目的 研究巨噬细胞迁移抑制因子(MIF)及炎症因子在昼夜节律改变小鼠心脏缺血再灌注损伤中的作用.方法 36只小鼠在12h/12h昼夜交替和持续黑暗环境下饲养2周,分为Normal组、Normal缺血再灌注组、Disrupted组、Disrupted缺血再灌注组,每组9只,4周后完成造模,Normal组和Disrupted组行心超检测、心肌病理检查、心肌MIF、IL-6、TNF-α、IL-8检测.另对Normal缺血再灌注组及Disrupted缺血再灌注组行心脏缺血再灌注处理,处理后分别检测心肌IL-6、TNF-α、IL-8等炎症因子表达,心肌组织局部炎症浸润程度及超声心动图.结果 Disrupted组小鼠心肌MIF表达较Normal组显著下降(P＜0.05).Disrupted缺血再灌注组和Normal缺血再灌注组相比,心脏IL-6、TNF-α、IL-8表达显著增高,病理检查示心肌局部炎症浸润增加,心超示心功能受损加重(均P＜ 0.05).结论 改变小鼠昼夜节律致心肌MIF蛋白表达下降,心肌缺血再灌注后炎症因子IL-6、TNF-α、IL-8表达增加,局部炎症反应加重,心功能恶化.

在病理性心脏肥厚的情况下,血管生成不足导致供氧不足,从而导致低氧诱导因子( hypoxia-inducible factor , HIF)上调,而HIF则可以引起血管内皮细胞生长因子的增加. 这种适应性反应延缓了心脏从病理性肥大到心力衰竭的发展过程[1]. 而在糖尿病心肌中血管内皮细胞生长因子及其受体的表达减少[ 2].

AIMS:Benzo[a]pyrene (BaP), a prominent component of tobacco, has been revealed to induce damage to endothelial progenitor cells (EPCs). Astragaloside IV (AS-IV) is widely used for the treatment of cardiovascular diseases in China. In this study, we evaluated the effects of AS-IV on the function of human EPCs after BaP exposure and explored the underlying mechanism.MATERIALS AND METHODS:Human umbilical cord blood mononuclear cells were isolated using density gradient centrifugation. Cells of the 4th passage were randomly divided into 6 groups. EPCs of experimental groups were pre-treated with different concentrations (2, 10 and 50 μg/mL) of AS-IV for 2h before exposure to BaP (20 μM) for 24h. The proliferation, migration, and adhesion of the treated EPCs were evaluated using a cell counting kit-8, Transwell assay and adhesion assay respectively. Interleukin-1β, tumor necrosis factor-α, malondialdehyde and SOD contents in the supernatant were evaluated. The expression of RAGE protein was measured by Western blotting.KEY FINDINGS:The results demonstrated that AS-IV pre-treatment significantly improved BaP-induced dysfunction of EPCs in terms of proliferation, migration and adhesion. Furthermore, AS-IV reduced the production of reactive oxygen species, malondialdehyde, interleukin-1β and tumor necrosis factor-α of the BaP-treated EPCs. Finally AS-IV pre-treated EPCs showed an increased SOD activity and decreased RAGE protein expression.SIGNIFICANCE:AS-IV is able to prevent BaP-mediated EPC dysfunction by at least inhibiting oxidative stress through the RAGE pathway.

Objective:Explore the effects of Jijitang on the treatment of acute myocardial infarction underwent coronary intervention(PCI) after clinical treatment.Methods:Randomized 104 patients diagnosed with acute myocardial infarction underwent the PCI surgery patients were divided into two groups,the control group(52 cases) after the PCI were given western medicine treatment,the experimental group(52 patients) in the treatment of the control group on the basis were given Ji ji tang.Results:The experimental group after treatment,the cardiac function and plasma inflammatory factors were better than the control group(P0.05),patient outcomes of the two groups were 100%,liver and kidney functions and blood showed no abnormalities,the difference was not statistically significant(P0.05).Conclusion:Patients with acute myocardial infarction after PCI were given Ji ji tang,can significantly improve cardiac function,reduce the level of inflammatory factors improve patients' quality of life and worthy of the application and popularization.

