Objective To investigate the effects of Soy isoflavones on the expression of liver receptor homolog-1 (LRH-1) gene within aging ovary of rats and ovarian granulosa cell cultured in vitro treated with Genistein,which is a major active component of SI.Methods The animal model of perimenopause rats was established by unforced aging,were treated by intragastric administration ( ig ) with low (50 mg/kg),moderate (158 mg/kg) and high (500 mg/kg) dose of SI for 8 weeks.Expression of LRH-1 mRNA were detected by in situ hybridization and RT-PCR,respectively.Female Wistar rats were injected subcutaneously with pregnant mare serum gonadotrophin (PMSG),ovarian granulosa cells were collected and incubated for 24h,and then granulosa cells were administered with genistein (0,0.1,1,5,10,100 μmol/L) and ICI182,780( 1 μmol/L) for 24 h.LRH-1 mRNA expression levels were assessed by RT-PCR.Results Compared with the control model group ( 0.460 + 0.082 ),LRH-1 mRNA in the aging ovary(low dose 0.563 ±0.037,moderate dose 0.926 +0.127,high dose 1.223 ±0.134),were ihcreased significantly( P ＜ 0.05 ).1 ～ 10 μmol/L Genistein could increase the expression of LRH-1mRNA ( low dose 0.844 ± 0.042.Moderate dose 0.879 ± 0.056,high dose 0.882 ± 0.079 ) in the rats granulosa cells treated for 24 h ( P ＜ 0.05 ).UP - regulation of the expression of LRH-1 mRNA by 1 ～ 10 μmol/L Genistein was not in fluenced by ICI182,780 (1 μ mol/L,pretreated for 30 min).Conclusion Soy Isoflavones could up-regulate the expression of LRH-1 mRNA in aging ovaries.1 ～ 10 μmol/L Genistein could up-regulate the expressions LRH-1 mRNA of granulosa cells in rat ovaries,which was probably not mediated by classical ER pathway.

目的:观察大豆异黄酮(SI)对围绝经期大鼠卵巢组织形态学变化及SPARCmRNA表达的影响,探讨大豆异黄酮作用于卵巢的分子机制.方法:自然老化法建立围绝经期大鼠动物模型.选取12月龄初老雌性Wistar大鼠,分别给予低剂量、中剂量、高剂量大豆异黄酮灌胃处理8周.采用光镜HE染色和电镜观察大鼠卵巢形态学变化,半定量RT - PCR检测卵巢SPARCmRNA表达.结果:与青年组相比,围绝经期大鼠的卵巢形态结构不良；激素分泌活性低下；卵巢SPARCmRNA表达显著降低(P＜0.01).各剂量SI均可改善衰老卵巢形态,上调围绝经期大鼠卵巢SPARCmRNA的表达(P＜0.05或P＜0.01).结论:改善衰老卵巢形态、上调衰老卵巢SPARCmRNA表达可能是大豆异黄酮改善围绝经期卵巢微循环血管功能的机制之一.