Objective To establish a SOI model of human renal carcinoma and a high metastatic cell subline. Methods A human renal cell line RCC-9863 has been established by inoculating a human renal tumor tissue into nude mice s. c.. When RCC-9863 passaged for 20 times, the tissue from the same xemotransplant tumor were used to construct SOI model. Cultured the metastatic tissue in vitro, the tumor cell suspension was then injected orthotopically, The metastatic tissue obtained underwent the same procedure again. At last, the metastatic tumor was cultured in vitro and cloned. Results 15 days later, a tumor mass sized 1. 7 cm × 0. 6 cm in the nude mouse’s renal parenchyma was grown which lobulated, rude, and with multiply blood vessels and 55 days later later the mouse became moribund and metastases in the lungs were formed. The transplanted renal tumor in the SOI model grew fast and invasively and metastasized to lungs, lymphatic node and liver. A subline, MRCC, with metastatic ability to the lung was selected. Co

腺性膀胱炎的主要症状是尿频、尿急、尿痛,以往用电灼或药物治疗效果不佳.1997年至2001年我院用Nd∶YAG激光治疗87例,随访44例腺性膀胱炎患者,效果良好.

目的评价HLA氨基酸残基配型新标准在肾移植中的应用. 方法应用血清学和基因分型方法对134例肾移植供受者进行HLA-Ⅰ,Ⅱ类抗原分型,并比较氨基酸残基配型标准与传统的HLA六抗原无错配标准的关系. 结果 HLA氨基酸残基配型标准可以提高肾移植供受者的相配率,六抗原无错配率可由0.75%提高到2.24%,六抗原完全错配的几率由15.67%降至2.24%. 结论 HLA氨基酸残基配型标准是一种更为实用的同种异体移植配型策略.

目的探讨前列腺增生(BPH)的3种外科治疗方法血清IL--6和CRP水平的变化. 方法用前瞻、对比的临床实验,对117例分别进行开放性手术(OP)、经尿道气化电切术(TUEVAP)和组织间激光消融(ILC)的BPH患者,用酶联免疫吸附法(ELISA)检测其手术前后血清白细胞介素-6(IL-6)和C-反应蛋白(CRP)水平. 结果 3组患者血清IL-6和CRP水平术前无显著差异,但术后24 h明显高于术前(P<0.05),其中以开放手术组最高,TUEVAP组次之,ILC组最低;术后24 h血清IL-6水平与手术时间、术中出血量明显相关(r=0.69, P=0.0000),术后48 h血清CRP水平与手术时间相关(r=0.83, P=0.0000). 结论 3种外科治疗方法中,开放性手术对机体的创伤最为严重,急性时相反应最大,经尿道气化电切术次之,组织间激光消融则最小. 手术的操作时间和术中出血量是影响机体创伤程度的主要因素.

Objective To establish a strain of prostate tumor cell line and provide a useful animal model.Methods Prostate tumor specimens were inoculated into right posterior leg s.c of three nude mice.After two passages in the nude mouse,tumor tissue was cultured in vitro.Following the reference,the identification of cell line was fulfilled.Results All of the transplanted tumor grew in the nude mice and the growth was enhanced in the second passage in the nude mice.After the tissue cultured in vitro a stable growth prostate tumor cell line was obtained without change of morphological structure and differentiation.The mode rage of chromosome is 64 73 accounting for 80% and the cycle analysis showed G 1 was 53.2%,G 2 17.0% and S 1 29.8%.The doubling time was 16.7?h.The expression of PSA was negative.Conclusion The prostate tumor cell line PC 98106 was identified to primary cancer cell in biological characters,has been cultured in vitro continually for more than one year and its characters still remained.Therefore PC 98106 may be considered to be a stable cell line.

Objective To evaluate and compare the therapeutic effects of the open prostatectomy(OP)?transurethral electrovaporization ablation prostatectomy (TUEVAP) and interstitial laser coagulation(ILC) for the treatments of symptomatic BPH. Methods 282 symptomatic BPH patients randomly underwent OP (n=98)?TUEVAP(n=91) or ILC(n=93) respectively and the therapeutic effects were evaluated. Results There are significant improvements in symptoms and physical signs in each group after the procedure.The average catheterization time following OP?TUEVAP and ILC was 185 h?76 h and 362 h,the immediate morbidity rate being 28.9%?16.7%? 14.8 % and the retreatment rate 1.0%?6.6% and 16.1% respectively. OP group showed the longest average operative time and the most blood loss both of which were the least in ILC group. Conclusions OP is the most effective of the three procedures, but the trauma to the patient is the most.The therapeutic effect of TUEVAP is similar to OP's,with less injure.ILC is the easiest and safest procedure with the least side effects.

