Background: Oligoasthenozoospermia (OA) is a common cause of male infertility. Modified Liuwei Dihuang Decoction (MLWDH) is an improved version of Liuwei Dihuang Decoction (LWDH), a traditional Chinese medicine prescription, which has demonstrated significant therapeutic effects against OA. This study aims to evaluate the protective effects of MLWDH against OA and elucidate its underlying molecular mechanisms. Methods: The constituents of MLWDH were identified via UPLC-HRMS and compound databases (TCMSP, HERB). Network pharmacology analysis was conducted to predict potential therapeutic targets and associated signaling pathways. In vivo, a CP-induced mouse model of OA was established to evaluate the therapeutic efficacy of MWDH by assessing testicular and epididymal indices, sperm quality, histopathological changes and serum hormone levels. Oxidative stress markers, including MDA, SOD, GSH and NO, were measured using commercial assay kits. The underlying molecular mechanisms, particularly those related to oxidative stress and inflammation (PI3K, Akt, Nrf2, Keap1, HO-1, NQO1, NF-κB, TNF-α, IL-6), were further elucidated by RT-qPCR, Western blot, and immunofluorescence. Results: A total of 345 major bioactive compounds were identified in MLWDH. Network pharmacology and molecular docking analyses indicated that MLWDH exerts its effects primarily through the PI3K/AKT signaling pathway. MLWDH administration in vivo significantly improved sperm count, motility, and morphology, while also increasing serum levels of testosterone, FSH, and LH. Moreover, MLWDH significantly mitigated oxidative damage, as evidenced by decreased MDA concentrations and elevated levels of GSH, NO and SOD. Mechanistic investigations further substantiated that MLWDH enhanced PI3K/AKT/Nrf2 signaling while inhibiting NF-κB signaling in OA mice. Conclusions: Our findings suggest that MLWDH ameliorates OA in a preclinical mouse model by improving sperm quality and testicular function, potentially via activation of the PI3K/AKT/Nrf2 signaling pathway and the inhibition of NF-κB signaling, thereby alleviating oxidative stress and inflammatory responses.

To establish a novel murine model conducive to simian immunodeficiency virus (SIV) replication in vivo, male BALB/c nude mice, aged three weeks, were administered a single intraperitoneal inoculation of SIV-infected MT-2 cells (derived from human T-cell leukemia). Subsequently, alterations in plasma viral load and the engraftment of MT-2 cells in vivo were examined, while immunological changes were assessed via ELISA and flow cytometry analysis. All of the mice in the experimental group exhibited detectable plasma viral loads in their peripheral circulation, largely due to the proliferation of SIV-infected MT-2 cells within the T-cell-deficient environment of nude mice, resulting in colonization of the abdominal cavity and lymph nodes and the subsequent release of free virions to perpetuate the infection. Mice that received highly active anti-retroviral therapy (HAART) treatment demonstrated a statistically significant reduction in plasma viral loads, with HAART administration partially reversing the course of SIV-induced immune dysfunction. Therefore, the model presented in this study offers substantial potential as a robust tool for evaluating antiviral efficacy and immune modulation in an in vivo setting.

PURPOSE:Dietary habit significantly contributes to the initiation and progression of rheumatoid arthritis (RA). Dietary choices intersect with a range of clinical and societal factors. The utilization of cross-sectional methodologies in numerous studies poses a challenge in establishing definitive causality for the noted associations. Moreover, within identical food categories, specific items may elicit diverse effects on the pathogenesis of RA, and the correlation between several prevalent food items or beverages and RA remains inadequately explored. METHODS:We performed a two-sample Mendelian Randomization (MR) study to evaluate the causal impact of 27 distinct dietary habits and RA susceptibility. These dietary-related phenotypes encompass both the relative intake of the four macronutrients (fat, protein, carbohydrates, and sugar) and 23 specific single food intake. Several filtering steps were employed to select eligible genetic instruments strongly associated with each of the traits. The random-effects inverse-variance weighted, weighted median, and MR-Egger methods were employed for MR estimations. Sensitivity analyses and power calculations were executed to ensure the robustness of our study. RESULTS:After rigorous single-nucleotide polymorphism (SNP) filtering procedures, 611 SNPs were included in our ongoing investigations. The consumption of dried fruit, bread, and alcohol emerged as protective factors, while beef, processed meat, and coffee intake were identified as risk factors. The robustness of our study was confirmed through the outcomes of sensitivity analysis and power calculation. CONCLUSION:The ongoing investigations furnish evidence that accentuates the influence of diet on RA disease activity, underscoring the importance of delineating the optimal nutritional lifestyle for RA patients.