OBJECTIVE To explore the relationship between genetic polymorphisms of CACNA1C that encoded the a1c subunit of the L-type calcium channel and the efficacy of calcium channel blocker (CCB, Nifedipine extended release tablet/20 mg/d) in essential hypertension (EH) patients of Han Chinese in Wenzhou. METHODS For the enrolled 103 EH patients, Multiplex Polymerase Chain Reaction (Multi-PCR) and matrix assisted laser desorption ionization time of flight MS (MLDI-TOF MS) were performed to detect their genotypes (rs216008, rs1051375, rs2299661, rs10848683, rs215976), blood pressure (BP) after CCB monotherapy was compared among patients with different genotypes. RESULTS (1) Blood pressure was significantly reduced in all patients post CCB (P < 0.05 vs. pre-CCB). (2) Diastolic blood pressure reduction was more significant in subjects with rs2299661 C/C genotype (wild genotype) than in subjects with rs2299661C/G and rs2299661G/G genotype (mutational genotype) [(12.46 ± 7.91) mm Hg (1 mm Hg = 0.133 kPa) vs. (7.22 ± 8.01) mm Hg and (5.93 ± 9.77) mm Hg, P < 0.05]. (3) Systolic blood pressure reduction was more significant in subjects with rs216008 C/C genotype (wild genotype) than in subjects with rs216008 C/T genotype (mutational genotype) [(20.60 ± 12.35) mm Hg vs. (13.62 ± 10.21) mm Hg, P < 0.05]. (4) Blood pressure reduction was similar between subjects with genotype of rs1051375, rs10848683 and rs215976. CONCLUSION EH patients with wild genotype of rs2299661 and rs216008 in CACNA1C are more likely to be responders of CCB monotherapy.

Objective To investigate the association between 6 single nucleotide polymorphisms(SNP) of L-calcium channel auxiliary subunit β2 gene(CACNB2) and the efficacy of calcium channel blockers(CCB) in the treatment of essential hypertension.Methods A total of 104 EH patients were given 20mg CCB(extended release nifedipine tablets) monotherapy everyday for six weeks.Six SNPs in the CACNB2 determined by multiplex polymerase chain reaction(multi-PCR) and matrix assisted laser desorption ionization time of flight MS(MLD1-TOF MS) to analyze the association of the CCB-efficacy for hypertension with different SNPs and their genotypes.Results The AA genotype of rs11014166 was more efficient than AT and AA genotype(P<0.05).Efficacy of genotypes of other five SNPs was not different each other(P>0.05).Conclusion The rs11014166 polymorphism of CACNB2 was associated with curative effect of L-calcium channel blockers,which was more effective in patients with AA genotype EH.

Objective:To know the difference of electrocardiogram and echocardiography in patients with acute pulmonary embolism(APE)and chronic pulmonary embolism(CPE).Methods:All of the 53 in-hospital patients with pulmonary embolism from 2001.01 to 2007.03 were divided into two groups:Acute pulmonary embolism group(24 cases)and chronic pulmonary embolism(29 cases).The change of electrocardiogram and echocardiography(include:the size of four chambers,the pressure of the pulmonary artery etc.)were retrospectively analyzed and compared between two groups.Results:There were abnormal changes of electrocardiogram in absolute majority of patients.In APE group,more patients had SⅠ、QⅢ manifestation(P0.05 all);however,more patients had the manifestation of atrial fibrillation(P0.01),RⅥ mV increased(P0.05),the patient number of RⅥ1.0 mV increased(P0.05)in CPE group.Echocardiogram appearance:①Elevation of the pressure of the pulmonary artery by different degree;②Dilation of the right atrium and ventricle by different degree;Dilation of the right atrium and ventricle worsen in CPE group(P0.01,0.05).Conclusion:The manifestation of electrocardiogram and echocardiography,which place a diagnostic role,is different in acute and chronic pulmonary embolism.

目的了解急性冠状动脉综合征患者炎性指标pentraxin-3的变化,并了解其与冠状动脉病变之间的关系。方法回顾性研究从2003年7月到2005年9月期间85例急性冠状动脉综合征患者及25例健康体检者,检测其血浆pentraxin-3水平,并进行比较。所有急性冠状动脉综合征患者行选择性冠状动脉造影,了解pentraxin-3与冠状动脉病变之间的关系。结果急性冠状动脉综合征患者血浆pentraxin-3水平,明显高于健康体检者（P＜0.01）,且急性心肌梗死组高于不稳定性心绞痛组（P＜0.01）。A型病变、B型病变、C型病变三种病变类型患者血浆pen-traxin-3差异无显著性。结论作为炎性指标,pentraxin-3与冠心病发生急性冠状动脉综合征密切相关,但与冠状动脉病变类型无明确相关。

Objective:To evaluate the role of echocardiography in patients with acute pulmonary embolism and chronic pulmonary embolism.Methods:All of the43in-hospital patients with pulmonary embolism divided into two groups: acute pulmonary embolism and chronic ones.Their manifestation of echocardiography was retrospectively and analyzed (include: the size of four chambers,the pressure of the pulmonary artery,the thrombosis in the main trunk and its bifurcation of the pulmonary artery).Results:There were dilation of the right atrium and ventricle in two groups,but the dilation of the right atrium and ventriclewas worse in chronic group(P0.05~0.01).Conclusion:The manifestation of echocardiography contributes to detecting the acute or chronic pulmonary embolism.