Garcinia paucinervis Chun et How is a tree species with important ecological, medicinal, and ornamental value. Studying the impact of climate change on the potential distribution of this species offers important information for resource conservation, population restoration, and sustainability. In this study, the MaxEnt model was used to simulate potential distributions under climate change conditions. Results showed that the precipitation of the driest quarter (Bio-17) ranging from 33.3 to 133.3 mm, the precipitation of the warmest quarter (Bio-18) from 667.67 to 1000 mm, the annual mean temperature (Bio-01) from 18.0 to 24.0 °C, and the annual precipitation (Bio-12) from 1250.0 to 1760.0 mm were four dominant factors affecting the distribution of G. paucinervis. Its suitable habitat in China is the narrowest, and it is located in most regions of Guangxi and Guangdong, the southern region of Guizhou, and the southeastern part of Yunnan Province. In the 2050s and 2070s, the geographical distribution gradually decreased compared to current scenarios. Specifically, most of Guangxi and Guangdong, the southern region of Guizhou, the eastern part of Yunnan adjacent to Guangxi, the southeast region of Sichuan, and the northern region of Hainan were identified as stable suitable habitats for G. paucinervis. Meanwhile, the expanding areas were located only in the western and southern regions of Yunnan, and the contracting areas were in the junction of Guangdong, Fujian, and Jiangxi; among Guizhou, Chongqing, and Hunan; among Anhui, Henan, and Hubei; the southeastern region of Sichuan; the western region of Hubei; and the adjacent area between Chongqing and Sichuan. By the 2070s, the contracting habitats will additionally include the central Guizhou region, the northern regions of Guangdong and Guangxi, the eastern region of Guangdong adjacent to Fujian, and the southern Jiangxi Provinces. Thus, this study highlights the vulnerability of the species and its response to future climate change and provides insights for assessing habitat suitability for conservation management.

Aims We aimed to identify the optimal combination of selenium (from Na 2 SeO 4 ) and secondary elements required for obtaining the best nutritive characteristics and quality of 'Qingcui' plums. Methods Five treatments (Na 2 SeO 4 with MgSO 4 and CaSO 4 ) and a control treatment without Se, Mg, Ca, or S were applied to 2-year-old 'Qingcui' plum trees in a greenhouse. Plum leaves and fruit were analyzed for color, nutrient, and quality characteristics during the growing and deciduous seasons as well as the following year. Results Leaf color parameters, pigment content, and specific weight significantly improved by CaSO 4 addition during the growing and deciduous seasons. Ca and Mg contents during the growth, defoliation, and young leaf stages of the following year exhibited consistent trends under the different treatments, whereas Se and S contents differed according to the season and treatment. Thus, Mg and S showed high mobility and reutilization rates, whereas Ca and S showed poor mobility and reutilization rates. Leaf and fruit nutrient contents decreased in the order of Ca > Mg > S and S > Ca > Mg, respectively. The combination of 77 mg Na 2 SeO 4 , 10 g MgSO 4 , and 19 g CaSO 4 yielded optimal fruit quality in terms of fruit weight, hardness, and soluble solids and fruit Se content. A large amount of Ca was present in the cell wall, whereas S and Mg were mainly observed as soluble components. Conclusions Our study revealed the best combination of Se and secondary elements leading to optimal leaf nutrition and fruit Se content and quality in 'Qingcui' plums.

Selenium (Se) is a beneficial element for plant growth and development. In this study, three-year-old potted 'Qingcui' plums were treated with Na2SeO4 (Se6+) or selenomethionine (SeMet Se2-) to explore the effect of Se on the plant growth, fruit quality, and 5-hydroxytryptophan (5-HTP) metabolism. Flower and fruit numbers, fruit quality and yield, Se content, and 5-HTP metabolites and enzymes were detected. The results showed that the flower and fruit numbers, and yield were significantly increased by the Se application. There were no significant differences in the fruit diameters, fruit mass, edible part ratio, titratable acids, water content, and solid acid ratio among the treatments. The total soluble solids, soluble protein, and malondialdehyde contents under the Se6+ treatment showed no significant difference compared to the Se2- treatment, but they were significantly higher than these under control by 16.71%, 39.13%, and 36.27%, respectively. The Se application markedly increased plant the Se content, and Se contents in the roots and leaves, or the fruits were significantly larger by the Se6+ treatments than the Se2- treatment. The leaves' pigment contents under the Se2- treatments were significantly larger than those under the control or Se6+ treatment. Tryptophan was not significantly influenced, the 5-HTP and 5-methoxytryptophan contents were reduced in the roots, and increased in the leaves, and the serotonin content was only significantly increased in the roots by the Se treatments. The tryptophan hydroxylase and hydroxyindole-O-methyltransferase levels were slightly influenced, and the tryptophan decarboxylase level in the roots or fruits was significantly increased by the Se treatments. The Se application had beneficial effects on the plant growth, fruit quality, and Se content, especially in the Se6+ treatment, and influenced the 5-HTP metabolism.