ETHNOPHARMACOLOGICAL RELEVANCE:Alcohol misuse persists as a prevalent societal concern and precipitates diverse deleterious consequences, entailing significant associated health hazards including acute alcohol intoxication (AAI). Binge drinking, a commonplace pattern of alcohol consumption, may incite neurodegeneration and neuronal dysfunction. Clinicians tasked with managing AAI confront a dearth of pharmaceutical intervention alternatives. In contrast, natural products have garnered interest due to their compatibility with the human body and fewer side effects. Lingjiao Gouteng decoction (LGD), a classical traditional Chinese medicine decoction, represents a frequently employed prescription in cases of encephalopathy, although its efficacy in addressing acute alcoholism and alcohol-induced brain injury remains inadequately investigated.AIM OF THE STUDY:To investigate the conceivable therapeutic benefits of LGD in AAI and alcohol-induced brain injury, while delving into the underlying fundamental mechanisms involved.MATERIALS AND METHODS:We established an AAI mouse model through alcohol gavage, and LGD was administered to the mice twice at the 2 h preceding and 30 min subsequent to alcohol exposure. The study encompassed the utilization of the loss of righting reflex assay, histopathological analysis, enzyme-linked immunosorbent assays, and cerebral tissue biochemical assays to investigate the impact of LGD on AAI and alcohol-induced brain injury. These assessments included a comprehensive evaluation of various biomarkers associated with the inflammatory response and oxidative stress. Finally, RT-qPCR, Western blot, and immunofluorescence staining were carried out to explore the underlying mechanisms through which LGD exerts its therapeutic influence, potentially through the regulation of the RhoA/ROCK2/NF-κB signaling pathway.RESULTS:Our investigation underscores the therapeutic efficacy of LGD in ameliorating AAI, as evidenced by discernible alterations in the loss of righting reflex assay, pathological analysis, and assessment of inflammatory and oxidative stress biomarkers. Furthermore, the results of RT-qPCR, Western blot, and immunofluorescence staining manifest a noteworthy regulatory effect of LGD on the RhoA/ROCK2/NF-κB signaling pathway.CONCLUSIONS:The present study confirmed the therapeutic potential of LGD in AAI and alcohol-induced brain injury, and the protective effects of LGD against alcohol-induced brain injury may be intricately linked to the RhoA/ROCK2/NF-κB signaling pathway.

Background and Purpose: The previous humanized mouse model for HIV/AIDS study loses the superiority of easy operation and justifiable cost. In this study, an economical and easy-to-operate small animal model supporting SIV replication in vivo was established. Experimental approach: Three-week-old male BALB/c nude mice were transplanted with SIV infected MT-2 cells by single intraperitoneal injection to establish the SIV infection model. The change in plasma viral load and the colonization of MT-2 cells in vivo were investigated. Changes of the immune system were evaluated by ELISA assay and flow cytometry assay. Results: The success rates of this model were 100% and all mice in the model group had detectable plasma viral loads (4.98±0.35 ~ 5.39±0.31 log10 SIV RNA copies / mL) in peripheral blood. It is our speculation that the virus replication in mice was mainly due to the proliferation of SIV-infected MT-2 cells that distributed and colonized in abdominal cavities as well as lymph nodes, releasing free virions to maintain infection. It is worth mentioning that there was a statistically significant downtrend in the plasma viral loads of the HAART group. Administration of HAART somewhat reversed this trend of SIV-associated B cell exhaustion and immune collapse. Conclusions and Implications: Therefore, it is reasonable to believe that the model proposed in this study could be a valuable tool to evaluate antiviral effects and immune regulation efficacy in vivo.

