Поплавская, Е. А. Морфологические изменения сустентоцитов семенников крыс при воздействии бактериального липополисахарида E. coli / Е. А. Поплавская, Р. Е. Лис // Достижения и инновации в современной морфологии: сб. тр. науч.-практ. конф. с междунар. участием, посвящ. 115-летию со дня рожд. акад. Д. М. Голуба, Минск, 30 сент. 2016 г. : в 2 т. / М-во здравоохранения Респ. Беларусь ; Белорус. науч. о-во морфолог. ; Белорус. гос. мед. ун-т ; под ред. П. Г. Пивченко, Н. А. Трушель. - Минск : БГМУ, 2016. - Т. 2. - C. 98-101.

The aim of the study was to evaluate structural changes in the testes of rats on the third day after exposure to the bacterial lipopolysaccharide gram-negative microorganism Escherichia coli. Bacterial lipopolysaccharide Escherichia coli (serotype 0111: B4, manufactured by Sigma, USA) was administered to male rats at a dose of 50 mkg/kg intraperitoneally one time. Research methods included histological, morphometrical, electron microscopy. In the experimental animals there were detected a decrease in the number of interstitial endocrine cells by 13.85% and a decrease in the area of their nuclei by 23.06%; a decrease in the number of sustentocytes by 25.65% and reduction of the area of their nuclei by 17.15%; a decrease in the number of spermatocytes by 26.54%. Electron microscopic examination revealed swollen basement membrane of the tubules and ultrastructural changes in sustentocytes and spermatocytes. Single administration of E. coli bacterial lipopolysaccharide to male rats at a dose of 50 mg/kg i.p., causes on the 3rd day after exposure development of pathological changes in the testes of the animals, which results in a reduction of interstitial endocrinocytes; reduction in the amount of sustentocytes and spermatocytes while reducing the size of their nuclei; changes in the ultrastructure of spermatogenic epithelium in the form of large numbers of phagolysosomes in the cytoplasm of sustentocytes and reduction of cristae in mitochondria.

The experiment on 21 white male rats and 84 pregnant white female rates was carried out to investigate potential effect of inguinal hernioplasty using alloplastic materials on fertility of white male rats and possible embryotoxicity of the endoprostheses. Two experimental, one control and one intact group were formed of male rates. Operation of double-sided hernioplasty using polypropylene endoprosthesis was modeled in the first group (n=6). In the second group (n=5) double-sided hernioplasty using polytetrafluorethylene endoprosthesis was modeled. Hernioplasty in male rats of control group (n=5) was modeled without using endoprosthesises, the plasty was carried out with caproic filament. Male rates of intact group (n=5) did not undergo any surgery. The male rats of experimental groups were mated with intact female rats to detect the influence of inguinal hernioplasty using alloplastic materials on fertility of male rates and possible embryotoxic action of implants. The male rats of intact group were mated with intact females as well. Results of the research indicate the absence of negative effect of inguinal hernioplasty method using alloplastic materials on fertility of the white male rats and the absence of endoprostheses embryotoxicity.