Introduction. Mechanisms of change in vascular reactivity and synthetic endothelial function remain controversial in thyroid pathology. Controlled hemostasis in areas of vascular injury is one of the important functions of the endothelium. Lack of coordination between synthetic and vasomotor endothelial function may be a possible cause of increased tissue bleeding in the surgical treatment of toxic goiter. Methods. Endothelial function and guanylate cyclase activity of smooth muscle cells of the brachial artery (GCAM) were studied in a prospective observational study. 49 women aged 44 (33; 50) years were examined. Vasomotor function of the brachial artery in response to short-term ischemia (EDVD) and oral administration of nitroglycerin (ENVD), flow-induced production of endothelin-1, prostacyclin and nitric oxide were measured. The parameters were evaluated in hyperthyroidism, drug-treated hyperthyroidism and on the 4–5 day after thyroidectomy in postoperative hypothyroidism. Results. We have established the consistency of flow-induced production of nitric oxide and endothelin-1 (r = 0.78; p = 0.01) in toxic goiter. Paradoxical vasoconstriction in response to sublingual glyceryl trinitrate administration or short-term ischemia was associated with excessive level of endothelin-1 (> 0.36 fmol/ml; p = 0,05) and TSH suppression < 0.03 mIU/L (p = 0.003). The magnitude of the EDVD varied depending on the ENVD and flow-induced production of prostacyclin (R = 0.87; RI = 0.75; p = 0.00001). Deleting of the thyroid gland led to a decrease of EDVD and increase of GCAM. The relationship between the magnitude of variability of these parameters had a linear character (r = 0.66; p = 0.02). Findings. TSH suppression below 0.03 mIU/L in toxic goiter is accompanied with excessive synthetic endothelial function, which is implemented in a paradoxical vasoconstriction. Increased ability of the endothelium to synthesize prostacyclin may be a possible cause of increased tissue bleeding while removing toxic goiter.

The aim of the research: to assess the effect of antioxidant preparation “Mexidol” in vitro on the content of phosphatidylcholine and phosphatidylethanolamine in erythrocyte membrane in patients with ulcerative colitis at the acute stage. We examined 34 patients having hospital treatment on the account of ulcerative colitis at the acute stage and 30 clinically healthy people (control group). Using high-performance thin-layer chromatography, we studied the contents of phosphatidylcholine and phosphatidylethanolamine in erythrocyte membrane after their incubation in normal saline and in Mexidol solution. In comparison with clinically healthy people, patients with ulcerative colitis had significantly lower content of phosphatidylcholine in erythrocyte membrane and statistically significant increase in the levels of phosphatidylcholine and phosphatidylethanolamine as compared to their content at their incubation in normal saline. We determined different character of the relationship between these membrane phospholipids in compared groups.

The program of mathematical processing of the electrophoregramm of protein strips is suggested. It is intended for definition of weight of proteins in a researched sample and has the purpose to automatically establish coordinates of the maximal concentration of proteins in investigated strips and to calculate the area under their peak using the algorithm of integration with an adaptive choice of a step, after reading the scanned strip of electrophoresis with expansion (.pcx) and processing of the received digital matrix.

The aim of the work was to study the peculiarities of the influence of bloodfactors on the development of spherocytosis in patients with coronary disease and arterial hypertension. The study involved 30 patients with coronary disease and 24 patients arterial hypertension with the study of their clinical status and biochemical parameters of blood, hemostasis, blood cell analysis. It was found that the increase in the pool spherocytic cells in patients with arterial hypertension were associated with increased nitric oxide production, while in patients with coronary disease spherocytosis was due to an increase in the concentration of reactive oxygen metabolites.

This review presents the observation of the role of endothelial dysfunction in development of atherosclerosis, coronary disease and. hypertension. Among other causes of endothelial dysfunction the most significant are hemodynamic factors (wall transverse strain, transmural pressure), dyslipoproteinemia, hyperhomocysteinemia, hyperglycemia, free radical damage of endothelium. It is supposed that in development and. progressing of coronary disease more significant role may belong to endothelial dysfunction rather than to morphological vascular changes, predetermined by atherosclerosis. However, there is still no clear view of development of endothelial dysfunction and. mechanisms of development and. progressing of both isolated forms of coronary disease and arterial hypertension and their combination.

Detection of role of separate components of celluar transplant in restoration of injured organ is one of the key moments for understanding of mechanisms of pathologic process regress at the use of cell therapy. It was revealed that transplantation of entire cells, their nuclei and mitochondria had ambiguous effect on the course of experimental toxic hepatitis. It was showed that transplantation of nuclei caused greater integrity and adequacy of regenerative processes than transplantation of entire cells or their mitochondria had

Th e authors studied the impact of xenogeneic neonatal heart cells on ATPase activity and the level of ions in cardiac tissue of rats with adrenal damage. It was found that cells transplantation contributed to the preservation, restoration and early activation of ATPase, which resulted in the early stages of the experiment to a higher level involved in the process of adaptation of the ions: calcium, magnesium, potassium.

General principles of erythrocyte's cytoskeleton structure and structural and functional features of its membrane are observed in the article. Data about the role of different components of erythrocyte membrane in their interaction with extracellular matrix are given. Main typical breaks of structure and function of blood red cells at pathologies of different genesis are shown.

Cell transplantation in the prior suppression of protein synthesis in the body continued its protective effect, which may be associated with the direct inflow into the damaged organ of the protein components of the graft.