The sweet cherry (Prunus avium) is among deciduous fruit trees with high economic value and its planting area is gradually expanding. However, little was known about its accurately suitable area in China. Herein, the potential distributions were modeled based on the MaxEnt model under the current conditions. Its performance was excellent, with AUCs >0.9 for model training and testing. The key environmental factors were the thermal factors (minimum temperature of the coldest month (bio06) from -14.5 to 4.5°C, the mean temperature of the warmest quarter (bio10) from 21.0 to 28.0°C), followed by the water factor (the annual precipitation (bio12) from 500 to 1200 mm), indicating that it is not resistant to cold and heat, nor is it resistant to drought or floods. The suitable area in China mainly is found in seven geographical regions including southwest China (eastern Sichuan, northeast and main urban areas of Chongqing, mid-western Guizhou and mid-northern Yunnan), northwest China (mid-southern Shaanxi, southern Ningxia mid-southern and eastern Gansu), northeast China (Coastal region of Liaoning), central China (most of Henan, mid-northern Hubei and central Hunan), north China (Beijing, Tianjing, mid-southern Shanxi), east China (Shanghai, Jiangsu, Shandong, central Zhejiang, central and northern Anhui and eastern Jiangxi) and south China (western Guangxi). Based on statistical analysis, these fourteen provinces or cities, namely, Shaanxi, Beijing, Tianjing, Shanxi, Hebei, Henan, Shanghai, Jiangsu, Shandong, Sichuan, Guizhou, Yunnan, Liaoning and Hubei were the main regions for current development and utilization while for the twelve provinces with higher moderate suitable areas, namely, Chongqing, Guizhou, Yunnan, Shaanxi, Ningxia, Liaoning, Hubei, Hunan, Zhejiang, Anhui, Jiangxi and Guangxi, we should supplement the appropriate irrigation and winter insulation facilities etc. Additionally, Hubei, Hunan, Anhui, also have been identified to have some potentially suitable areas. These information will help avoid the loss of human labor, material, and financial resources and provide a scientific basis for its current introduction, cultivation, and management.

Potential geospatial distribution of species supplies momentous information for species management, especially for some plants with high ecological and economic value. Paris polyphylla Smith is a medicinal plant with analgesic, hemostatic, antiinflammatory and anti-tumor activities. Herein, its potential geospatial distribution was calculated under climate change conditions based on 299 known coordinates and 8 environmental layers selected responsible for affecting species distribution. These performances are highly accurate in simulating its geospatial distribution regions, with the AUCs of greater than or near to 0.9 for model building and testing. The key factors were the mean temperature of the coldest quarter (bio11) >7.0(degrees)C, the most suitable point is 15(degrees)C, the annual mean temperature (bio01) >16.25(degrees)C, the temperature seasonality (bio04) from 0 to 650 and the annual precipitation (bio12) >1400 mm. The suitable geographic distribution mostly is located in southwest China, south China, central China, east China and northwest China including increased suitable area, namely, Nyingchi in Tibet with a remarkable reduction to the period of 2050s or 2070s, indicating that the suitable area of the species has a continuous decreasing trend. However, between 2050s and 2070s, the suitable area of the specie is almost unchanged for same greenhouse gas emission scenarios. More importantly, four newly discovered distribution areas including western China (middle-south Shaanxi and southeast Gansu), central China (central and western Hunan), southwest China (Main urban area of Chongqing) and east China (eastern and western Zhejiang), were discriminated as lost suitable areas. Consequently, we should strictly monitor these four newly discovered-lost suitable areas in the future.

