This paper described a robust and sensitive HPLC method for determination of glyphosate on vegetable material after pre-derivatisation with 9-fluorenylmethyl chloroformate(FMOC-Cl) using coupled polymeric amino columns. New findings about optimization of the derivatisation reaction and chromatographic behaviour. The method was validated using grass samples spiked at the level of 1 mg/kg and gave a detection limit of 0.3 mg/kg for glyphosate and a recovery of 82.4% with RSD of 10.3%.

Review methods used over last morn than 10 years for pretreatment and determination residues of glyphosate and aminomethyl phosphonic acid. Methods of pretreatment are included extraction, purification, derivatization and so on. GC and HPLC are maily used in the determination, and HPLC is better method.