In the conditions of experi mental chronic gastritis and duodenitis reproduction, carried out among Wistar line 24 rats, was investigated effect of the nitrogen oxide stable metabolites content in the gums homogenates on a lamina propria structure and function. Results of our research have been shown, that level of nitrogen oxide stable metabolites in the rats’ gums tissues homogenates with gastritis and duodenitis decreased in three times, compared with a same period at the intact animals, which indicated about depletion of a nitrogen oxide depot at the given disease. It was revealed a significant decrease typical for the protein and glycoprotein content in the animals gums tissues at the experimental group, which indicated about the catabolic processes predominance. Amount of hexosamines in the gums was significantly increased, which was correlated with a morphological picture (gums lamina propria impregnation with the blood plasma proteins), testified about a change in the vascular wall permeability and microcirculation disruption. In the animals from experimental group was reduced a height of gums papillae connective tissue to 27.18±1.86 x 10-6 m against 56.93±2.64 x 10-6 m in the rats from control group. In the gums lamina propria defines phenomenon of the papillary and reticular layer fibrosis. After medicamentous correction, carried out with an antioxidant and the nitrogen oxide donor was observed repairing structure of the gums lamina propria on a background of increasing microvasculature area. It was determined significantly increased protein and glycoprotein content in the gums tissues with increased levels of nitrogen oxide metabolites.
Ряд наказів Міністерства освіти і науки України стимулюють вчених публікувати свої статті за кордоном, але не вказують критерії вибору рекомендованих журналів. У результаті автори обирають найпростіший спосіб і розміщують свої роботи в зарубіжних журналах низької якості. Більше того, з моменту вступу цих наказів у силу було створено велику кількість несправжніх журналів. У цій статті підкреслюється важливість реєстрації журналу в наукометричних базах даних для міжнародних і регіональних рейтингів університетів. Також приділяється увага необхідності перевірки авторами якості журналу і його надійності перед відправкою рукописів для публікації. Для вирішення цих проблем ми пропонуємо вказувати URL посилання на кожну статтю в річному звіті з наукової діяльності вчених, кафедр і організацій. Це допоможе в перевірці якості журналу, а також зверне увагу авторів на перевірку існування веб-сайту журналу перед подачею рукописів. Важливо підкреслити, що журнали без веб-сайтів не впливають на рейтинг організацій у жодній з наукометричних баз. Якщо подібне посилання відсутнє, то така стаття не повинна зараховуватися до рейтингу. Додатковий спосіб полягає в створенні адекватного наукового рейтингу. Наприклад, статті, опубліковані в провідних наукометричних базах (Scopus і Web of Science), повинні оцінюватися в два рази (і більше) вище, ніж всі інші статті. Роботи, опубліковані в інших наукометричних базах (наприклад, РІНЦ), також повинні оцінюватися вище, ніж всі інші. Крім того, із вченими слід проводити роз’яснювальну роботу для допомоги у виборі оптимального видання для розміщення своїх публікацій.
Aim of the study: to determine relationship between antiamnesic properties of piracetam and phenylpiracetam and their influence on the processes of proteins oxidative modification and production of nitric oxide in brain cortex of rats with alloxan-induced hyperglycemia. Diabetes mellitus was induced in rats by a single administration of alloxan monohydrate (150 mg/kg). Antiamnesic activity of piracetam (500 mg/kg) and phenylpiracetam (100 mg/kg) was estimated in conditions of hyoscine-induced amnesia (0,8 mg/kg) in the passive avoidance test. The drugs were administered intragastrically once per day during 20 days from the 11th day after alloxan injection. On the 30th day the level of aldehydephenylhydrazones (APH) and ketonphenylhydrazones (KPH), total level of nitrite and nitrate (NOx) in homogenates of cerebral cortex were determined by spectrophotometric assay. It has been found that long-term hyperglycemia potentiates the amnesic properties of hyoscine and is accompanied by increased intensity of proteins oxidative modification and synthesis of nitric oxide in the brain cortex. Phenylpiracetam possesses greater antiamnesic potential as compared to piracetam. Also it significantly reduces the amount of early (APH) and late (KPH) markers of protein molecules degradation and decreases the level of nitric oxide stable metabolites. Conclusion. These results reveal new mechanisms of neuroprotective properties of the studied drugs, particularly in diabetes mellitus.
