有帮助,对于患者子宫内膜的修复效果较好,在临床上值得广泛推荐使用.

目的 探讨抗菌药物联合妇科千金片治疗子宫内膜炎的临床疗效及其安全性.方法 选取邯郸市中医院2016年4月—2017年12月收治的子宫内膜炎患者102例,随机分为对照组和干预组,每组51例.对照组患者采用抗菌药物治疗,干预组患者在对照组基础上采用妇科千金片治疗,两组患者均以2周为1个疗程.比较两组患者的临床疗效、临床症状(阴道出血、下腹坠痛、下腹胀痛)消失时间,并观察两组患者不良反应发生情况.结果 干预组患者临床疗效优于对照组,阴道出血、下腹坠痛、下腹胀痛消失时间短于对照组(P<0.05).两组患者均未出现严重不良反应.结论 抗菌药物联合妇科千金片治疗子宫内膜炎的临床疗效确切,其能够有效改善患者的临床症状,促进疾病恢复,且安全性高.

[Objective] To explore the effect of angiotensin Ⅱ (Ang Ⅱ) on proliferation and activity of human endometrial epithelium cells (EECs).[Methods] The EECs was isolated and cultured in vitro.There were the control group and Ang Ⅱ groups in the study.EECs in control group were cultivated by solution without serum,and EECs in Ang Ⅱ groups were cultivated by solution with different concentrations of Ang Ⅱ.The method of MTT was used to detect the proliferation of EECs.The contents of Collagen type Ⅰ (Col Ⅰ) and Fibronectin (FN) in the cultured supernatants were measured by enzyme-linked immunosorbent assay (ELISA).The expression levels of α-smooth muscle actin (α-SMA) and Epithelial cadherin (E-cadherin) in cytoplasm of EECs were detected by immunocytochemistry method.[Results] The proliferation rates of EECs in Ang Ⅱ groups significantly increased,and appeared a dose dependent trend.Compared with control group,α-SMA content (71.71±1.47) increased and E-cadherin content (25.32±1.43) decreased in AngⅡ groups,the contents of Col Ⅰ (0.774 7±0.048 0) and FN(0.758 0±0.042 0) both increased,and differences were statistically significant (P＜0.05).[Conclusion] Ang Ⅱ can promote EECs proliferation,and stimulate EECs to change its vitality.

[Objective] To observe and explore the effect of TGF-β1 on proliferation and activity of human endometrial stromal cells (ESCs).[Methods] The ESCs was isolated,cultured and identified in vitro.The experiment groups include:control group and TGF-β1 groups.Control group:ESCs was cultivated by solution without serous.TGF-β1 groups:ESCs was cultivated by solution (without serous) with different concentration of TGF-β1.The method of MTT was used to detect the proliferation of ESCs.The contents of fibronectin (FN) and collagen type Ⅰ (Col Ⅰ) in the cultured supernatants were measured by enzyme-linked immunosorbent assay (ELISA).Western blot was used to detect the expression levels of MMP-9 and TIMP-1.[Results] TGF-β1 improved the proliferation of ESCs,with a dose-dependent effect.The contents of Col Ⅰ (0.736 6±0.042 4) and FN(0.713 1±0.046 8) increased,MMP-9 level decreased and TIMP-1 level increased,aud the differences were statistically significant between control group and TGF-β1 groups (P＜0.05).[Conclusion] TGF-β 1 can promote ESCs proliferation and increase activity of ESCs.

[Objective] To understand the effect of angiotensin Ⅱ (Ang Ⅱ)and angiotensin-(1-7) [Ang-(1-7)] on the proliferation and transdifferentiation of endometrial epithelium cells (EECs).[Methods] The EECs was isolated,cultured and identified in vitro.The EECs was divided into four groups:control group,Ang Ⅱ group,Ang-(1-7) group and Ang Ⅱ +Ang-(l-7)group.The proliferation of EECs in four groups was observed by MTT mothed;the expression level of α-SMA and E-cadherin protein were detected by immunocytochemistry staining method;and the contents of Fibronectin (FN) and collagen type Ⅰ (Col Ⅰ) in the cultured supernatants were detected by ELISA.[Results] At 48 h and 72 h,the number of EECs in Ang Ⅱ group increased statistically;the expression of α-SMA protein in cells increased (77.67 ±1.78),while the expression of E-cadherin decreased (26.56±2.09);the secretion of Col Ⅰ and FN increased (0.832 0±0.029 2,0.752 8±0.071 2),and compared with the control group,the difference was statistically significant (P＜0.05).The number of EECs in Ang-(1-7)+Ang Ⅱ group decreased;the content of α-SMA in cytoplasm decreased (33.58±1.34),while the content of E-cadherin increased (66.86±2.76);the secretion of Col Ⅰ and FN protein decreased (0.635 5±0.026 5,0.557 3±0.038 5),and compared with the Ang-Ⅱ group,the difference was statistically significant(P＜O.05).[Conclusion] Ang-(1-7) could inhibit the proliferation of EECs induced by Ang Ⅱ and its viability.