Circular RNA (circRNA)-associated competing endogenous RNA (ceRNA) mechanism have emerged as critical mechanism in cancer initiation and progression. However, the roles of the circRNA-microRNA (miRNA)-messenger RNA ceRNA network in osteosarcoma are still not fully characterized. In this study, therefore, circ_0078767-related ceRNA mechanism in osteosarcoma was studied. Bioinformatics tools primarily identified differentially expressed circRNAs and their downstream miRNAs in osteosarcoma, implying the potential interaction between circ_0078767, miR-330-3p, and cyclin-dependent kinase 14 (CDK14) in this malignancy, which were further verified by means of RNA immunoprecipitation, RNA pull-down, and dual-luciferase reporter gene assays. Aberrant abundance of circ_0078767 was found in both osteosarcoma tissues and cells, relating to dismal prognosis in patients with osteosarcoma. Functionally, circ0078767 strengthened the proliferation, invasiveness, and migration of osteosarcoma cells, which could be neutralized by miR-330-3p. Additionally, miR-330-3p targeted and decreased CDK14 expression whereby motivating the malignant phenotypes of osteosarcoma cells. Through in vivo experiments, we further confirmed that circ_0078767 targeted miR-330-3p to upregulate CDK14, whereby strengthening the in vivo tumorigenic and metastatic ability of osteosarcoma cells. Circ_0078767 promotes the occurrence and development of osteosarcoma by upregulating CDK14 in a miR-330-3p-dependent manner.

目的 探讨玻璃酸钠联合盐酸氨基葡萄糖治疗老年膝关节骨性关节炎(KOA)的疗效.方法 选取2017年3月—2018年3月该院收治的老年KOA患者100例,随机分为治疗组和对照组,各有50例患者.两组患者均接受常规抗炎镇痛治疗.给予对照组患者膝关节腔内注射玻璃酸钠注射液,治疗组患者在此基础上加用盐酸氨基葡萄糖片.两组均治疗5周,其后评价患者的疼痛程度和临床疗效.结果 治疗组患者治疗后3、6个月的VAS评分显著低于对照组,差异有统计学意义(P<0.05).治疗组患者的治疗总有效率(88.0％)显著高于对照组(70.0％),差异有统计学意义(P<0.05).结论 氨基葡萄糖胶囊联合玻璃酸钠治疗老年KOA的疗效显著优于单纯玻璃酸钠治疗,明显降低VAS评分,安全性较高.

Wogonoside possesses anti-oxidative, anti-inflammatory, anti-allergy and anti-tumor properties. The aim of the present study was to evaluate whether wogonoside alleviates spinal cord injury (SCI)-induced inflammation via nuclear factor (NF)-κB and nucleotide-binding oligomerization domain-like receptor family pyrin domain containing 3 (NLRP3) inflammasome activation. Sprague-Dawley rats were positioned in the jaws of a calibrated aneurysm clip with a closing pressure of 55 g. The jaws were placed on the dorsal and ventral surfaces of the spinal cord and left in place for 1 min. SCI rats were treated with 12, 25 and 50 mg/kg wogonoside. Following this, the locomotor function was assessed using the Basso Beattie Bresnahan scale. The water content of the spinal cord was measured, tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 levels were assessed and western blot analysis was performed to evaluate the expressions of NF-κB and NLRP3. Wogonoside was demonstrated to significantly ameliorate the SCI-induced reduction in Basso Beattie Bresnahan score (P<0.01) and significantly reduce the water content of the spinal cord in rats with SCI-induced inflammation (P<0.01). Results also indicated that treatment with wogonoside significantly reduced the levels of IL-1β, TNF-α and IL-6 in rats with SCI-induced inflammation (P<0.01), potentially via the phosphorylation of NF-κB inhibitor α. Furthermore, treatment with wogonoside inhibited the expressions of toll-like receptor 4, NLRP3 and caspase-1 protein in SCI model rats (P<0.01). In conclusion, the results of the present study suggest that wogonoside alleviates SCI-induced inflammation by suppressing NF-κB and NLRP3 inflammasome activation.

Metastasis is the primary cause of mortality in osteosarcoma. Targeting metastasis is a major strategy in osteosarcoma treatment. As a traditional Chinese medicine, Trillium tschonoskii Maxim has been widely used in the therapy of various diseases, including cancer. However, currently there is no evidence regarding the anti-metastasic effect of Paris saponin VII (PS VII), which is extracted from Trillium tschonoskii Maxim, on osteosarcoma cells and its underling mechanisms. The present study aimed to examine the effect of PS VII on the migration and invasion of osteosarcoma cells. Viability and proliferation of osteosarcoma cells were examined by MTT assay. Migration and invasion of osteosarcoma cells was then detected using scratch wound healing assays and Transwell assays, respectively. Additionally, the expression of matrix metalloproteinase (MMP)-2 and -9 was determined at the mRNA and protein level following treatment with PS VII. Mitogen-activated protein kinase (MAPK) expression was also detected by western blot analysis. Finally, an inhibitor of p38 MAPK was used to verify the effect of PS VII on the expression of MMP-2 and -9, as well as the migration and invasion osteosarcoma cells. This demonstrated that the proliferation, migration and invasion of the osteosarcoma cells were suppressed following treatment with PS VII. PS VII downregulated the expression of MMP-2 and -9 in a dose- and time-dependent manner. PS VII also exerted its ability to downregulate the phosphorylation of p38 MAPKs. Furthermore, by using a p38 inhibitor, SB203580, the role of PS VII in MMP-2 and -9 expression and osteosarcoma cell invasion was revealed. Taken together, these results demonstrated that PS VII suppresses the migration and invasion of osteosarcoma cells via the p38 MAPK signaling pathway.