Objective: To explore the mechanism of Weipiling in inhibiting the oxidative stress of gastric ‘inflammation cancer’ transformation in vivo. Methods: A total of 50 Balb/c mice were divided into normal group（10 mice） and model group [40 mice, model group, high-and low-dose group of Weipiling, positive drug group（vitamin B12）, 10 mice in each group].The model of precancerous lesions of gastric cancer were established. After the treatment with corresponding drugs, the overall situation（tail, body shape）, body mass growth, and changes in the structure of gastric mucosa were observed. Nuclear factor E2 related factor 2（Nrf2）/quinone oxidoreductase 1（NQO1）/heme oxygenase-1（HO-1） signal pathway related protein, gene expression and serum SOD value were detected. Results: Compared with the blank group, the tail of the model group was shorter,the color was darker, the body size was significantly reduced, the body mass increased slowly, the gastric mucosa tissue cell dysplasia was obvious, and the gastric mucosa Nrf2/NQO1/HO-1 signal pathway was activated; Compared with the model group,the overall situation of mice in the high-and low-dose groups of Weipiling improved, the structural remodeling and dysplasia of gastric mucosa decreased, and the Nrf2/NQO1/HO-1 signal pathway was inhibited to a certain extent（increased expression）,suggesting that oxidative stress was blocked. Conclusion: Free drinking of MNNG can induce oxidative stress in the gastric mucosa epithelium of mice. Weipiling may block oxidative stress to a certain extent and then inhibit the transformation of gastric ‘inflammation to cancer’, thus playing an anti-gastric precancerous role.

Objective: To explore the protective effect of glycyrrhizin on gastric mucosal injury. Methods:Glycyrrhizin was extracted from the traditional Chinese medicine licorice and identified as authentic, with purity＞90%. Thirty two healthy male SD rats with SPF were randomly divided into blank group, model group, glycyrrhizin group（20 mg/kg） and positive control group（omeprazole, 10 mg/kg）,with 8 rats in each group. The drug was administered by gavage every morning, once a day for 7 days. Except for the blank group, the other groups carried out one-time weight-bearing exhaustive swimming after intragastric gavage for 1 h at the 7th day. All rats were killed after anesthesia, and the gastric ulcer index（UI） was calculated.After HE staining, the tissue morphology of gastric mucosa was observed under light microscope. Enzyme linked immunosorbent assay（ELISA） was used to detect tumor necrosis factor-α（TNF-α）, interleukin-1β（IL-1β）, interleukin-6（IL-6） and other protective factors such as epidermal growth factor（EGF） and prostaglandin E2（PGE2） in gastric mucosa. Results: Compared with the blank group, the UI value and the levels of TNF-α, IL-1β and IL-6 increased significantly（P＜0.01）, and the contents of EGF and PGE2decreased significantly（P＜0.01） in the model group’s gastric mucosa. Meanwhile, the pathological changes of gastric mucosa in the model group were observed. Compared with the model group, the UI value and the levels of TNF-α, IL-1β and IL-6 were significantly decreased（P＜0.01）, and the contents of EGF and PGE2 were significantly increased（P＜0.01） in the glycyrrhizin group’s gastric mucosa, and the pathological damage of gastric mucosa was reduced. Conclusion: Glycyrrhizin can correct the abnormal secretion of factors such as TNF-α, IL-1β, IL-6, PGE2 and EGF in gastric mucosa caused by exercise stress, reduce the occurrence of gastric ulcer, and has an obvious protective effect on gastric mucosal injury. We should promote the application of glycyrrhizin in the development of health food to help protect gastric mucosa.