In rats with chronic alloxan-induced hyperglycemia, pramiracetam and phenylpiracetam (but not piracetam) had a strong antiaggregant effect that was mediated by various mechanisms of platelet aggregation modulation. The effect of pramiracetam is mainly realized via the inhibition of thromboxane A 2 metabolism, while activity of phenylpiracetam is primarily associated with a normalizing effect on function of constitutive NO synthase in platelets and vascular endothelium.
It has been established that prolonged alloxan-induced hyperglycemia in rats potentiates amnesic properties of scopolamine hydrobromide. It was characterized by shortening of the latent period by 44% (p<0,01) and by 47,7% (p<0,05) after 24 hours and on the 20th day of conditioned passive avoidance test. This effect was accompanied by increase in oxidative modification of proteins and nitric oxide synthesis in the cerebral cortex. Along with this, a significant enhancement of ADP- and collagen-induced platelet aggregation was observed. These processes may play the leading role in the development of cognitive deficit in diabetes. Meanwhile, co-administration of piracetam with acetylsalicylic acid was accompanied by an expressed antiamnetic potential - the reduction of early markers of proteins degradation (aldehydephenylhydrazones, APH) by 21,7% (p<0,05) and late markers of proteins degradation (ketonephenylhydrazones, KPH) by 23,8% (p<0,001) was noted. This combination was 15,7% (p<0,05) more active than piracetam according to the effect upon KPH. NO2-/NO3- level was also decreased by 30,3% (p<0,05) in comparison with alloxan-diabetic rats. The significant anti-platelet effect was observed: degree of collagen-induced platelet aggregation was reduced by 56,8% (p<0,01), ADP (5 μmol/l)-induced - by 31,7% (p<0,01), ADP (20 μmol/l)-induced - by 47,3% (p<0,01) as compared to the hyperglycemic rats. Such an increase in nootropic activity of piracetam may be assumed to be directly related to the ability of acetylsalicylic acid to improve microcirculation in the ischemic areas of the brain in diabetes and probably to its neuroprotective potential.
The aim of the study was to investigate the antithrombotic efficiency, safety and tolerance of Lospirin tm in patients with coronary artery disease. The study involved 50 patients (32 men and 18 women) aged 40-65 years with disease duration from 3 to 27 years, receiving daily one tablet of Lospirin tm (75 mg) for 28 days as an antiplatelet agent in addition to basic therapy. General clinical data, hemodynamic parameters, laboratory results of blood tests, urine and agregant state of blood plasma were assessed. During the period of drug application side effects/reactions were not registered. Tolerance of Lospirin tm was satisfactory in 100% of patients. Drug intake for 28 days gave good antiplatelet results in 90% of patients with coronary artery disease.
The aim of the study was to investigate the antithrombotic efficiency, safety and tolerance of Lospirintm in patients with coronary artery disease. The study involved 50 patients (32 men and 18 women) aged 40-65 years with disease duration from 3 to 27 years, receiving daily one tablet of Lospirintm (75 mg) for 28 days as an antiplatelet agent in addition to basic therapy. General clinical data, hemodynamic parameters, laboratory results of blood tests, urine and agregant state of blood plasma were assessed. During the period of drug application side effects/reactions were not registered. Tolerance of Lospirintm was satisfactory in 100% of patients. Drug intake for 28 days gave good antiplatelet results in 90% of patients with coronary artery disease.
The effects of nootropic drugs (noopept, pentoxifylline, piracetam, pramiracetam, Ginkgo biloba extract, entrop, cerebrocurin and citicoline) on platelet aggregation in rats with experimental diabetes have been studied. It is established that all these drugs exhibit an inhibitory action of various degrees against platelet hyperreactivity under conditions of chronic hyperglycemia. The maximum universality of the antiaggregatory action is characteristic of pramiracetam, entrop and Ginkgo biloba extract.
The effects of nootropic drugs (noopept, pentoxifylline, piracetam, pramiracetam, Ginkgo biloba extract, entrop, cerebrocurin and citicoline) on platelet aggregation in rats with experimental diabetes have been studied. It is established that all these drugs exhibit an inhibitory action of various degrees against platelet hyperreactivity under conditions of chronic hyperglycemia. The maximum universality of the antiaggregatory action is characteristic of pramiracetam, entrop and Ginkgo biloba extract.
