选用39KDa的钩端螺旋体外膜蛋白(OmpL39)免疫豚鼠并施予艾灸,研究艾灸对OmpL39免疫保护力的影响.结果表明:灸加OmpL39与单用OmpL39都有合格的免疫力,但前者的特异性抗体效价比后者高,提示艾灸通过扶助正气对OmpL39的免疫保护力有促进作用.

Objective To study the immunoprotective property of the 39kD hydrophobic outer membrane protein of Leptospira interrogans (OmpL39) to guinea pigs. For this end, the immunogenic function of GP red cells during immuno responses has to be assayed. This is aiming at providing evidences for preparing genetic engineering vaccine against leptospirosis. Methods In a randomized control trial, 36 guinea pigs were divided into 4 groups and inoculated separately with OmpL39, whole leptospiral cell vaccine (WLCV) , other proteins of Leptospira and normal saline (negative control). Their immunoprotective properties and the red cell immunity function were tested. The results were analyzed by used of the χ 2 and t test. Results The results showed that all the guinea pigs inoculated with OmpL39 and WLCV still survived after challenge, but all the control guinea pigs died. The immunoprotective effectiveness was 100% for OmpL39 and MLCV groups. OmpL39 levels were similar to that of WLCV and higher than control group ( P 0.05). The OmpL39 could produce 100% immunoprotective effectiveness and has higher MAT levels. The red cell immunity property of the guinea pigs changed during immuno response and the RBC C3b RR and the RBC ICR increased. The RFER increased, the RFIR decreased. RFER/RFIR was remarkably higher than that of control ( P 0.05). Conclusions The OmpL39 could increase the red cell immunity function and could be used as important immunoprotective antigen to develop the genetic engineering vaccine.

本文阐述了经络系统和神经—内分泌—免疫网络的联系，对经络—神经—内分泌—免疫网络的结构体系进行了具体的分析，从经络—神经—内分泌—免疫网络调节的角度对针灸调节免疫的机理进行了深入的研究，为针灸免疫学基本理论的建立奠定了基础