Aged garlic, obtained by heating raw garlic (Allium sativum) under high temperature and controlled humidity for a period, possesses a wide range of bioactivities, but its role in ulcerative colitis and its mechanism are not fully elucidated. We investigated the bioactive constituents in aged garlic (AG) and explored the effect of oral AG delivery on DSS-induced murine colitis. The results revealed that the aging process up-regulated anti-oxidative, anti-inflammatory and anti-microbial compounds such as dihydrocaffeic acid, 5-acetylsalicylic acid, verticine, S-allyl-L-cysteine and D-fucose. Oral AG obviously alleviated colitis, reducing colon damage and enhancing anti-oxidative and anti-inflammatory effects. Escherichia coli and Streptococcus equinus dramatically were enriched in the colon of mice with colitis that were strongly associated with Parkinson's disease, bacterial invasion of epithelial cells, aerobactin biosynthesis, and heme biosynthesis, but a distinct AG-mediated alteration in the colon due to increasing abundance of Akkermansia muciniphila, Lactobacillus sp. L-YJ, Bifidobacterium breve, Blautia wexlerae, Desulfomicrobium sp. P100A, and Lentilactobacillus hilgardii was observed. Next, we demonstrated that colonic microbiome reconstruction by oral AG significantly increased the production of short-chain fatty acids such as acetic acid, propionic acid, isobutyric acid, and isovaleric acid. This study provides the first data indicating that oral AG ameliorates colonic inflammation in a gut microbiota-dependent manner. Our findings provide novel insights into the AG-mediated remission of colitis and AG as a functional food for modulating the gut microbiota to prevent and treat colitis.

Correction for 'Oral aged garlic (Allium sativum) alleviates ulcerative colitis in mice by improving gut homeostasis' by Deping Han et al., Food Funct., 2024, https://doi.org/10.1039/D4FO03105A.

本研究旨在探索低浓度血清培养条件对猪繁殖与呼吸综合征病毒(PRRSV)NX-1株在宿主细胞Marc-145中增殖的影响.首先,用感染复数(MOI)为0.2的PRRSV NX-1株接种Marc-145细胞1.5h后,分别用含0、1％和2％胎牛血清(FBS)的维持液培养72 h,显微镜下观察细胞形态变化,通过CCK-8法检测细胞活力;采用间接免疫荧光法(IFA)和荧光定量PCR法分别检测病毒感染后病原在细胞中的表达定位及其ORF7基因的表达;最后,用Reed-Muench法检测病毒滴度.结果显示,感染后第72小时,Marc-145细胞出现明显的细胞病变(CPE),2％FBS培养时比1％FBS的CPE更明显;细胞活力检测结果显示,感染后第24和48小时,添加FBS时显著增强细胞的活力,感染后第72小时,添加2％FBS组的细胞活力显著低于其他2组;在添加1％或2％FBS培养时,IFA显示在细胞胞质中抗原阳性信号较强,而在无FBS培养细胞中信号弱.当维持液中添加FBS培养72 h时,病毒ORF7基因在宿主细胞中的表达显著高于无血清组,且2％FBS组的表达量显著较高,其相应病毒液的滴度也显著高于其他2组.上述结果表明,PRRSV NX-1株感染Marc-145细胞后,在含2％血清的培养基中病毒的增殖能力和毒株感染力较强.

呕吐毒素(Deoxynivalenol,DON)是最常见的霉菌毒素之一,对奶牛健康与奶业发展均构成严重威胁.本文聚焦呕吐毒素对奶牛的影响、目前饲料中呕吐毒素污染情况以及脱毒方法进行综述,以期为减少动物生产中呕吐毒素危害提供参考.

畜禽机体对微量铜元素摄入不足或过量,可对生殖系统产生负面影响,制约种猪、蛋品、奶业等产业发展.铜作为动物机体必不可少的微量元素,具有杀菌、抗氧化等功能,在动物生产中常作为药理剂量的饲料添加剂.生殖健康对动物生产至关重要.文章综述微量铜元素对动物繁殖性能的影响以及饲料中铜的含量,以期为动物生产中铜元素的合理使用提供参考.

宠物临床治疗方式多为输液观察治疗,而输液可能会带来冷刺激,引起一些不良反应,给宠物输液加热35～39℃ 来使输进动物体内的药物与体温相当就显得尤为重要,这样有利于药物更好的发挥作用.