ETHNOPHARMACOLOGICAL RELEVANCE:Coronavirus Disease 2019 (COVID-19) is a grave and pervasive global infectious malady brought about by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), posing a significant menace to human well-being. Qingfei Paidu decoction (QFPD) represents a pioneering formulation derived from four classical Chinese medicine prescriptions. Substantiated evidence attests to its efficacy in alleviating clinical manifestations, mitigating the incidence of severe and critical conditions, and reducing mortality rates among COVID-19 patients. AIM OF THE STUDY:This study aims to investigate the protection effects of QFPD in mice afflicted with a coronavirus infection, with a particular focus on determining whether its mechanism involves the NLRP3 signaling pathway. MATERIALS AND METHODS:The coronavirus mice model was established through intranasal infection of Kunming mice with Hepatic Mouse Virus A59 (MHV-A59). In the dose-effect experiment, normal saline, ribavirin (80 mg/kg), or QFPD (5, 10, 20 g/kg) were administered to the mice 2 h following MHV-A59 infection. In the time-effect experiment, normal saline or QFPD (20 g/kg) was administered to mice 2 h post MHV-A59 infection. Following the assessment of mouse body weights, food consumption, and water intake, intragastric administration was conducted once daily at consistent intervals over a span of 5 days. The impact of QFPD on pathological alterations in the livers and lungs of MHV-A59-infected mice was evaluated through H&E staining. The viral loads of MHV-A59 in both the liver and lung were determined using qPCR. The expression levels of genes and proteins related to the NLRP3 pathway in the liver and lung were assessed through qPCR, Western Blot analysis, and immunofluorescence. RESULTS:The administration of QFPD was shown to ameliorate the reduced weight gain, decline in food consumption, and diminished water intake, all of which were repercussions of MHV-A59 infection in mice. QFPD treatment exhibited notable efficacy in safeguarding tissue integrity. The extent of hepatic and pulmonary injury, when coupled with QFPD treatment, demonstrated not only a reduction with higher treatment dosages but also a decline with prolonged treatment duration. In the dose-effect experiment, there was a notable, dose-dependent reduction in the viral loads, as well as the expression levels of IL-1β, NLRP3, ASC, Caspase 1, Caspase-1 p20, GSDMD, GSDMD-N, and NF-κB within the liver of the QFPD-treated groups. Additionally, in the time-effects experiments, the viral loads and the expression levels of genes and proteins linked to the NLRP3 pathway were consistently lower in the QFPD-treated groups compared with the model control groups, particularly during the periods when their expressions reached their zenith in the model group. Notably, IL-18 showed only a modest elevation relative to the blank control group following QFPD treatment. CONCLUSIONS:To sum up, our current study demonstrated that QFPD treatment has the capacity to alleviate infection-related symptoms, mitigate tissue damage in infected organs, and suppress viral replication in coronavirus-infected mice. The protective attributes of QFPD in coronavirus-infected mice are plausibly associated with its modulation of the NLRP3 signaling pathway. We further infer that QFPD holds substantial promise in the context of coronavirus infection therapy.

Objective: Observational studies have posited a strong correlation between chronic gastritis (CG) and major depressive disorder (MDD), but the nature of this association remains uncertain, owing to the challenges of establishing the temporal sequence. The present study sought to elucidate the elusive relationship between CG and MDD by employing a bidirectional two-sample Mendelian randomization (MR) approach.Methods: We extracted instrumental variants for MDD and CG from published genome-wide association study data, focusing on individuals of primarily European descent. A comprehensive suite of MR estimations and sensitivity analyses was performed to ensure the robustness of the findings. Each outcome database was analyzed separately in both directions.Results: For MDD and CG, 221 and 5 genetic variants, respectively, were selectively extracted as instrumental variants. The results suggest that MDD is causally associated with an elevated risk of CG (IVW: 23andMe, OR = 1.33; 95% CI = 1.15-1.54; p = 1.06 x 10-4); conversely, no strong evidence was found to corroborate that CG exerts a causal effect on the incidence of MDD (IVW: OR = 1.01; 95% CI = 0.95-1.07; p = 0.68).Conclusions: These findings provide novel insights into the causal relationship between CG and MDD, which may have implications for clinical decision-making in patients with MDD and CG.

目的 探讨健脾化瘀解毒方在体内改善胃癌前病变小鼠脾虚证的作用机制.方法 构建脾虚证胃癌前病变小鼠模型,使用健脾化瘀解毒方干预后,观察小鼠体质量增长及摄食情况、脾脏组织结构变化、脾/胸腺指数、并检测AKT/p38信号通路相关蛋白表达情况.结果 与空白组比较,模型组小鼠体质量增长缓慢,摄食明显减少,脾脏指数减小,胃黏膜AKT/p38信号通路被激活;与模型组比较,健脾化瘀解毒方干预组小鼠脾虚症状得到改善,脾脏组织结构重塑,且AKT/p38信号通路受到一定程度抑制.结论 MNNG可诱导胃癌前病变小鼠出现脾虚症状,健脾化瘀解毒方可能在一定程度抑制AKT/p38信号通路进而改善胃癌前病变小鼠脾虚症状,与其以健脾法为基础的治法相符合,进而发挥抑制甚至逆转胃癌前病变的作用.

