我国已经步入老龄化社会[1],老年人随着年龄增长常多病共存,常会出现多种老年综合征与慢性疾病叠加、多重用药等问题[2].国外研究数据表明,超过半数老年患者合并3种或更多的慢性疾病[3],如何综合管理老年患者已成为目前老年病科医师、全科医师面临的极大挑战.本文对1例卵巢腺癌合并腹腔转移和多器官功能衰竭高龄患者的综合管理进行了报道.

OBJECTIVE:To investigate the heterogeneity of human breast cancer cells, their influence on biological behavior of tumor cells and clinical implications.METHODS:The subpopulations of MCF-7 breast cancer cells were isolated by Percoll gradient centrifugation. DNA content and cell cycle distribution were detected with flow cytometry. Tumor chemosensitivity analysis was performed with MTT assay.RESULTS:Heterogeneity was observed in DNA content and cell cycle distribution among four subpopulations of breast cancer cells, which were related to their proliferation ability and chemosensitivity results.CONCLUSION:Hereditary instability and intrinsic characteristics of most tumor cells, not only lead to tumor progression and heterogeneity but also cause the loss of monoclonality and the generation of subclones. Further study on some profiles of tumor heterogeneity such as DNA content, cell cycle distribution and their influence on tumor proliferation and chemosensitivity may very well improve the clinical treatment.