To understand the relationship between the alligator weed.s potential distribution and environment variables, its potential distribution was analyzed by the Maxent model under the current and future environmental conditions. The results showed that model performances are highly accurate in predicating its potential distribution in model training and testing. The dominate factors were precipitation of the driest month, temperature seasonality, altitude and precipitation of the wettest month, with thresholds of 20-110 mm, 550-925, <200 m and 160-300 mm respectively, indicating that the alligator weed is suitable for the water-land interface of various freshwater ecosystems in tropical to warm temperate regions. Its suitable geographic distributions are mainly distributed on eastern, southern, central, southwestern China, and south of Shaanxi and Hebei provinces. With climate change, its suitable distributions areas will first increase and then decrease from now to the 2070s, however the total suitable area increases relative to that in the current conditions. To 2050s, the increased suitable areas are southwestern Shandong and southern Hebei provinces, southwestern Yunnan province, and Nyingchi in Tibet, while to 2070s only in the southwest of Shandong province. Therefore, the current suitable habitats should be given highly attention to prevent from its spreading to these above three increased suitable and adjacent areas (western Sichuan, southeastern Gansu, southern Shaanxi and Shanxi provinces). Taken together, these informations from this study should be a useful guide for management of the species.

Abstract Sustainable practices are inevitable requirements for modern agriculture. Incorporating weed waste into an organic substrate is one of such sustainable agricultural practice. We aimed to investigate whether semi-decomposed, aboveground weed biomass can be used as a substrate for plums. “Qingcui” plums were planted in boxes with semi-composted weeds, and garden soil without composted weeds was used as a control. Plant branch characteristics, leaf antioxidant capacity, leaf nutrition, and numbers of opening flowers and bearing fruits were analyzed. The results showed that treatment with semi-composted weed treatment significantly increased the stem diameter, effective branch number, and effective branch angle. The contents of the reduced glutathione, oxidized glutathione, and the levels of total antioxidant capacity and catalase were significantly higher in semi-composted weeds than in the control group. The macroelement content in the semi-composted weed group was higher than that of the control group, but the microelement contents did not show any significant differences. However, the trace elements and heavy metals showed heterogeneous changes. The Σ DOP of macroelements, microelements, and trace elements in the weed-compost treatment were larger than that in the control. The chlorophyll content in the weed-compost group was significantly higher than that in the control during the leaf abscission period. The leaf weight per area was significantly higher than that of the control, but the growth time was longer. The numbers of total opening flowers and fruit were higher in the semi-composted weed group than in the control group. We demonstrated that semi-composted weeds as substrates have beneficial effects on plum branch characteristics, leaf antioxidant capacity, and generative growth. However, it increases the degree of nutritional imbalance and extends growth time.

'Qingcui' plum fruit becomes soft at harvesting. In this study, we assessed the effect of the following different treatments on various fruit texture parameters: three concentrations of nitrogen (NH4NO3 0%, 0.5% and 1%) and three forms of selenium (selenomethylcysteine [C5H9NO2SSe/SeMeCys], sodium selenate [Na2SeO4] and sele-nomethionine [SeMet]). The fruit texture parameters evaluated were fracture, hardness, cohesiveness, springi-ness, gumminess, chewiness and initial modulus under 4oC. Results showed that the optimal N concentration was 1% and the optimal Se form was SeMeCys. Fruits treated with SeMeCys had similar fruit texture parameters as those of the group under cysteine treatment. We then sprayed the fruit with approximately 2 mL/fruit of the following treatments: five different combinations ratios of Se (SeMeCys; 10 mg/L) and N (NH4NO3; 1%) (2:18; 4:16; 10:10; 16:4; 18:2), after which the fruit was stored at 4oC for 13 days. The parameters of fruit texture, fruit color, and other fruit qualities were measured. Linear correlations were observed between parameters, and main sensory qualities were analyzed. We observed that a combination of Se and N in a ratio of 18:2 produced the best results in terms of fracture, first hardness and second hardness, preventing the softening of the fruit. The param-eters of soluble protein, titratable acid and soluble solids had significant linear relationships with three fixture parameters. The fruit color parameters of a* (greenness) and b* (yellowness) also had significant linear correlations with fracture and first hardness. These findings may offer a promising strategy to prolong fruit storage and main-tain fruit crispness and hardness.

