Objective To assess the diagnosis value of epithelial membrane antigen (EMA) immunocytochemistry examination in meningeal carcinomatosis.Methods The routine cerebrospinal fluid cytologic examination and EMA immunocytochemistry examination of 23 cerebrospinal fluid specimens from 14 patients with definitive meningeal carcinomatosis were analyzed,retrospectively.Results The positive rate of routine cerebrospinal fluid cytologic examination and EMA immunocytochemistry examination were 39.13%(9/23)and 86.96%(20/23),respectively. There was a significant difference between two results ( P0.01). The two positive rates were 28.57% (4/14) and 78.57%(11/14)in the first cerebrospinal fluid specimens,there was also a significant difference between the results ( P0.05). Conclusion The sensitivity of EMA immunocytochemistry examination is superior to routine cerebrospinal fluid cytologic examination in the diagnosis of meningeal carcinomatosis.

Objective To increase the sensitivity of cytologic detection of malignant neoplasms in cerebrospinal fluid(CSF).Methods All 21 CSF specimens from 13 patients with definitive meningeal carcinomatosis were tested by routine cytologic and immunocytochemical examinations,respectively.The positive rates of these two examinations were compared.Results The initial positive of routine CSF cytology was 4 cases,and both the initial positive of immunocytochemistry for anti-carcinoembryonic antigen(CEA) and anti-epithelial membrane antigen (EMA) were 10 cases.There was significant difference between the positive rate of routine CSF cytology and the positive rate of immunocytochemistry for anti-CEA(P0.05),but there was no significant difference between the positive rate of routine CSF cytology and the positive rate of immunocytochemistry for anti-EMA(P0.05) after statistic analysis.The total positive rate of routine CSF cytology was 42.86%(9/21) and the total positive rate of immunocytochemistry for anti-CEA was 80.95%(17/21).There was a significant difference between the two rates(P0.05).The total positive rate of immunocytochemistry for anti-EMA was 85.71%(18/21),and also there was a significant difference between the positive rate of immunocytochemistry for anti-EMA and the positive rate of routine CSF cytology(P0.01).Conclusion The sensitivity of immunocytochemical examination was superior to that of the routine cerebrospinal fluid cytologic examination in diagnosing the meningeal carcinomatosis.

一,A型选择题 1.下列哪项不符合原红细胞的特点( ) A胞体外缘可见伪足突起 B染色质呈粗颗粒状 C核仁较大,界限不清 D胞质呈均匀淡蓝色 E核仁3个以上者一般少见

目的探讨儿童急性淋巴细胞白血病(ALL)免疫表型与无病生存期(EFS)的关系.方法对48例初发未治疗的儿童ALL,用免疫酶标法测其骨髓肿瘤细胞的免疫表型.治疗采用1993年北海会议制定的<小儿白血病诊疗建议>.结果 40例为My-BL,其首次诱导完全缓解(CR)率97.5%,化疗2年内EFS 82.5%,3年内EFS72.5%.4例My+BL,首次诱导CR率50%,化疗2年内EFS 25%.3例My-TL首次诱导均CR,但EFS与My+BL基本类似.结论目前儿童ALL的免疫表型测定在判断其疗效与预后方面有着重要作用.

用单克隆抗体(单抗)对白血病细胞作免疫表型分析,目前已成为辅助诊断白血病及鉴别诊断白血病类型的手段之一.我们在1994年1月～2000年1月对83例未经治疗的儿童急性白血病(AL)进行了免疫表型分析.

由中国癌症研究基金会主办,白血病*淋巴瘤杂志社和解放军总医院血液科联合承办的第三届全国难治性白血病学术研讨会定于2001年10月上旬在北京召开.此次会议被列为国家级继续教育项目,颁发一级学会论文证书及国家级继续教育学分.会议将邀请著名专家作专题报告. 征文内容:有关难治性白血病的病因、发病机制、实验研究、临床诊断标准与治疗、造血干细胞移植、护理及预后因素等. 征文要求: 1.征文为全文并附800字左右的文摘,内容包括:研究目的、方法、结果和结论; 2.征文请详细附有工作单位、作者姓名,邮编、联系地址与电话号码; 3.征文需打印或字迹清楚地书写(最好寄软盘)请自留底稿; 4.征文请于2001年6月30日前至山西省太原市职工新街3号<白血病*淋巴瘤>杂志编辑部,邮编:030013,请在信封上注明"会议征文”字样. 5.联系电话:0351(6450389),传真:0351-4424182. 6.E-mail:BXBZJBJB@263.net 

慢性粒细胞性白血病(CGL)是一种起源骨髓造血多能干细胞的克隆性异常增殖疾病[1,2].对此无论从形态学、细胞化学和遗传学等方面均有其特性显示.但当进入急变期后,由于此病起源于干细胞水平,所以对急变细胞进行准确的定型尤其对过氧化物酶(POX)呈阴性病例是非常重要的.病例本室运用细胞免疫酶法,对29例POX呈阴性的CGL急变细胞进行了细胞表型检测,现在报道如下.

目的探讨B淋巴细胞相关抗原在急性B细胞型淋巴细胞白血病(B-ALL)细胞表面的表达及其与细胞形态和治疗的关系. 方法采用ABC-AP免疫组化法结合选用多种B淋巴细胞相关抗原的克隆性抗体对301例初发(B-ALL)患者的白血病细胞进行细胞表面抗原检测. 结果 B-ALL患者的白血病细胞CD19、HLA-DR呈高阳性率表达;而CD20的阳性表达率较低.阳性表达率与FAB分类分型有一定关系.其中L1型CD 10细胞有高表达率近90%;L2型CD10细胞表达率为63.5%;L3型细胞则无CD10阳性表达. 结论结果均与B-ALL中B细胞的异常分化及细胞所处的阶段有相当大的关系.