1发病情况<br>　　2013年3月21日，武鸣县某猪场就猪病来求诊，并送检患病仔猪5头，发病母猪和仔猪的血液样品各4份。该猪场存栏42头母猪，断奶仔猪132头。3月上旬有8头母猪发病，表现为呼吸急促，经用抗菌、退热等药物对症治疗一周后病情没有好转，发病率升高，并且有19头仔猪死亡，妊娠母猪早产、产死胎和弱仔。该猪场之前仅进行过猪瘟、猪伪狂犬病疫苗免疫。

本研究对从广西5种不同动物体内分离到的禽Ⅰ型副粘病毒毒株,进行了HN基因的扩增、克隆和序列分析.5个APMV-Ⅰ分离株HN基因的核苷酸序列同源性为94.7％～98.4％,推导氨基酸序列同源性为96.3％～99％,同源性较高；5个毒株的HN基因与国内广东、山东、福建等地的当前流行株同源性较高,但与经典毒株同源性较低；遗传分析表明HN基因相对保守,都源于同一个基因亚群.

对广西猪源Ⅰ型副粘病毒F基因测序分析,为今后开展猪源副粘病毒的相关研究提供参考,也为有效防控副粘病毒感染奠定基础.参考GenBank发表的相关禽副粘病毒Ⅰ型(Avian paramyxovirus serotype Ⅰ,APMV-X)基因组序列,设计了1对特异性引物,用RT-PCR法分别扩增出病毒各F基因片段,并将目的基因片段回收纯化.测定得F基因的序列结果表明,从猪组织中分离获得1株猪源禽Ⅰ型副粘病毒,分离株F基因112～117位裂解位点的氨基酸组成为R-R-Q-R-R-F,与强毒株DQ417113、AF458012的裂解位点一致.同源性分析结果表明,分离株与标准强毒株的核苷酸同源性分别为87.5％和87.4％.猪源禽1型副粘病毒分离株为强毒株,属于基因Ⅰ型是传统型毒株,广西地区禽Ⅰ型副粘病毒宿主范围呈不断扩大趋势.

为了解禽Ⅰ型副黏病毒的跨种传播以及对HN基因进行序列分析,运用RT-PCR方法,扩增马源禽Ⅰ型副黏病毒广西分离株M10株的HN基因,将其克隆至PMD 18-T载体中,进行序列测定,序列分析表明,M10株的HN基因片段长度约为2.0kb,ORF为1716个碱基,编码571个氨基酸;ORF区与参考毒株的核苷酸同源性为80.9％-99.2％,推导的氨基酸同源性为88.6％-98.6％.在同源性比较的基础上,进一步绘制禽Ⅰ型副粘病毒HN基因的系统进化树,分析表明,M10株与参考毒株YG03、NDV-03、FP1等亲缘关系较近.

【Objective】This study was aimed to understand the biological characteristics of horse origin avian paramyxovirus serotype I and provide references for preventing and controlling the paramyxovirus interspecies transmission.【Method】 In Baise of Guangxi,causative agents were isolated from suspicious samples of horse using chicken embryos inoculation and cultured.After serological experiment and pathogenicity identification,F gene of isolates was amplified using RT-PCR and sequenced.【Result】The isolated strain could agglutinate chicken erythrocytes and was inhibited by Newcastle disease virus antiserum.Its titer of HA and HI was 27and 210,respectively.The MDT and ICPI of chicken embryos was 72 h and 1.48,respectively.The isolated strains,which was moderate susceptible,belonged to genotype Ⅶ,sharing 95.9% and 95.1% nucleotide sequence homologies with NDV04-21 and TW-96p,respectively.According to the phylogenetic tree of F gene,the isolates,NDV04-21 and TW-96p belonged to the same branch,which were APMV-I genotype Ⅶ.Amino acid sequence of the isolate fusion(F) protein cleavage site 112-117 was 3'-R-R-Q-K-R-F-5',which was in accordance with standard virulent strain HER33 and F48E9.【Conclusion】The results indicated that APMV-I infected horses.It was reconfirmed that hosts of APMW-1 were developing in Guangxi area.