Objective:To investigate the feasibility of establishing a side-to-side microvascular anastomosis training model by using the femoral artery and vein in rats.Methods:Twenty male SD rats were used in the study. After left-sided femoral arteries and veins were carefully dissected, side-to-side microvascular anastomosis was performed between femoral artery and vein via continuous suturing technique. The diameter of femora artery and vein, the length of the arteriotomy or venotomy, the distance between the corner of the arteriotomy and the temporary clip and suturing time were documented. Patency of those anastomoses was evaluated immediately, 30 min and 7 days after the procedure.Results:Twenty side-to-side microvascular anastomoses between femoral artery and vein were successfully performed in 20 rats, and the operation success rate was 100%. The diameter of femoral artery and vein was 0.68±0.11 mm (0.50-0.80 mm) and 1.21±0.18 mm (1.00-1.50 mm) respectively. The length of the arteriotomy or venotomy was 2.32±0.22 mm (2.00-2.60 mm). The distance between the corner of the arteriotomy and the temporary clip was 1.00-2.50 mm. The total suturing time was 29.2±3.0 min (26-34 min). The patency rate of 100% was achieved immediately, 30 min and 7 days after the procedure.Conclusion:Side-to-side microvascular anastomosis training model between rat femoral artery and vein is a feasible and effective side-to-side microvascular anastomosis training model.

目的 对经典Ono式异位心脏移植模型进行改良,建立SD-Wistar大鼠腹腔异位心脏移植模型,并探讨相关显微外科手术操作技巧.方法 SPF级雄性SD及Wistar大鼠各50只,全身麻醉后取SD大鼠心脏作为供心,在左锁骨下动脉以远5 mm处切断胸主动脉作为供血动脉,将左肺动脉作为供心静脉.受体采用Wistar大鼠,充分游离腹主动脉与下腔静脉,选择左肾动脉远心端约5 mm附近的腹主动脉及下腔静脉作为吻合口部位,将腹主动脉与下腔静脉的吻合口部位置于不同平面.采用连续缝合的方式分别将供心胸主动脉、左肺动脉与受体腹主动脉、下腔静脉进行端侧吻合.结果 通过该方法建立腹腔异位心脏模型,每例SD-Wistar心脏移植模型总手术时间(60±10)min,其中供心切取时间(10±3)min,受体腹主动脉及下腔静脉游离时间(4±2)min,血管吻合(12±5)min.实验动物术中及术后分均无死亡,术后所有供心全部复跳.2例供心在术后第3天出现搏动减弱,于第5天出现供心停跳.其余大鼠术后1周观察仍存在规律心跳.结论 经改良成功建立了SD-Wistar大鼠腹腔异位心脏移植模型.

Objective To study the effect of microRNA-182 (miR-182) targeting Toll-like receptor 4 (TLR4) on microglial inflammatory response. Methods Microglial cells were transfected with miR-182, small interfering TLR4 RNA (siTLR4) and TLR4(ORF) overexpression plasmids using transfection reagent Lipofectamine 2000. Simulating ischemia and hypoxia by OGD treatment. Detection of TLR4 as a direct target of miR-182 by luciferase reporter assay. Detection of mRNA and relative expression of protein by RT-PCR, Western blot and ELISA. Results miR-182 is associated with the expression of TLR4 in microglia under hypoxic conditions, TLR4 is a direct target of miR-182, and miR-182 can inhibit the expression of TLR4, and reduce the release of inflammatory factors (TNF-α, IL-6, IL-1β) from microglia Conclusion miR-182 inhibits hypoxia-induced microglial inflammatory response by targeting inhibition of TLR4 expression. Key words: MicroRNA-182; Toll-like receptor 4; Microglia; Inflammatory response

目的 采用颞下入路开颅电凝法,建立稳定的SD大鼠大脑中动脉脑梗死模型.方法 60只雄性SD大鼠,随机分为实验组和对照组,每组30只.实验组采用右侧颞下入路进行开颅,形成约4 mm×4mm大小骨窗,显露右侧嗅柬至大脑下静脉之间的这一段大脑中动脉,采用双极电凝将大脑中动脉闭塞后切断.对照组仅去除右侧颞部骨质.术后取脑行TTC染色,并对模型大鼠进行神经功能评定.结果 经开颅电凝大鼠大脑中动脉,制备大脑脑梗死模型,成功率达83.3％,模型大鼠神经功能评分均在3～4分,经TTC染色梗死侧大脑半球颜色苍白,脑梗死区范围一致,梗死体积约(29.85±7.43)％;对照组无任何临床症状,无神经功能缺失表现,经TTC染色无梗死灶.结论 采用开颅电凝法闭塞大脑中动脉,可成功制备大鼠脑梗死模型,脑梗死灶范围稳定,死亡率低,模型成功率高.

目的 观察松果菊苷对人脑胶质瘤细胞迁移及侵袭的影响,并探讨其作用机制.方法 体外培养人脑胶质瘤细胞,将细胞分为对照组、松果菊苷组、转化生长因子β(TGF-β)过表达组、TGF-β过表达+松果菊苷组.使用Lipofectamine3000转染细胞,对照组、松果菊苷组转染空载质粒,TGF-β过表达组、TGF-β过表达+松果菊苷组转染TGF-β质粒.转染后24 h向松果菊苷组、TGF-β过表达+松果菊苷组中加入松果菊苷30μmol/L,继续培养24 h.采用细胞划痕实验观察细胞迁移能力,Transwell实验观察细胞侵袭能力,Western blotting法检测细胞TGF-β蛋白表达量.结果 松果菊苷组划痕愈合率较对照组降低,TGF-β过表达组较对照组升高;TGF-β过表达+松果菊苷组较TGF-β过表达组降低,较松果菊苷组升高(P均<0.05).松果菊苷组穿膜细胞数较对照组减少,TGF-β过表达组较对照组增加;TGF-β过表达+松果菊苷组较TGF-β过表达组减少,较松果菊苷组增加(P均<0.05).松果菊苷组细胞TGF-β蛋白表达量较对照组降低;TGF-β过表达组较对照组升高;TGF-β过表达+松果菊苷组较TGF-β过表达组降低,较松果菊苷组升高(P均<0.05).结论 松果菊苷可以通过阻断TGF-β表达来抑制人脑胶质瘤细胞的迁移和侵袭能力.