重庆地区土壤硒富集存在较大差异,在一些区域土壤富硒显著,具备发展富硒水果的资源优势,富硒产品开发也取得了较好成效,然而在发展富硒水果过程中也存在一些问题.本文综述了重庆富硒土地开发进展、富硒水果生产现状以及认证渠道和消费途径,并提出研究方向和开发对策.

Abstract Purpose: Root application of low-dose selenate does not significantly influence the melatonin content in potted sweet cherry leaves. We aimed to investigate the effects of high doses of selenate on sweet cherry plants to clarify the physiological and biochemical aspects of adaptive stress. Methods: Different doses of 5-methoxytryptophan was foliar applied on the long branch leaves of the ‘Russia No. 8’, and the potted and field grown sweet cherry plants were treated with 0, 48, 96, 144, and 196 mg/plant. The substance content and enzyme activity involved in tryptophan metabolism were detected and the growth parameters were detected. The C, N, Se, sugar, and soluble protein contentsin mature, old, and fallen leaves in August, and in yong leaves in March of the following year. Results: 15 mg/L of 5-methoxytryptophan significantly increased leaf melatonin content. Selenate significantly increased the melatonin content only at a dose of 96 mg/plant. Doses lower than 96 mg/plant increased plant growth parameters, whereas higher doses decreased plant growth parameter. Different leaf ages of sorbitol, amylaceum, fructose, saccharose, rhamnose, and maltose content was influenced by different Se doses treatments. With an increase in the Se dose, the leaf nitrogen content increased, and the carbon content was influenced slightly in field-cultivated trees. Conclusion: Leaf Se content was mainly distributed in mature leaves in response to low doses of Se and in old leaves in response to high doses of Se; The neat year, different Se dose also influence the leaf nutrition and sugar contents.

甜樱桃味美、营养价值高并含有褪黑素.硒元素是人体必需元素,也是植物有益元素,在提高作物产量、品质、抗逆等方面有着重要作用.富硒高褪黑素甜樱桃将备受消费者的青睐,也是育种科学研究追求的目标之一.然而褪黑素在植物体内的合成代谢途径尚未全面清晰,硒在植物体内的吸收、转运和贮存也未完全明确.本文从甜樱桃的营养、观赏和应用方面明确了甜樱桃具有的价值;从富硒水果的价值、硒对甜樱桃品质及褪黑素的影响方面进行探讨,生产富硒甜樱桃较为容易,而要达到高褪黑素水平有一定的不确定性;从植物体内褪黑素合成途径、甜樱桃褪黑素合成途径、硒对甜樱桃褪黑素合成途径的影响等方面进行探索,明确植物体内褪黑素合成途径具有多样性,硒调控植物体内褪黑素含量具有复杂性,为硒调控甜樱桃褪黑素含量提供一定的理论方向.

The pine wood nematode (PWN), one of the largest alien forestry pests in China, has caused numerous deaths of conifer forests in Europe and Asia, and is spreading to other suitable areas worldwide. Information on the spatial distribution of the PWN can provide important information for the management of this species. Here, the current and future geographical distributions of PWN were simulated in the Sichuan-Chongqing region of China in detail based on the MaxEnt model. The results indicated excellent prediction performance, with an area under curve score of more than 0.9. The key factors selected were the altitude, maximum temperature of the warmest month, annual precipitation, precipitation of the wettest quarter, and minimum temperature of the coldest month, with thresholds of < 400 m, > 37.5 °C, 1100-1250 mm, 460-530 mm and > 4.0 °C, respectively, indicating that the PWN can live in low-altitude, warm, and humid areas. The suitable region for the PWN is mainly concentrated in the metropolitan area, northeast of Chongqing, and the southeastern and eastern parts of Sichuan Province. Most importantly, in addition to their actual distribution area, the newly identified suitably distribution areas A, B, C, and D for the coming years and E, F, G, and H for the period-2041-2060 (2050s) should be strictly monitored for the presence of PWNs. Altogether, the suitable distribution ranges of the PWN in the Sichuan-Chongqing region show an increasing trend; therefore, owing to its inability to disperse by itself, human activities involving pine trees and vectors of the Japanese pine sawyer should be intensively controlled to prevent the PWN from spreading to these newly discovered suitable areas.

