Objective:To investigate the profiling of NURR1-overexpressed LNCaP cells by gene chip analysis.Methods:NURR1 was ectopic expressed in LNCaP cells by lentivirus infection, validated by immunocytochemical staining. The profiling of mRNA and lncRNA was compared by gene chips between NURR1-overexpressed LNCaP cells and vector-transfected control. KEEG and GO analysis were performed to study the potential functional role and signaling pathways involved.Results:A total of 515 genes were differentially expressed in NURR1-overexpressed LNCaP cells as compared to its vector-transfected control, including 238 mRNA upregulated, 195 mRNA down regulated, 54 lncRNA upregulated and 28 lncRNA downregulated. The differentially expressed mRNA and lncRNA were mainly involved in molecular functions, among which the retinal binding function, iron ion binding capacity, nerve axon regulation and redox process, and the cytological composition of the extracellular region exhibited the most significance. The interaction between cytokines and cytokine receptors, as well as stem cell pluripotency related signaling pathways were changed significantly.Conclusion:The mRNA and lncRNA profiling has been significantly changed by the ectopic expression of NURR1, which might play critical role in the development progression of prostate cancer.

Background and purpose: Nuclear receptor hepatocyte nuclear factor 4α (HNF4α) is a critical transcriptional factor, however, the functional and regulatory role of HNF4α in prostate cancer is not clear. The aim of this study was to investigate the mechanism of HNF4α involved in the development of castration-resistant prostate cancer. Methods: Oncomine analysis was used to characterize the expression level of HNF4α in clinical samples of prostate cancer. cBioPortal analysis was used to study the alteration of HNF4α in different subtypes of prostate cancer. The overall survival of prostate cancer patients with altered and unaltered HNF4α was also compared. The plenti-HNF4α vector was constructed and transfected into HEK-293 cells for lentivirus package. HNF4α was ectopically expressed in LNCaP cells, and its effect on androgen receptor (AR) expression level was analyzed by real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR) and Western blot. Luciferase reporter assay was performed to study the potential binding effect of HNF4α on AR promoter. Results: HNF4α was upregulated in clinical samples of prostate cancer, and the proportion of abnormal changes in castration-resistant prostate cancer and neuroendocrine prostate cancer samples was significantly increased, which was negatively correlated with the overall survival rate of patients. The exogenous upregulation of HNF4α significantly promoted the expression of AR, and was positively correlated with the expression of the luciferase reporter gene. Conclusion: HNF4α is upregulated in prostate cancer and likely promotes AR expression by directly targeting the AR promoter. HNF4α may serve as a new therapeutic target in prostate cancer.

Objective To explore the expression file and role of orphan nuclear receptor NURR1 in prostate cancer.Methods Oncomine datasets were analyzed to study the expression of NURR1 in clinical prostate cancer samples.Real-time PCR was used to assay the expression pattern of NURR1 in prostatospheroids and VCaP-CRPC xenograft models.The pLentiNURR1 plasmid was generated and overexpressed in DU145 cells.The prostate cancer stem cell markers and spheroids formation capacity were determined with Real-time PCR and sphere formation experiment.Results NURR1 was significantly upregulated in prostate cancer samples,prostatospheroids and VCaP-CRPC xenograft models.Overexpressed NURR1 in DU145 cells could increase expression of prostate cancer stem cell markers and enhance the sphere formation capacity.Conclusion The orphan nuclear receptor NURR1 may serve as an important marker and therapeutic target in prostate cancer.

目的 评价比卡鲁胺联合戈舍瑞林间断性治疗对晚期前列腺癌的治疗效果.方法 选择2014年6月至2016年1月在我院确诊并治疗的106例晚期前列腺癌患者作为研究对象,随机分为试验组(53例)和对照组(53例),试验组患者应用比卡鲁胺联合戈舍瑞林进行间断性治疗,对照组持续性给药,两组用法用量相同.随访收集并比较两组患者的前列腺特异抗原(PSA)、游离前列腺特异抗原(f-PSA)、排尿梗阻症、骨转移情况及不良反应发生情况.结果 治疗3、6、12个月后,两组的血清PSA、f-PSA水平降低(P＜0.05),排尿梗阻症、骨转移例数减少(P＜0.05),但两组上述指标比较差异无统计学意义(P＞0.05);试验组性功能障碍(39.6％vs.64.2％,P=0.011)及潮热(13.2％ vs.30.2％,P=0.034)的发生率低于对照组.结论 比卡鲁胺联合戈舍瑞林间断性治疗对晚期前列腺癌的治疗效果与持续性治疗相当,且安全性较高.

目的:观察比较免打结后腹腔镜肾部分切除术与开放肾部分切除术治疗小肾癌的效果.方法:选择小肾癌80例,随机分为观察组和对照组各40例.观察组采用免打结后腹腔镜肾部分切除术,对照组采用开放肾部分切除术,比较两组手术时间、热缺血时间、手术出血量、术后留置引流管时间、术后下床活动时间、引流液量、术后镇痛药使用比例、住院时间、术后并发症发生情况等.结果:观察组手术时间、术后留置引流管时间、术后下床活动时间、引流液量和术后住院时间显著或非常显著少(早)于对照组(P＜0.05,P＜0.01),热缺血时间和手术出血量两组比较,差异不显著(P＞0.05).观察组术后使用镇痛药7例,非常显著少于对照组的26例(P＜0.01).术后并发症发生率两组比较,差异不显著(P＞0.05).结论:免打结后腹腔镜肾部分切除术治疗小肾癌效果优于开放肾部分切除术.

目的 研究microRNA-34a (miR-34a)对膀胱肿瘤细胞株5637细胞迁移、侵袭和增殖的影响.方法 在5637细胞株中过表达miR-34a,运用小室迁移实验和小室侵袭实验检测细胞迁移力和侵袭力;运用血球计数板法和新型四唑氮盐(MTS)法检测细胞增殖力.结果 过表达miR-34a后,5637细胞迁移力和侵袭力均下降(P＜0.01),细胞增殖受抑(P＜0.01).结论 过表达miR-34能抑制膀胱肿瘤细胞株5637的迁移、侵袭和增殖.