As an alternative of traditional extraction method, pressurized solvent extraction (PSE) was applied for two flavanoids extraction from Acanthopanax senticosus (Rupr. et Maxim) leaves. The operational parameters of PSE, such as temperature, pressure, extraction time and cycles were optimized by orthogonal array design in order to obtain the highest extraction efficiency, and the contents of two major flavanoids in Acanthopanax senticosus (Rupr. et Maxim) leaves were determined simultaneously by HPCE method. The optimized results employed temperature 100°C, pressure 100 bar, extracting for 5 min 3 times to extract the target compounds completely. Compared with ultrasonic extraction, the PSE of flavanoids from Acanthopanax senticosus (Rupr. et Maxim) leaves is efficient, less extraction time, less solvent and no noise pollution and so on.

OBJECTIVE: To investigate the endophytic fungi from Schisandra chinensis(Turcz.)Baill. and their secondary metabolites. METHODS: Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Bacillus pumilus, Pseudomonas aeruginosa, Listeria monocytogenes, Klebsiella penumoniae, Acinetobacter baumannii, Streptococcus pyogenes, Enterococcus faecium, Enterococcus facalis and Candida albicans were selected as the indicators for human pathogenic microbes. Antimicrobial tests of the isolated endophytic fungi and their secondary metabolites were carried out by colony disc method and paper disc diffusion method. RESULTS: Among 36 strains of endophytic fungi isolated from Schisandra chinensis(Turcz.)Baill., 33 strains (91.67% of the total strains isolated) showed the different antimicrobial activity against 12 human pathogenic microbes, and the secondary metabolites of 30 strains at least (83.33% of the total strains isolated) presented the antimicrobial activity against 12 human pathogenic microbes. CONCLUSION: Diverse antimicrobial natural products are existed in endophytic fungi and their secondary metabolites from Schisandra chinensis(Turcz.)Baill., which can be the resources for new antimicrobial products.