为了探索根施有机硒对葡萄抗氧化水平以及色氨酸代谢的影响,以盆栽阳光玫瑰葡萄为试材,采用1.0mg/LSe2-(分别来自分析纯硒代蛋氨酸和主要成分为硒代蛋氨酸的富硒酵母)处理扦插苗和幼树,分析有机硒对葡萄根系和叶片谷胱甘肽氧化循环、抗氧化水平以及色氨酸代谢物质和部分酶活性的影响.结果表明,硒代蛋氨酸处理扦插幼苗对根系谷胱甘肽氧化循环有一定的促进作用,而对其抗氧化水平影响不显著.移栽后期发现经过硒代蛋氨酸处理的幼树叶片的谷胱甘肽氧化循环效率得到进一步提高,抗氧化水平也略高于对照,显著降低了色氨酸含量,而显著提高了 5-羟色胺含量.后期富硒酵母处理对葡萄叶片和根系的褪黑素合成相关酶活性产生一定的影响,其中叶片5-羟色胺-N-乙酰转移酶(SNAT)和芳香烷基胺-N-乙酰基转移酶(AANAT)活性均高于根系,而N-乙酰-5-羟色胺-O-甲氧基转移酶(ASMT)和咖啡酸-O-甲基转移酶(CAMT)活性均低于根系.富硒酵母处理显著降低了叶片和根系的SNAT活性.

Understanding the spatial distribution of species is significant for the conservation management of the endangered tree species, Taxus wallichiana var. mairei . And this species attracts people's attention owing to its important biological compounds such as paclitaxel (taxol) used in many cancer therapies. Here, the potential geographical distribution of the maire yew was analyzed by using the Maxent model from Mid-Holocene to the 2070s. The results indicated that the prediction performances were excellent with an area under curve of >0.9 for model training and testing. The most significant factors were precipitation of the driest month, annual precipitation, altitude and min temperature of the coldest month, with thresholds of 22.3 to 122.5 mm, 1,100 to 2,100 mm, 50 to 1,100 m and -2 to 4 ℃ , respectively, indicating that the maire yew seems to prefer warm and humid environment. The suitable areas of the maire yew in China are mainly distributed on southwestern, central, southern and eastern China from Mid-Holocene to the 2070s. However, proportion of the suitable habitat is predicted to be increasing gradually from Mid-Holocene to the 2070s. Most importantly, western Henan, southern Shaanxi, eastern Sichuan and the border of Yunnan and Guizhou provinces etc. were predicted to be new suitable centralized areas for its introduction and cultivation. Thus, the study should be a useful guide for management decisions of the species.

以"青脆"李为试验材料,根施4种价态的硒,研究其对李子幼苗生长、根系谷胱甘肽氧化循环以及抗氧化系统的影响,以期为富硒李发展提供参考依据.结果表明:不同价态硒对幼苗生长产生不同的影响,其中Se6+和Se4+显著促进了株高和直径.4种价态硒处理显著提高了根系丙二醛(MDA)含量,也显著提高了过氧化氢酶(CAT)和谷胱甘肽转移酶(GST)活性.Se6+处理下根系总抗氧化能力(AOC)最高,其次为Se0,Se2-和Se4+最低.4种价态硒均显著提高了根系还原型谷胱甘肽(GSH)、氧化型谷胱甘肽(GSSG)和GSH+GSSG含量、GSH/GSSG比值,以及谷胱甘肽还原酶(GR)和谷胱甘肽过氧化物酶(GPX)活性,但4种价态硒处理间未存在显著水平.总之,硒对根系GSH氧化循环有促进作用,但不存在明显的价态差异,而不同价态硒对根系抗氧化系统和幼苗生长存在价态上差异.Se6+在促进生长和抗氧化能力表现较好.

利用高通量测序对复配小曲清香型白酒酿造过程中微生物多样性及变化规律进行比较分析.结果表明,样品中共检测出35个门、378个属的细菌和4个门、38个属的真菌.优势菌门为厚壁菌门(Firmicutes)、变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)、子囊菌门(Ascomycota)和毛霉菌门(Mucoromycota).小曲白酒发酵过程中菌群结构不断发生变化,发酵前2?d细菌优势菌属为不明立克次体菌属(unidentified?Rickettsiales),发酵3?d后的绝对优势菌属为乳杆菌属(Lactobacillus)直至发酵结束.真菌优势菌属为伊萨酵母属(Issatchenkia)、根霉属(Rhizopus)、酵母菌属(Saccharomyces)和曲霉属(Aspergilus).随着发酵时间延长,根霉属和曲霉属相对丰度都在降低,而酵母菌属在发酵第2天后其相对丰度在不断提高并成为优势菌.本研究揭示了复配小曲白酒酿造发酵过程中微生物群落多样性及变化规律,为复配小曲白酒的生产提供理论支持.