BACKGROUND:Bone marrow mesenchymal stem cel s and al ogenic bones are commonly used as seed cel s and scaffolds, respectively, for constructing tissue-engineered cartilage through in vitro co-culture. The loose body of the knee joint can survive in the articular cavity for a long time, and maintain certain characteristics of cartilage tissues. Therefore, the articular cavity can provide a good environment for the growth and development of chondrocytes. OBJECTIVE:To investigate the effect of bone marrow mesenchymal stem cel s co-cultured with al ogenic bone in the articular cavity. METHODS:Bone marrow mesenchymal stem cel s were isolated from five newborn New Zealand white rabbits. One adult New Zealand rabbit was enrol ed to prepare al ogenic bone for co-culture with bone marrow mesenchymal stem cel s. Afterwards, bone marrow mesenchymal stem cel s and al ogenic bone composites were cultured in the articular cavity (intracavitary culture group) or in vitro as in vitro culture group, respectively;the normal cartilage tissues grew in the articular cavity as control group. Cel s were observed by hematoxylin-eosin staining and type II col agen immunohistochemistry staining at 4, 8 and12 weeks of culture. RESULTS AND CONCLUSION:At 12 weeks culture, hematoxylin-eosin staining showed:in the control group, chondrocytes arranged tightly and directional y with red stained cytoplasm and cartilage matrix as wel as blue nuclei;in the intracavitary culture group, the scaffold was mostly absorbed and chondrocytes grew into the scaffold in a certain direction with smaller shape, while cytoplasm and cartilage ma trix were red stained, blue nuclei appeared;in the in vitro culture group, abundant chondrocytes proliferated in a disordered arrangement. Immunohistochemistry staining showed:the absorbance ( A) values in the intracavitary culture group showed a continuous increase, but no obvious change was in the other two groups. Moreover, at 4, 8 and 12 weeks of culture, A values in the control group and intracavitary culture group were significantly higher than that in the in vitro culture group (P<0.05);at 4 and 8 weeks, A value in the intracavitary culture group was significantly lower than that in the control group (P<0.05), but at 12 weeks, there was no significant difference in A value between the two groups (P>0.05). These results suggest that the tissue-engineered cartilage can be constructed by bone marrow mesenchymal stem cells co-cultured with allogenic bone under in vitro and in vivo environment, especially in the articular cavity.

作者自2009---2011年共收治了25例（男16例，女9例）膝关节内侧副韧带损伤的病例，均行切开锚钉固定修补术，随访平均14个月，切口均甲级愈合，优良率为96％。此方法手术简单，值得推广使用。

目的 评价侧卧位及指法定位股骨近端髓内钉微创治疗股骨转子间骨折的临床疗效.方法 股骨转子间骨折患者73例,按手术时间早晚分为传统组30例和微创组43例.均予以闭合复位,微创组采用小切口,手指引导在股骨大转子顶端内侧开孔扩髓,克氏针辅助不稳定骨折复位及临时固定;传统组按常规方法置入髓内钉.分别记录手术时间、切口长度、操作方法、失血量以及术中X线曝光时间、骨折复位情况、骨折愈合时间、髋关节功能恢复等情况并进行比较.结果 微创组平均手术时间、切口长度、失血量、X线曝光时间分别为(49.41±19.63)min、(2.48±1.05)cm、(73.72±40.40)ml、(15.51±6.52)s,明显少于传统组的(92.17±27.76)min、(12.63±4.12)cm、(354.67±84.01)ml、(55.00±30.83)s,差异有统计学意义(P＜0.01).2组患者骨折复位优良,微创组解剖复位占88.37%,功能复位占11.63%,优良率100%;传统组解剖复位53.33%,功能复位占40.00%,复位欠佳占6.67%,优良率93.33%,差异有统计学意义(P＜0.05).临床愈合时间微创组(12.33±2.47)周,传统组(13.47±2.50)周,差异无统计学意义(P＞0.05).2组患者功能恢复满意,髋关节功能优良率微创组97.67%,传统组93.33%,差异有统计学意义(P＜0.05).结论 侧卧位及指法定位股骨近端髓内钉微创治疗股骨转子间骨折是理想的内固定方法.

髋臼骨折属关节内骨折,要求解剖复位,有效固定,早期锻炼,疗效才能满意.我们自1991年始对移位较大的36例髋臼骨折行内固定术,疗效较好,现报告如下:

通过收治体内异物病人348例,对体内异物的取出进行探讨. 1 取异物以前一定要摄X线片,一般摄正侧位片,必要时摄特殊位片,做好立体定位,判断异物距体表的距离及距其最近的骨性标志的距离和方位.若不事先定好位置,就是做大切口也往往要失败.