The synergistic antibacterial activity of lysostaphin and polycations of different chemical structures against Staphylococcus aureus has been shown. Polycations improved the lytic activity of lysostaphin against the peptidoglycan of staphylococci. It is proposed that this resulted in decreased binding of positively charged lysostaphin with S. aureus cell-wall teichoic acids. These data provide an opportunity to search for polycations that would amplify the synergistic effect of lysostaphin or other antibacterial proteins against staphylococci.

Показан эффект синергизма лизостафина и поликатионов различной химической структуры при проявлении антибактериальной активности против Staphylococcus aureus. Поликатионы усиливали литическое действие лизостафина на пептидогликан стафилококков. Высказано предположение, что при этом уменьшалось связывание положительно заряженного лизостафина с тейхоевыми кислотами клеточных стенок S. aureus. Полученные данные открывают перспективы для поиска поликатионов, усиливающих эффект синергизма при действии лизостафина и других антибактериальных белков на стафилококки.

The ability of chitosan polymer enhance lysostaphin lysis of the cell walls of staphylococci was demonstrated. It has been found that the potentiation of lysostaphin activity depends on the molecular weight of chitosan and the pH of the reaction medium. In mildly acidic conditions chitosan had the greatest activating effect with an average molecular weight and in weakly alkaline conditions low molecular weight sample had the greatest activating effect. The results of the lead to the assumption that one of the mechanisms of antibacterial action of chitosan, which is to enhance the degradation of the cell wall of the bacteria in the presence of their own autolysins by chitosan polymer.