目的 对多中心岭南胃痞病患者住院病历数据的证候及用药情况进行回顾性研究,总结其中医证治规律.方法 选取2010年至2019年广州中医药大学第一附属医院、广东省中医院、广东省第二中医院、广州市中医院、南方医科大学珠江医院、深圳市中医院、江门五邑中医院收治的胃痞病患者的住院病历诊疗数据,回顾性搜集整理患者的年龄、性别、病程、主要症状、舌脉象、中医证型、用药情况等,并对数据进行规范化处理.计数资料采用频数及百分比进行描述性分析.采用Apriori算法对药物-药物进行三阶关联规则分析,并对统计频数较高的中药进行系统聚类分析.结果 共有949例患者符合纳入标准,患者发病年龄主要在50～59岁,女性占比大于男性.岭南胃痞病患者的入院时间以惊蛰节气多见,临床主要表现为上腹部不适,中医证型以脾胃气虚证、肝郁脾虚证、脾胃湿热证为主,中药使用多为补虚类、清热类和活血祛瘀类;根据关联规则分析形成木香+茯苓+白术、砂仁+党参+白术、木香+党参+白术等主要药物组合,并聚类形成以健脾、行气、活血等为核心的新处方7个.结论 岭南胃痞病具有以脾虚为本,兼夹湿热、气滞、血瘀等特点.在运用健脾药物的同时,宜适当佐以行气、清热、化湿、活血类药物,并结合发病的节气特点,做到"未病先防,既病防变".

Modified Lvdou Gancao decoction (MLG), a traditional Chinese medicine formula, has been put into clinical use to treat the diseases of the digestive system for a long run, showing great faculty in gastric protection and anti-inflammatory, whereas its protective mechanisms have not been determined. The current study puts the focus on the protective effect and its possible mechanisms of MLG on ethanol-induced gastric lesions in mice. In addition to various gastric lesion parameters and histopathology analysis, the activities of a list of relevant indicators in gastric mucosa were explored including ALDH, ADH, MDA, T-SOD, GSH-Px, and MPO, and the mechanisms were clarified using RT-qPCR, ELISA Western Blot and immunofluorescence staining. The results showed that MLG treatment induced significant increment of ADH, ALDH, T-SOD, GSH-Px, NO, PGE2 and SS activities in gastric tissues, while MPO, MDA, TNF-α and IL-1β levels were on the decline, both in a dose-dependent manner. In contrast to the model group, the mRNA expression of Nrf-2 and HO-1 in the MLG treated groups showed an upward trend while the NF-κB, TNFα, IL-1β and COX2 in the MLG treated groups had a downward trend simultaneously. Furthermore, the protein levels of p65, p-p65, IκBα, p-IκBα, iNOS, COX2 and p38 were inhibited, while Nrf2, HO-1, SOD1, SOD2 and eNOS were ramped up in MLG treatment groups. Immunofluorescence intensities of Nrf2 and HO-1 in the MLG treated groups were considerably enhanced, with p65 and IκBα diminished simultaneously, exhibiting similar trends to that of qPCR and western blot. To sum up, MLG could significantly ameliorate ethanol-induced gastric mucosal lesions in mice, which might be put down to the activation of alcohol metabolizing enzymes, attenuation of the oxidative damage and inflammatory response to maintain the gastric mucosa. The gastroprotective effect of MLG might be achieved through the diminution of damage factors and the enhancement of defensive factors involving NF-κB/Nrf2/HO-1 signaling pathway. We further confirmed that MLG has strong potential in preventing and treating ethanol-induced gastric lesions.

