Cytokine secretion, apoptosis and production of active oxygen forms in cell cultures for their in vitro contact with implants carrying microarc, magnetron or ablation calcium-phosphate coatings have been studied in the work. Mononuclear blood leukocytes have been established to be first of all cells regulating the acceptance/rejection of implants with different roughness. Implants with “smooth” calcium-phosphate coatings are less suitable for bone tissue reparative regeneration.

Cytokine secretion, apoptosis and production of active oxygen forms in cell cultures for their in vitro contact with implants carrying microarc, magnetron or ablation calcium-phosphate coatings have been studied in the work. Mononuclear blood leukocytes have been established to be first of all cells regulating the acceptance/rejection of implants with different roughness. Implants with “smooth” calcium-phosphate coatings are less suitable for bone tissue reparative regeneration.

We studied the in vitro apoptosis-inducing effect of recombinant TNF-α (rTNF-α) on blood lymphocytes from healthy donors. rTNF-α-induced apoptosis was accompanied by an increase in the number of cells with low mitochondrial transmembrane potential, increased intracellular content of reactive oxygen species, reduced content of Bcl-2, Bcl-xL, and Bax proteins, and elevated Bad content. The molecular mechanisms of these changes are discussed.

The effect of recombinant TNF-α on programmed death of donor lymphocytes was studied in vitro. The proapoptotic effect of this cytokine is realized through transcription factors and cell cycle inhibitors. Incubation of lymphocytes with recombinant TNF-α revealed increased levels of NF-kB and p21 and reducted content of nonphosphorylated p53.

The effects of recombinant IL-2 on apoptosis of lymphocytes of healthy donors were studied in in vitro experiments. It was shown that the inductive and inhibitory effects of IL-2 on apoptotic process depend on the dose of the cytokine and cell microenvironmental conditions. Culturing of lymphocytes with recombinant IL-2 increases the percent of cells with reduced transmembrane potential, reduces the content of intracellular proteins Bcl-2, Bcl-X L и Bax, and increases the level of Bad. The proapoptotic effect of this cytokine is realized with participation of nuclear transcription factor NF-кB and transcription factor P53.

Research of influence of recombinant tumor necrosis factor alpha, interleukin-2 and interleukin-4 in vitro on the apoptosis of lymphocytes is performed. It is revealed that a proapoptotic effect of these cytokines is dose-dependent and is realized with the assistance of reactive oxygen species and mitochondrias.