我们于1996年以来收治睾丸扭转患者14例，报告如下。 资料与方法本组14例。年龄5～20岁。左侧8例，右侧6例。发病到就诊时间4 h～2周。10例在外院曾按急性睾丸炎治疗1～2周。11例阴囊及睾丸肿胀、疼痛并向同侧腹股沟放散，睾丸上抬，触痛明显，与附睾分界不清，提睾反射消失，抬高睾丸时疼痛加剧；余3例阴囊空虚，腹股沟区可触及肿块并有压痛。就诊晚者血象偏高。14例均无发热，但伴有恶心、呕吐。彩色多普勒动态显像(CDFI)检查：2例发病早期就诊者示睾丸血流减慢，12例就诊晚者示睾丸明显增大，血流消失，睾丸坏死。

Objective:To investigate the effect of the diode laser therapy on serum total PSA,free PSA and free／total PSA ratio in pat ients with benign prostatic hyperplasia and to determine the reliability of fre e／total PSA ratio after such interventions.Methods:Using the Indigo 830 e diode laser system,72 cases of prostatic hyperplasia have been treated by transurethral prostatectemy with interstitial laser coagulation .Serum total PSA and free PSA levels were determined 1 hour before and 24 hours after laser therapy by using enzyme immunoassay.Preoperative and postoperativ e free and total PSA and free／total PSA ratio were statistically compared.Results:Although postoperative total PSA and free PSA in creased significantly compared with preoperative values (P＜0.01)，the diffe rence between preoperative and postoperative free／total PSA ratios was not stat istically significant (P＞0.05).Conclusion:Free ／total PSA ratio may be a reliable parameter in the early period after such int erventions as diode laser therapy.

AIM To discuss the expression and correlation of retinoblastoma susceptibility gene and proliferating cell nu clear antigen in the prostatic cancer. METHODS SABC immunohistochemistry was used to determinate Rb gene and PCNA in 36 samples of the prostatic cancer. The image pattern analyzer and the statistics methods were used to do the comparative analysis of Rb gene and PCNA in the different pathological grading and clinical phases of the prostatic cancer. RESULTS immunohistochemistry staining was Rb positive in 17 of 36 samples,rated 47.2%. The expression of Rb had significant differences in different pathological grading and clinical phases ( P 0.05). The expression of PCNA also had significant differences in different pathological grading and clinical phases ( P 0.05). The expression of PCNA was weak in the Rb positive expression samples and was strong in the Rb negative expression samples. There was a significant difference between Rb and PCNA expression ( P 0.05). CONCLUSION Rb is involved in the occurrence and development of the prostatic cancer. As for pathological mechanism, it is related to the abnormality of Rb and PCNA regulating path. The determination of Rb and PCNA can be an index for estimating the level of malignant and progression of the prostatic cancer.

目的探讨DNA倍性在肾细胞癌预后中的作用.方法运用流式细胞仪和图像分析技术对56例肾细胞癌标本进行DNA含量测定,按照肾癌的病理分级、临床分期及细胞类型进行DNA倍性的统计学分析.并对近二倍体和非整倍体肾癌患者的生存率进行统计学分析.结果肾癌的DNA非整倍体率与癌细胞的病理分级、临床分期呈递增关系,各病理分级、临床分期间的非整倍体率有显著性差异(P<0.05);而各病理细胞类型间的DNA非整倍体率无显著性差异.近二倍体肾癌患者生存率明显高于非整倍体肾癌患者(P<0.01).结论 DNA倍性与肾癌预后相关,综合病理分级、临床分期和DNA倍性可对肾癌的恶性程度及预后作出更准确的判断.

Objective To probe into the minimal invasive prostatectomy. Methods 70 cases of benign prostatic hyperplasia(BPH), the age from 65 to 91 years old, of high risk have been treated by transurethral prostatectomy with indigo 830e interstitial laser coagulation(ILC), most of the patients with one or two kinds of chronic disease such as coronary heart disease, chronic bronchitis, pulmonary emphysema, chronic renal insufficiency, diabetes, sequelae of cerebral hemorrhage and cerebral infarction. The patients have been followed up for 6-12 months. The treatment effect was evaluated by international prostate symptom score(IPSS), quality of living(QOL), maximal flow rate (Qmax) and residual urine. Results At 6 months after therapy, the IPSS score was 6.2±2.2, the QOL score was 2.0±2.2, Qmax was 17.2 ml/s±2.1 ml/s and residual urine 10.2 ml±2.4 ml. Conclusions The benefits of the diode laser treatment for BPH were less damage, high security and less complication. the best result could be achieved in 2-3 months after the procedure and the effective rate reaches 100%.

