The development of disturbances in energy processes and damaging free radical reactions in various pathological processes, including cardiovascular diseases, are interrelated and lead to a significant deterioration in the course of diseases.Aim of the study. Research of the dynamics of malondialdehyde, creatine phosphate and lactate in the cardiac tissue of rats under experimental adrenaline stress and during its correction with neonatal, xenogenic, cardiac cells.Methods. The experiment was carried out on outbred male rats. Adrenaline damage to the heart was simulated by a single subcutaneous injection of 0.1% adrenaline solution at a dose of 0.5 mg per 100 g of body weight. The first group (37 rats) was injected subcutaneously with adrenaline, the second group (41 rats) – adrenaline and isolated heart cells of a newborn rabbit at a dose of 500 000. The third group included 6 healthy rats.Results. It was found that a spike in the level of malondialdehyde and, accordingly, the activation of free radical processes in adrenaline damage to the heart, occurred during the restructuring of the energy of the heart cell from intense glycolysis to the restoration of active mitochondrial ATP synthesis (which corresponded to the end of the depletion of lactate and creatine phosphate, and the beginning of the restoration of their content in heart cells). The dynamics of MDA sensitively reflected both the activity and the inhibition of oxidative processes in mitochondria, which manifested itself, respectively, both in the form of peaks and in a low level of MDA and corresponded to the interpretation of the dynamics of lactate and creatine phosphate.In the cardiac tissue of rats with transplantation of neonatal, xenogenic cardiac cells, the accumulation of lactate in the early stages of the experiment decreased, the subsequent depletion of the cellular reserves of creatine phosphate and lactate was inhibited, the period of inhibition (non-increase) in MDA was shorter, the subsequent increase in MDA was more moderate than in control animals.Conclusion. The data obtained indicate that neonatal cardiac cells are able to limit the disturbance of aerobic and of anaerobic processes in the heart tissue and promote the restoration of mitochondrial energy processes. Moreover, they contribute to a more efficient restoration of mitochondrial ATP synthesis, accompanied by a smaller burst of damaging free radical processes.

We present the results of comparative analysis of functional and morphological changes in the liver of animals with experimental CCl 4 -induced hepatitis under conditions of transplantation of neonatal liver cells and nuclei. It was found that transplantation of neonatal liver cell nuclei in acute toxic hepatitis provides better functional and structural state of the target organ.

nitroglycerin test. Results. In decompensated DM2 patients, even at rest, BA blood flow and elasticity were reduced. In Celermajer D.S. test, BA diameter increase was twice as low as in healthy individuals. Shear stress endothelial sensitivity in participants. In diabetic patients with carotid atherosclerosis, BA diameter increase in nitroglycerine test was significantly lower than in participants without peripheral artery atherosclerosis. Conclusion. In DM2 patients, atherogenic lipid metabolism disturbances resulted in reduced endothelial sensitivity to shear stress, and endothelial dysfunction in middle-size arteries. In inadequate hyperglycemia compensation, endothelial dysfunction was observed more often.

Detection of role of separate components of celluar transplant in restoration of injured organ is one of the key moments for understanding of mechanisms of pathologic process regress at the use of cell therapy. It was revealed that transplantation of entire cells, their nuclei and mitochondria had ambiguous effect on the course of experimental toxic hepatitis. It was showed that transplantation of nuclei caused greater integrity and adequacy of regenerative processes than transplantation of entire cells or their mitochondria had

The effects of transplantation of xenogenic neonatal heart cells on energy processes were studied in rat myocardium during the early period of epinephrine injury. Transplantation promoted a less pronounced ATP hydrolys to adenosine monophosphate and inorganic phosphate, a higher level of creatine phosphate, pyruvate, lactate, retention and activation of myocardial enzymes involved in energy metabolism.

We studied the effect of xenogenic neonatal cardiomyocytes on enzymatic processes in rat myocardium under conditions of epinephrine-induced damage. It was found that transplantation of the cell preparation restricts the zone of suppressed enzyme activity in the myocardium and promotes its recovery. Less pronounced metabolic disturbances corresponded to less pronounced morphological changes in the myocardium.

Th e rates of blood fl ow and functional state of endothelium vascular wall were examined prior to and following the ischemic test in patients with exertional angina of the II-III functional class and in the control group. Multiparameter statistical analysis allowed determining signifi cant parameters which distinguished the given groups of patients. It was stated that endothelium dysfunction in patients with angina of diff erent severity was determined with diff erent predictors. In addition, dysfunctional changes caused by the disruption of endothelium of vascular wall were revealed in patients with angina of III functional class.

After conducted investigations it was established, that degrees of manifestation of feedback on ischemic test in practically healthy people and patients with CHD with different degree of stenocardia differ significantly. It testifies to different functional state of endothelium. Obtained, data revealed, the most informative parameters which were characteristics of feedback on ischemic test in s

A rapid method for evaluation of the functional activity of the monooxygenase system of the liver has been developed based on the thin layer chromatography (TLC) determination of the antipyrtine content in the saliva. Advantages of the proposed TLC method are the simplicity of sample preparation for the analysis, economy, rapid processing, and reliability. The method can be used to assess the detoxicating function of liver under clinical and outpatient setting conditions.

There was detailed investigation of lipidic metabolism, changes which are on the whole characterized, as atherogenic and are observed, in the majority of patients with hypofunction of thyroid. It has been established that intensity of lipidic metabolism disturbances is a predictor of deviations of psycho-emotional and cognitive functions, at that these changes are found already at subclinical hypothyroidism

Detection of specific agents of fetal and neonatal cells determining the functional recovery of the injured organ is one of the key moments in cell transplantation. Currently much attention is given to cytoprotective features of heat shock proteins (HSPj. The best-studied today is the family of HSPW. Under the stress (oxidation, metabolic, etc.) the cellular nucleus, cytoplasm and membranes accumulate the denatured proteins. HSP binds with the denatured proteins and induces their unwrapping prevents from aggregation, retaining the protein conformation necessary for the renewal of its normal functional activity. Moreover, HSP70, like many other HSPs, is a potent inhibitor of programmed cell death. There is high probability that the positive effect of cellular transplantation in pathology is determined among other things by modulation of HSP70 sinthesis in recipients heart under transplantation of neonatal cells.

A rapid method for evaluation of the functional activity of the monooxygenase system of the liver has been developed based on TLC determination of the antipyrine content in saliva. Advantages of the proposed TLC method are the simplicity of sample preparation for the analysis, economy, rapid processing, and reliability. The method can be used to assess the detoxifying function of liver under clinical and outpatient conditions.

The authors consider the possible mechanisms of preconditional protection of the heart, and role of heat shock proteins in its formation. The use of metabolic adaptation phenomenon is discussed.