Objective To examine the expression of TLR2 in human peripheral blood mononuclear cells(PBMCs) stimulatedby Legionella.Methods Human peripheral blood mononuclear cells(POMCs) were treated with different concentrations of Legionella(2×106/ml,2×107/ml),and real timel reverse transcriptase PCR(RT-PCR) was used to detect TLR2 mRNA expression.Results Factorial analyses showed that leading effect of time had statistical significance(F=26.06,P0.05) and there was an interaction between the time and concentration(F=11.39,P0.05).In 24 hours,the expressions of TLR2 mRNA of PBMCs stimulated with 2×106/ml and 2×107/ml Legionella were obviously higher than TLR2 mRNA expression in control group.In 48 hours,there was no difference between stimulated group and control group.In 72 hours,the expressions of TLR2 mRNA of PBMCs stimulated with 2×106/ml and 2×107/ml Legionella,were obviously lower than TLR2 mRNA expression in the control group.When the PBMCs were stimulated at the level of 2×106/ml and 2×107/ml Legionella,the difference in the expression of TLR2 mRNA in each period was statistically significant.Conclusion Expression of TLR2 mRNA in human peripheral blood mononuclear cells with Legionella is correlated with the time and dosage in vitro.The research provides a reference for the time and dose in the study.

Objective To investigate the relationship between Toll-like receptor4(TLR4) gene(2244G→A) or(2299A→G) polymorphism and Legionella infection. Methods The 16Sr gene of Legionella in sputum samples was amplified with polymerase chain reaction(PCR) and the antigen of Legionella pneumophila serogroup 1 strain was detcted with enzyme immune assay(EIA) for pulmonary infection patients.Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) was used to analyze the TLR4(2244G→A) and(2299A→G) gene polymorphism in 25 Legionella infected patients,44 non-gram-negative bacterial infected patients,and 47 healthy individuals. Results There were significant differences among the three groups in TLR4(2244G→A) polymorphism(χ2=17.234,P=0.002;χ2=15.332,P0.001).There was a significant difference between healthy controls and other groups in TLR4(2244G→A) polymorphism(P0.05).There was no significant difference between Legionella infected patients and case controls(P0.05).There was no significant difference among the three groups in TLR4(2299A→G) polymorphism(P0.05). Conclusion TLR4(2244G→A) polymorphism is associated with the susceptibility of Legionella and other non-gram-negative bacterial infection.

Objective To discover differences in risk factors of community acquired pneumonia and hospital acquired pneumonia and to provide the scientific basis for public health emergency control.Methods Pneumonia cases were collected consecutively in the First Hospital of Shanxi Medical University from May 2008 to October 2009.A total of 244 cases of pneumonia were collected with 178 cases of community acquired pneumonia and 66 cases of hospital-acquired pneumonia.Then risk factors were compared between the two groups.Results Univariate analyses showed that the two groups were significantly different in gender,age,education level,underlying disease,passive smoking,and history of exposure to respiratory tract infection.But there was no significant difference in occupation,smoking,drinking,and travel history.Multivariate analyses showed that the difference was significant in age,education level,underlying disease,and geographic distribution.Conclusion There are different risk factors between community-acquired pneumonia and hospital-acquired pneumonia and control measures should be taken in different ways.