0 引言 腺性膀胱炎(CG)是一种膀胱粘膜增生性病变,自1899年Stoerck等首次报告以来,引起泌尿外科和病理科医师的重视. 近年来的报告显示发病率呈上升趋势,由于腺性膀胱炎具有特殊的病理过程和临床特点,临床上治疗较为棘手. 我院1994-01/1999-05共收治83例,取得满意疗效.

0 引言 尖锐湿疣是常见的性传播疾病,由人类乳头瘤病毒(HPV)感染所致,鬼臼毒素是治疗该病的有效药物,1990年世界卫生组织推荐用5 g.L-1鬼臼毒素酊为治疗尖锐湿疣的一线药物. 尤脱欣处方为5 g.L-1鬼臼毒素酊剂,为了减轻对皮肤的刺激性,增加药物粘稠度,便于涂药,我们研制了优克星(5 g.L-1鬼臼毒素凝胶剂). 自1997-09/1999-06共配制11批次(1600余瓶),制备工艺可行,质量稳定,经临床应用观察疗效良好,涂药较方便,局部刺激性较轻.

Objective Discuss whether there are new hypertension following extracorporeal shock wave lithotripsy(ESWL) and its possible reason. Methods Determinate the activity level of Plasma Renin(PR)－ Angiotension II(AII), Prostacyclin(PGI2) and Thromboxane A2(TXA2).Analysis the relationship between hypertension and shock wave. Results The levels of PR, AII and TXA2 of the patients with hypertension after ESWL were significantly higher than that of the patients with normotension. The levels of PGI2 were just contraverse. It was showed by statistics that there is a significantly relationship between the hypertension and shock wave. Conclusion The possible reason is that injure of the kidney by high energy shock wave results in ischemia in the kidney and irritates the kidney to release more renin and AII . Meanwhile, increased concentration of TXA2 and decreased PGI2 make the vessle walls more sensitive to AII.

0 引言睾丸扭转是一种比较少见的阴囊急症, 由于此病缺乏特异性,患者又多为儿童,病史叙述不清,若对其认识不足,极易误诊为急性附睾炎等而延误治疗,失去挽救睾丸的机会. 我院1996年以来共收治12例,报告如下:

目的研究激光治疗前列腺增生症患者血清总前列腺特异性抗原(PSA),游离PSA和游离/总PSA比值的变化,确定游离/总PSA比值的可靠性.方法应用830e半导体激光仪对100例前列腺增生症患者行组织内激光凝固治疗, 用酶联免疫法于术前1 h和术后24 h检测患者血清总PSA和游离PSA值,并计算游离/总PSA比值.结果术后总PSA和游离PSA值与术前比均明显升高(P<0.001和 P<0.05), 游离/总PSA比值术前术后无显著差异(P=0.103).结论游离/总PSA比值可作为激光治疗前列腺增生症可靠指标.

目的探讨保留肾组织手术治疗肾肿瘤的实际应用价值及适应征. 方法 1997-06/1998-12采用保留肾组织手术方法治疗肾肿瘤11例. 11例中恶性肿瘤7例,良性肿瘤4例. 结果平均随访12.5 mo,术后均未发现有局部肿瘤复发及远处转移,肾功能正常. 结论保留肾组织手术具有与根治性肾切除术同样良好的预后效果,可用于小肾癌和肾良性肿瘤的治疗.

我院于1999年4月至2000年4月采用美国强生公司最新型产品Indigo830E增强型组织内凝固激光治疗仪治疗BPH患者100例。报道如下。临床资料：BPH病人100例，年龄65～92岁，平均81岁，均有典型的前列腺增生症临床表现。其中急性尿潴留65例，经B超检查，计算出前列腺体积30～120ml。尿流动力学检查患者尿流率曲线上升缓慢。表现TQmax延长。

0 引言 男性尿道癌非常罕见,且以鳞癌占绝大多数,移形细胞癌约占1 5%,且可伴发膀胱、输尿管或肾盂癌[1]. 我院1989-11/1999-11共收治尿道移行细胞癌11例,报告如下.