The metabolic features of the rat liver were studied in artificial homeostasis conditions, using an isolated perfused organ as a model. The metabolism of the liver isolated from an intact rat and perfused with a normobaric hypoxic medium was compared with that of a liver that was isolated from a rat preliminarily kept in a chamber to simulate hypoxia of the total body and perfused using a medium with a normal oxygen content. The functional activity of the liver was assessed by portal pressure; oxygen consumption; and carbon dioxide gas, urea, glucose, and lactate contents in the perfusion medium. Metabolic changes in the perfused liver during oxygen deficiency became detectable at the same time point after exposure regardless of the method used to experimentally simulate hypoxia. This finding directly points to the metabolic autonomy of the liver.

It is shown that the transport of oxygen through the surface of the isolated perfused rat liver is an energy-dependent process that requires the energy of ATP hydrolysis.

The effect of the perfusion conditions (duration of the preparatory period, noradrenaline and lactic acid) on the characteristics of isolated rat liver functioning is determined in the present research.

The research results on estimation of nitrogen-containing and carbohydrate compounds metabolism in the isolated perfused rat liver undergoing the hypoxic conditions simulated by cobalt chloride are presented in the article.

It is convenient to study liver function and metabolism on the model of isolated perfused organ. The results of the present study indicate that viability and metabolic activity of the organ largely depend on the composition of the medium. Under conditions of isolated perfusion, the known pathway of oxygen transport through capillary filtration is supplemented by oxygen delivery to cells through the organ surface making an important contribution to liver oxygenation.

The nature of the chemical bond of complexes of iron and cobalt porphyrinates with ligands is studied by the quantum-chemical method in the Hartree–Fock self-consistent field approximation using the 3-21G basis set. The addition of oxygen molecule to the MP and MPIm complexes (M = Fe, Co; Im is imidazole) is established to be more favorable than water addition. However, imidazole, which is the second ligand in the MPImO2 and MPImH2O complexes (M = Fe, Co), increases the M–O2 and M–H2O binding energies for iron, but decreases them for cobalt. The Co atom is bound with the porphyrin ring more strongly than the iron atom due to the larger total overlap of the atomic orbitals. The calculations of the binding energy in the complexes demonstrate similar changes in the structures of the spatial conformation of the deoxy form (FeP + H2O) of iron porphyrinate and the oxy form (CoP + O2) of cobalt porphyrinate. This is an argument in favor of the hypothesis of hemoprotein sensor of partial oxygen stress in tissues.

Haemopoietic stem cells content and proliferative activity were studied in the bone marrow of female F1 (CBA x C57Bl6) mice after single (50 mg/kg) and chronic (0.5 mg/kg daily for 7 days) serotonin (S) injections. It is shown that 9-day and 12-day COEs contents in the bone marrow of experimental mice has been increasing for 24 h after single S injection. After chronic S injections twofold increase of 12-day COEs is observed without any increasing of 9-day COEs. Total myelokaryocyte number is increased too. The study of proliferative status by in vitro incubation of bone marrow cells with ARA-C has shown that the numbers of 9-day and 12-day COEs in S-phase have increased both after single and chronic S injections. Possible mechanisms of stimulating effect of S on bone marrow stem cells are discussed.