Objective: Bone marrow stromal stem cells（BMSCs） of SD rats were cultured and identified by the method of whole bone marrow culture in vitro. Methods: Mouse bone marrow stromal stem cells were isolated and enlarged by the whole bone marrow adherent method, and the expressions of CD29, CD34 and CD45 on the cell surface were detected by flow cytometry. Results: The whole bone marrow culture method can rapidly and effectively culture and obtain a large number of bone marrow stromal stem cells. The positive rate of CD29, CD34 and CD45 were 99.0%, 1.5% and 0.8%respectively. Conclusion: The whole bone marrow adherent culture is a simple, efficient and economical way to obtain bone marrow stromal stem cells, and can obtain relatively pure BMSCs.

目的:研究和探讨整合素β1在转化生长因子(TGF)诱导BMSCs分化为软骨细胞过程中的作用.方法:全骨髓法体外培养大鼠骨髓基质干细胞(BMSCs),TGF-β3促进和诱导BMSCs的软骨分化、抗整合素β1单克隆抗体抑制整合素的表达.实验分为对照组、诱导组和抑制组,BMSCs培养7d和14d后,分别采用免疫细胞化学(SABC法)检测Ⅱ型胶原(col-Ⅱ),甲苯胺蓝染色检测蛋白多糖的表达情况,Western Blot检测型胶原蛋白和相应整合素β1蛋白含量的表达.结果:培养7d和14d后,诱导组col-Ⅱ和蛋白多糖的表达均呈阳性,细胞形态由原来的长形或长梭形变短变圆,而对照组和抑制组均为阴性.诱导组col-Ⅱ和相应整合素β1蛋白的表达量均增多,而抑制组则较对照组减少,但两组的col-Ⅱ表达均为阴性.结论:TGF-β3能上调整合素受体的表达,促进BMSCs的软骨分化;抗整合素β1的单克隆抗体可以下调整合素β1的表达,抑制BMSCs的软骨分化.

用FPGA"片上系统"结构取代目前心电监护仪所采用的DSP和MCU或ARM微处理器双CPU结构,构建ECG信号处理模块,实现对ECG信号的数字处理和控制.模块设计采用软硬件协同设计的思想,在QuartusⅡ开发环境中,通过硬件描述语言(VHDL)或采用SOPC定制IP核技术完成AD控制器、心率处理器、FIR滤波器以及寄存器编码等子模块的设计,实现相关逻辑功能.该模块研制周期短,成本低,精度高、体积小、功耗低,具有较好的实用性和可扩展性,特别适应可穿戴设备和移动医疗的需要.