ObjectiveTo analyze the status quo and balanced development of major chronic disease service provision capabilities in the Yangtze River Delta region of China, and to provide a reference for promoting high-quality and balanced development of major chronic disease prevention and control capabilities in the region. MethodsWe retrieved documents on prevention and control of major chronic diseases published by local administrations in the Yangtze River Delta region (including Shanghai municipality and three provinces of Jiangsu, Zhejiang and Anhui) during the period from January 2009 through December 2019. Taking a page from service provision elements for an appropriate public health system, service coverage rate (SCR) and assessable service coverage rate (ASCR) were constructed for quantitative analysis on major chronic disease service provision capability of the municipality and the three provinces in the region. Coupling coordination degree model was adopted to assess the balance of the service provision capacity development in the Yangtze River Delta region. ResultsThe overall SCR and ASCR of major chronic disease service provision for the Yangtze River Delta region increased from 21.2% and 10.0% in 2009 to 82.8% and 51.5% in 2019. In the year of 2019, the prevention level-specific SCR and ASCR were the highest for tertiary prevention; the administrative division-specific SCR of tertiary prevention was the highest (96.0%) for Shanghai municipality and the lowest (71.0%) for Anhui province, while, the administrative division-specific ASCR of tertiary prevention was the highest (71.0%) for Jiangsu province and the lowest (33.0%) for Zhejiang province. The overall index value for coupling coordination degree of SCR and ASCR of the Yangtze River Delta region increased from 38.97 and 11.58 in 2009 to 90. 73 and 70.72 in 2019; the overall scores of 2019 for the balance of SCR of primary, secondary and tertiary prevention in the region were 9, 9 and 10, and the overall scores for the balance of ASCR were 7, 7 and 8 respectively. ConclusionIn the Yangtze River Delta region during the period of 2009 – 2019, the major chronic disease service provision capacity and the balance for the capacity development increased yearly; the service provision capacity was higher for tertiary prevention than that for primary and secondary prevention and the development balance was higher for SCR than that for ASCR.

Osteoporosis is an age-related systemic bone disease that places a heavy burden on patients and society. In this study, we aimed to investigate the effects of naringin (NAR) on the osteogenic differentiation of human adipose-derived stromal cells (ADSCs). The results demonstrated that NAR pretreatment effectively abated H2O2-induced cell death and ROS accumulation in ADSCs undergoing osteogenic differentiation (ADSCs-OD). In addition, we also observed that the impaired extracellular matrix mineralization and ALP activity in H2O2-stimulated ADSCs-OD were notably rescued by NAR pretreatment. Moreover, the effects of H2O2 exposure on Wnt/β-catenin signaling in ADSCs-OD were largely reversed by NAR pretreatment. Collectively, our findings indicated that NAR could protect ADSCs-OD against H2O2-inhibited osteogenic differentiation.

目的 探讨茶多酚(Epigallocatechin-3-gallate,EGCG)对人脂肪间充质干细胞(human adipose-derived mesenchy-mal stem cells,hADSCs)成骨分化的影响.方法 利用胶原酶消化法和贴壁筛选法从人脂肪组织中分离、培养及扩增hADSCs,倒置显微镜下观察各代hADSCs的形态学特点;利用流式细胞术检测各代hADSCs免疫学表型;取P3代细胞进行成骨诱导分化,实验分三组,即未诱导组、常规成骨诱导组与EGCG组(常规成骨诱导+5μmol/L EGCG),14 d后,镜下观察细胞形态学改变及碱性磷酸酶(ALP)染色.结果 体外分离、培养的hADSCs形态均一;流式细胞术结果显示hADSCs具备间充质干细胞的免疫学表型,即CD44、CD73、CD105阳性;成骨诱导14 d后部分细胞由长梭形变成多角形,细胞呈现聚集趋势;ALP染色显示EGCG组呈强阳性.结论 成功的从脂肪组织中分离培养出了hADSCs,EGCG能加强其成骨分化能力,这将为骨质疏松症的临床药物开发提供新的思路,亦为组织工程骨的构建提供丰富可靠的种子细胞来源.

中药骨碎补应用于骨质疏松症的治疗在中国已经延续了上千年,疗效显著.柚皮苷作为骨碎补的主要成分之一已被众多学者应用于骨质疏松症(osteoporosis,OP)的防治研究中.氧化应激(oxidative stress,OS)与骨质疏松症之间存在着千丝万缕的联系,大量学者研究发现柚皮苷在抗氧化方面存在着卓绝的功效.柚皮苷主要通过多种信号通路发挥着抗氧化效用,促进成骨分化特异性基因的转录,从而诱导干细胞成骨分化.