A double antibody sandwich ELISA for detection of Trypanosomaevansivariant surface glucoprotein(VSG)antigen was established by using two monoclonal antibodies against Trypanosoma evansi VSG antigen.The result of sensitivity showed when positive serum was diluted to 3200 doubles,its D450nm value was still greater than the critical value of positive and negative,and specificity test result showed that there was no cross reaction with the antigens of Theileria,Toxoplasma gondii,Chlamydiaand Fasciola gigantica.82 clinical sera were detected by this double antibody sandwich ELISA and its positive rate was 9.76%.The experiment results demonstrated that this double antibody sandwich ELISA for detection of Trypanosoma evansiVSG antigen had good detecting sensitivity and specificity,and could be used as an effective method for clinical detecting buffalo Trypanosoma evansi disease.

1发病情况<br>　　2013年3月21日，武鸣县某猪场就猪病来求诊，并送检患病仔猪5头，发病母猪和仔猪的血液样品各4份。该猪场存栏42头母猪，断奶仔猪132头。3月上旬有8头母猪发病，表现为呼吸急促，经用抗菌、退热等药物对症治疗一周后病情没有好转，发病率升高，并且有19头仔猪死亡，妊娠母猪早产、产死胎和弱仔。该猪场之前仅进行过猪瘟、猪伪狂犬病疫苗免疫。