目的 探究维甲酸相关孤儿核受体-γ(RORγ)基因对人结肠癌细胞增殖、转移能力的影响.方法 通过构建RORγ敲减细胞系,采用RT-qPCR及Western blot检测敲减效率;MTT、克隆形成、Transwell和划痕实验检测细胞增殖与转移能力;采用Western blot检测上皮间质转化(EMT)相关蛋白的表达情况;通过TIMER 2.0数据库分析RORγ基因的表达与肿瘤微环境中免疫细胞浸润的关系.结果 RORγ基因的敲减增强了结肠癌细胞的活力(F值=157.40,P<0.01)、克隆形成能力(F=61.35,P<0.01)、迁移能力(F=13.00,P<0.01)、侵袭能力(F=21.26,P<0.01)和划痕愈合能力(F=877.2,P<0.01),抑制了上皮型钙黏附蛋白(E-Cadherin)的表达,并促进了波形蛋白(vimentin)和神经型钙黏附蛋白(N-Cadherin)的表达;TIMER 2.0数据库分析显示结肠腺癌(COAD)组织中RORγ的表达水平与多种免疫细胞浸润相关.结论 RORγ表达的下调促进结肠癌细胞的增殖及转移.

目的:探讨转录因子Snail对胃癌MKN-28细胞的影响.方法:取胃癌MNK-28细胞株的Snail基因进行沉默研究,以顺铂对MNK-28细胞进行处理,并选用CCK-8法不同刺激因素组细胞的增殖情况进行检测,同时选用透射电镜对各组细胞凋亡情况进行观察,而各组细胞侵袭能力则以Transwell小室进行观察.结果:(1)细胞增殖情况分析,顺铂处理及慢病毒沉默Snail基因均可见细胞增殖抑制现象,而shRNA+Snail+顺铂组细胞增殖抑制率显著高于其他组(p<0.05);(2)细胞凋亡情况,与单纯MKN-28细胞组比较,不同刺激因素组细胞凋亡程度均显著升高(p<0.05);(3)细胞侵袭实验分析,shRNA+Snail+顺铂组细胞迁出的细胞数较其他组明显降低(p<0.05).结论:转录因子Snail可对胃癌MKN-28细胞的增殖情况产生影响,沉默Snail基因有利于加速细胞凋亡,提高化疗药敏感性,可在临床上推广.

目的 观察黄芪糖蛋白(HQGP)干预肺纤维化小鼠的病理学改变及并分析机制.方法 将60只健康ICR小鼠随机分为对照组、模型组及治疗组,每组20只.采用鼻腔滴入博莱霉素建立小鼠肺纤维化模型,造模次日起治疗组每天腹腔注射HQGP,模型组及对照组腹腔注射等体积的生理盐水,连续14 d;造模后第7、14、28天取材,取肺组织进行HE、Masson染色,观察肺泡炎及纤维化程度;免疫组化法检测肺组织转化生长因子(TGF)-β1表达.结果 与模型组比较,第7、14、28天治疗组肺泡炎、肺纤维化程度明显减轻;肺组织TGF-β1蛋白表达明显降低(P<0.05).结论 HQGP抑制小鼠肺纤维化的发展,其作用机制可能与降低肺组织中TGF-β1表达有关.

小米，是养生达人的首选食品，生病后，产后以及体质虚弱者都可以服用，可以滋阴补血。一年四季均可服用。小米熬成的小米粥素有“代参汤”之美称。原北京朝阳医院副院长沈雁英介绍，小米不仅有养胃的功能，还能助眠安神，对缓解压力、紧张很有效。所以晚上睡不着觉，不妨喝点小米粥。

Objective To investigate wheat maxibastion of Guanyuan point on immune function and antitumor effect in mice with tumor.Method Group experiment and statistical analysis were conducted.Results were analyzed comparatively.Result Wheat maxibastion of Guanyuan point could reduce ATEP(associated tumor erythrocyte rate) and direct tumor erythrocyte rate(DTER) and raise excitotumorous erythrocyte rate(ETER)(P< 0.05,P< 0.01) and stimulate the reduced activity of NK cells and IL 2 significantly(P< 0.01) in mice with tumor.Conclusion Wheat maxibastion of Guanyuan point can increase the adhesion ability to tumor cells of RBC,and increase the activity of NK cell and IL 2.

In the sarcoma S180 ascitic mice, the effects of moxa-cone moxibustion at Guanyuan (CV 4) on erythrocytic immunity and its regulative function were investigated. The results indicated that moxibustion at Guanyuan (CV 4) could significantly increase the decreased erythrocytic C3b receptor rosette forming rate (RBC-C3bRR), lower the raised erythrocytic immunocomplex rosette forming rate (RBC-ICR, P < 0.05 or P < 0.01), increase the decreased activity of erythrocytic immuno-accelerative factor, and reduce the increased activity of erythrocytic immunosuppressive factor (P < 0.05) in the tumor-bearing mice. This suggests that moxibustion at Guanyuan (CV 4) can strengthen erythrocytic immunity and promote its regulative function.

自拟益气活血汤(黄芪、党参、益母草、丹参等)治疗冠心病40例,总有效率95%,提示本方有益气活血,行气化瘀的功效,是治疗气虚血瘀型的有效方法.