In the sarcoma S180 ascitic mice, the effects of moxa-cone moxibustion at Guanyuan (CV 4) on erythrocytic immunity and its regulative function were investigated. The results indicated that moxibustion at Guanyuan (CV 4) could significantly increase the decreased erythrocytic C3b receptor rosette forming rate (RBC-C3bRR), lower the raised erythrocytic immunocomplex rosette forming rate (RBC-ICR, P < 0.05 or P < 0.01), increase the decreased activity of erythrocytic immuno-accelerative factor, and reduce the increased activity of erythrocytic immunosuppressive factor (P < 0.05) in the tumor-bearing mice. This suggests that moxibustion at Guanyuan (CV 4) can strengthen erythrocytic immunity and promote its regulative function.

Objective To study the immunoprotective property of the 39kD hydrophobic outer membrane protein of Leptospira interrogans (OmpL39) to guinea pigs. For this end, the immunogenic function of GP red cells during immuno responses has to be assayed. This is aiming at providing evidences for preparing genetic engineering vaccine against leptospirosis. Methods In a randomized control trial, 36 guinea pigs were divided into 4 groups and inoculated separately with OmpL39, whole leptospiral cell vaccine (WLCV) , other proteins of Leptospira and normal saline (negative control). Their immunoprotective properties and the red cell immunity function were tested. The results were analyzed by used of the χ 2 and t test. Results The results showed that all the guinea pigs inoculated with OmpL39 and WLCV still survived after challenge, but all the control guinea pigs died. The immunoprotective effectiveness was 100% for OmpL39 and MLCV groups. OmpL39 levels were similar to that of WLCV and higher than control group ( P 0.05). The OmpL39 could produce 100% immunoprotective effectiveness and has higher MAT levels. The red cell immunity property of the guinea pigs changed during immuno response and the RBC C3b RR and the RBC ICR increased. The RFER increased, the RFIR decreased. RFER/RFIR was remarkably higher than that of control ( P 0.05). Conclusions The OmpL39 could increase the red cell immunity function and could be used as important immunoprotective antigen to develop the genetic engineering vaccine.