The molecular detection of SARS-CoV-2 is extremely important for the discovery and prevention of pandemic dissemination. Because SARS-CoV-2 is not always present in the samples that can be collected, the sample chosen for testing has inevitably become the key to the SARS-CoV-2 positive cases screening. The nucleotide amplification strategy mainly includes Q-PCR assays and isothermal amplification assays. The Q-PCR assay is the most used SARS-CoV-2 detection assay. Due to heavy expenditures and other drawbacks, isothermal amplification cannot replace the dominant position of the Q-PCR assay. The antibody-based detection combined with Q-PCR can help to find more positive cases than only using nucleotide amplification-based assays. Pooled testing based on Q-PCR significantly increases efficiency and reduces the cost of massive-scale screening. The endless stream of variants emerging across the world poses a great challenge to SARS-CoV-2 molecular detection. The multi-target assays and several other strategies have proved to be efficient in the detection of mutated SARS-CoV-2 variants. Further research work should concentrate on: (1) identifying more ideal sample plucking strategies, (2) ameliorating the Q-PCR primer and probes targeted toward mutated SARS-CoV-2 variants, (3) exploring more economical and precise isothermal amplification assays, and (4) developing more advanced strategies for antibody/antigen or engineered antibodies to ameliorate the antibody/antigen-based strategy.

At present, four operating modes have been explored for physical-medical integration in China: hospital health guidance center, sports club health guidance, community health monitoring center and industry-university-research cooperation on the integration of physical and medical services. However, physical-medical integration is still in its infancy, the cultivation of physical-medical integration talents has been a key to the deep development of "physical-medical integration". The researches show that the current training mode of integrative talents mainly focuses on on-the-job training, which is faced with many problems such as serious shortage of specialized talents, imperfect certification system and single talent training mode. It is suggested that the training system, the curriculum system, the public opinion system and the professional standards should be improved so as to realize the scientific and sustainable development of physical-medical talents.

Objective This research aimed at better understanding the histopathological development of precancerous lesions of gastric cancer (PLGC) and organelle ultrastructure changes. Methods Sprague-Dawley rats were randomly assigned to the model and control groups. Model rats drank N-methyl-N′-nitro-N-nitrosoguanidine solution, while control rats drank pure water ad libitum. At 1, 3, 5, 6, and 8 months after the start of feeding, eight rats were randomly chosen from each group, and gastric mucosa tissues were removed for histopathological analysis. H&E staining was applied to analyze the pathological histological structure of the rat gastric mucosa via a light microscope, and the ultrastructural changes were observed via a transmission electron microscope. Results Gastric mucosal pathologies of model rats such as mucosal atrophy, intestinal metaplasia, inflammatory lesions, and even intraepithelial neoplasia deteriorated over time. The endoplasmic reticulum gap widened, the mitochondrial endothelial cristae were disrupted, the nuclear membrane thickened, and chromatin condensed with heterotypic alterations in the main and parietal cells. Additionally, endothelial cell enlargement and thickening of the microvascular intima were seen. Conclusion Our research showed that the PLGC progression of rats is correlated with the pathological alteration axis of “normal gastric mucosa-gastric mucosa inflammatory changes-intestinal metaplasia with mild dysplasia-moderate to severe dysplasia.” Ultrastructure analysis of model rats is compatible with the structural changes in the gastric mucosa with spleen deficiency and blood stasis. The pathological evolutionary axis and ultrastructural analysis are helpful for evaluating potential novel herbal therapies for PLGC.

目的:利用文献研究及靶点网络方法挖掘经验用穴足三里穴、中脘穴防治应激性溃疡的作用机制,并进行体内实验验证.方法:检索2010年1月至2021年7月间CNKI、万方、维普、PubMed、Web of Science数据库中关于足三里穴、中脘穴治疗疾病的文献,获取穴位治疗疾病的靶点,构建穴位-靶点网络图;利用GeneCards数据库获取应激性溃疡的疾病作用靶点,构建PPI蛋白互作网络并进行KEGG通路富集分析.构建应激性溃疡大鼠模型,观察足三里穴、中脘穴预针刺对应激性溃疡大鼠的影响.结果:最终纳入文献172篇,共涉及317个靶点;PPI蛋白互作获得53个关键蛋白;KEGG通路富集得到54条信号通路,涉及TNF信号通路、HIF-1信号通路、NOD样受体信号通路、Toll样受体信号通路等.大鼠体内实验显示,与模型组比较,针刺组、抑制剂组均可显著改善大鼠胃黏膜溃疡情况,降低TLR4、Myd88、NF-κB、NLRP3、IL-1 β的水平(P＜0.01,P＜0.05).结论:足三里穴、中脘穴防治应激性溃疡的机制可能与NOD样受体信号通路、Toll样受体信号通路TLR4、Myd88、NF-κB、NLRP3、IL-1β等关键靶点有关.

