目的:研究上皮特异性黏附分子(EpCAM)在正常前列腺组织、前列腺癌组织和骨转移性前列腺癌组织中的表达情况,并探讨其表达水平与临床病理特征及预后的关系.方法:结合公共数据库中的前列腺癌资料,分析EpCAM的mRNA和蛋白质表达水平.收集2014-2018年深圳市南山区人民医院及北京大学深圳医院正常前列腺标本18例、原发性前列腺癌71例、骨转移性前列腺癌标本12例.采用免疫组化EnVision法检测EpCAM蛋白在不同组织内的表达,并分析EpCAM的表达水平与前列腺腺癌临床病理特征的关系.结果:TCGA数据库、Human Protein Atlas数据库的分析结果显示,正常前列腺组织中EpCAM的mRNA和蛋白质水平明显低于前列腺癌组织(P＜0.05);EpCAM在不同种族、年龄及Gleason评分组之间前列腺癌组织中表达比较,差异均无统计学意义(P＞0.05);与正常前列腺组织相比,前列腺癌FLI1+亚型和IDH1突变亚型的EpCAM表达未明显增加(P＞0.05);前列腺癌组织内EpCAM的DNA启动子序列甲基化水平低于正常前列腺组织(P＜0.05),但不同年龄组前列腺癌之间的甲基化水平比较,差异均无统计学意义(P＞0.05).免疫组化检测结果显示,前列腺癌组织中EpCAM蛋白表达水平高于正常前列腺组织(P＜0.05),尤其在前列腺癌骨转移组织表达更高(P＜0.05);在不同pT分期及Gleason评分的前列腺癌中EpCAM的蛋白水平比较差异均有统计学意义(P＜0.05),而脉管及神经束侵犯组与其未侵犯组EpCAM蛋白表达水平比较,差异均无统计学意义(P＞0.05).结论:前列腺癌中EpCAM的mRNA及蛋白水平均高于正常前列腺组织,EpCAM的表达水平与前列腺癌分子分型、临床T分期、Gleason评分和及骨转移均密切相关,EpCAM的表达上调可能参与了前列腺癌的发生、进展和骨转移等过程.

Objective To explore the expression and significance of transcription factor SOX 11 in nasopharyngeal carci-noma .Methods By the prospective study method ,26 cases of nasopharyngeal carcinoma organization specimens and 20 cases of nasopharyngitis organization specimens were selected .Expression of SOX11mRNA of the 2 groups were detected by polymerase chain reaction (PCR).Results SOX11mRNA level of nasopharyngeal carcinoma group and nasopharyngitis group were com -pared,the difference had statistical significance (P<0.05);SOX11mRNA level was related to T stage ,clinical stage,lymph node metastasis,pathology differentiation,and was not related to age,sex and course.Conclusion Transcription factor SOX11 may play a direct or indirect role in tumorigenesis ,tumor cell differentiation and cell proliferation process .

Objective To explore the reason of bad-affection of corticosteroids treatment in Nasal polyps during surgery.Methods A retrospective review was conducted in 204 patients with Nasal polyps treated at shenzhen NanSHan hospital from 2003 to 2005. Results 7 cases has stomach upset,4 cases has sleep disorder,5 cases has intractable hiccup, hypertension in 3 cases,hyperlycemia in 2cases.Conclusion Short-term using Corticosteroids has bad-affection,it is important for abiding the rules of using. Corticosteroids and informing patient during the period of treatment.【

Objective To explore the clinical characteristics and the treatment of otomycosis. Methods The clinical data and otoendoscopic figures of 187 cases(257 ears) with otomycosis were retrospectively analyzed. There were lots of humid epidermic piles in the deep part of external auditory canal under otoendoscope. The external auditory canals were sprinkled with miconazole after local cleaning. Results The follow-up period was half to one year. Eighty-five cases healed after one period of treatment, 61 cases recrudesced one or two times, 25 cases recrudesced 3 times or more. In the 86 recrudescent cases(139 ears), there were 48 ears combined with chronic otitis media, 11 ears with stenosis of external auditory canal. Nineteen cases did not keep on treatment. Eighty-two cases had the habit of digging ear. Aspergillus was the most common isolated fungi after culturing. After therapy, external auditory canal was dry and the swelling of skin was alleviated in otoendoscopic findings. Conclusion Otomycosis is easy to be misdiagnosed and mistreated. Getting rid of the habit of excavating ear-canal is the key to preventing from otomycosis. Otoendoscopic examination is beneficial to the diagnosis of otomycosis. Meanwhile curing chronic otitis media, keeping the external auditory canal dry and using antifungal drugs for enough time are also important.

目的探讨扁桃体摘除术术后出血的多种止血处理措施.方法通过对32例术后出血的各种处理措施,总结其有效经验.结果扁桃体术后出血与局部和全身因素及术者操作经验有关.结论防止扁桃体术后出血,应从术前、术中和术后三方面予以高度重视.

目的探讨声带息肉和小结术后复发的相关因素.方法分析术后复发的52例病人临床资料.结果术后复发与多种因素有关.结论术后复发主要与术后用嗓不当和用嗓过度、吸烟和饮酒、息肉或小结钳除不彻底及与变态反应有关.