The interaction of selenium (Se) and melatonin in plants is unclear. The aim of this study was to investigate the effects of Se root application on melatonin synthesis in sweet cherry. Cultivar ‘Russia No.8’ was treated with Se6+ (sodium selenate) or Se2− (selenomethionine). Parameters of plant antioxidant levels, growth parameters, and the synthesis enzyme activities and precursor contents of melatonin were measured. Results showed that the plant benefited more from Se6+ than Se2− and were not exposed to Se stress by four low doses of Se6+ (0, 0.2, 0.4, and 0.6 mg kg−1) treatments for 40 days. The trends of tryptophan hydroxylase (TPH), tryptamine-5-hydroxylas, and arylalkylamine N-acetyltransferase activities were similar, while tryptophan decarboxylase (TDC), serotonin-N-acetyltransferase (SNAT), caffeic acid-O-methyltransferase, and N-acetyl-5-hydroxytryptamine-O-methyltransferase (ASMT) activities were different in leaves and roots following different Se6+ treatments. Tryptophan and 5-hydorxy tryptophan contents were not influenced by the Se6+ dose. Tryptamine was not detected in leaves or roots, but the serotonin content was increased by Se6+ treatment. The leaf content of N-acetyl-5-tryptophan was not influenced, but its synthesis was significantly induced in roots as the Se dose increased. Melatonin synthesis in leaves and roots was increased only following treatment with 96 mg Se6+. The leaf Se and melatonin content showed no significant linear relationship in the ‘Russia No.8’ cultivar. Treatment with Se2− increased the enzyme levels of TDC, TPH, SNAT, and ASMT more significantly than Se6+. Overall, lower doses of Se6+ did not significantly induce melatonin synthesis, but the relationship between melatonin and Se2− merits further attention.

以重庆地区种植的美早、红灯、早大果和萨米脱4个品种以及来源于山东种植的美早果实为试验材料,分析了果实的横纵径、果重、核重、果肉重、横纵比、可食率以及可溶性固形物含量、可溶性糖含量、可滴定酸含量、糖酸比等参数.结果表明,重庆地区的红灯与美早果实品质较好;重庆地区种植的美早外观品质优于山东地区,而在内在品质上尤其是可溶性固形物含量、可滴定酸含量明显低于山东地区.因此,重庆地区甜樱桃品种引进和选育应侧重果实的内在品质.

Pregnancy-associated glycoproteins (PAGs) are widely used as powerful markers for early pregnancy diagnosis in livestock. To improve expression efficiency of recombinant ovine pregnancy-associated glycoprotein-7 (ovPAG-7) in HEK293 cells, the codon usage bias of the ovPAG-7 gene was analyzed using bioinformatic approaches, after which the DNA sequence encoding ovPAG-7 was designed, synthesized, and expressed in HEK293. The structure and function of ovPAG-7 were predicted using bioinformatics software and online databases. The results showed that the effective number of codons (NEC) of the ovPAG-7 gene was 56.82, indicating that the ovPAG-7 gene was weakly biased. ovPAG-7 gene had 26 biased codons (relative synonymous codon usage (RSCU) > 1), 15 of which were biased towards G/C at the third position. After codon optimization, the codon adaptation index of the ovPAG-7 gene increased from 0.74 to 0.96, and its GC content changed from 46.6 to 58.6%. The amino acid sequence encoded by the optimized gene was entirely consistent with those published in Gen Bank. Western blot analysis indicated that the recombinant ovPAG-7 protein with a relative molecular mass of 48 kDa was successfully expressed in HEK293 cells. The bioinformatics prediction results showed that ovPAG-7 protein contained 3 N-glycosylation sites, 13 B-cell epitopes, and a signal peptide consisting of 15 amino acids at the N terminus. The secondary structure of the ovPAG-7 protein was predicted to consist of random coils (46.85%), extended strands (32.05%), α-helices (16.16%), and β-turns (4.93%). This study provided a tool for the screening of monoclonal antibodies and functional research on ovPAG-7.