目的 探讨半夏生物总碱对运动氧化应激大鼠胃黏膜的保护作用及机制.方法 将32只SD大鼠随机分为空白组、模型组、半夏生物总碱组(20 mg·kg-1)和阳性对照组(奥美拉唑,10mg·kg-1),每组8只.灌胃给药,每日1次,连续7d.第7天灌胃给药1h后,进行一次性负重力竭游泳运动造模.麻醉后处死所有大鼠,计算胃溃疡指数(UI),采用HE染色法观察胃黏膜组织病理形态;采用酶联免疫吸附法(ELISA)检测血清一氧化氮(NO)、丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)等氧化应激指标水平,以及胃黏膜组织胃泌素(GAS)、胃动素(MTL)含量.结果 与空白组比较,模型组大鼠的UI值与胃黏膜组织GAS、MTL含量,以及血清NO、MDA含量均显著升高(P＜0.01),血清SOD、GSH-Px水平显著降低(P＜0.01).与模型组比较,半夏生物总碱组大鼠的UI值与胃黏膜组织GAS、MTL含量,以及血清NO、MDA含量均显著降低(P＜0.01),血清SOD、GSH-Px水平显著升高(P＜0.01).结论 半夏生物总碱具有抗氧化作用,能纠正运动应激导致的胃黏膜组织GAS、MTL分泌异常状态,逆转运动氧化应激对胃黏膜的损伤,减少胃溃疡的发生.

肺康复应用于慢性阻塞性肺疾病的治疗已得到广泛研究,其疗效也得到肯定,但对其他慢性呼吸系统疾病的疗效尚不明确,由于相关高质量研究较少,缺少适用于不同疾病的肺康复方案及康复疗效评价体系.本文通过阅读近年国内外关于肺康复治疗各种慢性呼吸系统疾病的疗效研究,综述研究成果,报告肺康复治疗慢性呼吸系统疾病的研究现状.

幽门螺旋杆菌感染是一种慢性感染引起的相关疾病是各种免疫细胞和化学物质长期作用的结果,加之幽门螺旋杆菌自身酶的作用和基因多样性,导致其可在体内持续定植存活,逃避宿主免疫系统.中医理论中的伏邪"结"病机指邪气伏于内,并与机体胶着难解的病理状态,是邪气伏留、病情迁延的重要原因,这与幽门螺旋杆菌持续定植引起感染甚或致癌的免疫逃逸机制有一定相关性.文章基于伏邪"结"病机理论,从基本概念、病机特点、治疗思路着手,结合幽门螺旋杆菌免疫逃逸机制的现代研究进展,分析两者之间的关联,以期为中医药防治幽门螺旋杆菌提供新思路.

背景:假体周围感染是膝/髋关节置换最常见最严重的并发症之一,占全膝关节置换失败原因的25％,全髋关节置换失败的16％.因此,及时准确地诊断关节假体周围感染具有重要临床意义.目的:评估α防御素和C-反应蛋白对假体周围感染的诊断效应.方法:选取2014年9月到2016年9月初次关节置换或关节翻修手术后疼痛的患者,并检测所选患者滑膜液中α防御素和C-反应蛋白的表达情况,并与肌肉骨骼感染协会(MSIS)标准对比,分别计算检测的阳性预测值和阴性预测值.试验于2020-08-07经广州中医药大学伦理审查委员会批准,审批号K[2020]064.结果与结论:α防御素测试与滑膜液C-反应蛋白阳性联合测试的敏感性为81.1％(95％CI:64.8％-92.0％),特异性为95.9％(95％CI:91.3％-98.5％);滑膜液α防御素和C-反应蛋白联合测试的敏感性为73.0％(95％CI:55.9％-86.2％),特异性为99.3％(95％CI:96.2％-99.9％).提示滑膜液中α防御素和C-反应蛋白可用于关节周围感染的辅助诊断.