Genetic analysis in Caenorhabditis elegans has uncovered essential roles for DAF-16 in longevity, metabolism, and reproduction. The mammalian orthologs of DAF-16, the closely-related FOXO subclass of forkhead transcription factors (FKHR/FOXO1, FKHRL1/FOXO3a, and AFX/FOXO4), also have important roles in cell cycle arrest, apoptosis and stress responses in vitro, but their in vivo physiological roles are largely unknown. To elucidate their role in normal development and physiology, we disrupted each of the Foxo genes in mice. Foxo1-null embryos died on embryonic day 10.5 as a consequence of incomplete vascular development. Foxo1-null embryonic and yolk sac vessels were not well developed at embryonic day 9.5, and Foxo1 expression was found in a variety of embryonic vessels, suggesting a crucial role of this transcription factor in vascular formation. On the other hand, both Foxo3a- and Foxo4-null mice were viable and grossly indistinguishable from their littermate controls, indicating dispensability of these two members of the Foxo transcription factor family for normal vascular development. Foxo3a-null females showed age-dependent infertility and had abnormal ovarian follicular development. In contrast, histological analyses of Foxo4-null mice did not identify any consistent abnormalities. These results demonstrate that the physiological roles of Foxo genes are functionally diverse in mammals.

ANKRA2 (ankyrin repeat, family A [RFXANK-like] 2) is a novel protein named for its ankyrin repeats and significant homology (51% aa identity) to RFXANK, a gene linked to bare lymphocyte syndrome (BLS), an MHC class II immunodeficiency (Masternak et al., 1998, Nagarajan et al., 1999). Since all the genes responsible for BLS have been identified, ANKRA2 can be anticipated to have an independent function. ANKRA2 interacts with the cytoplasmic domain of megalin, an endocytic receptor (Farquhar et al., 1995), and may play a role in megalin’s functions (Rader et al., 2000). In this report, we show that ANKRA2 is localized to human chromosome 5q12→q13 and not linked to RFXANK which maps to human chromosome 19p12.

Review Articles| August 07 2000 Assignment of Seta to distal mouse X chromosome by radiation hybrid mapping Subject Area: Genetics M.A. Hyatt; M.A. Hyatt aDepartment of Anatomy and Division of Neurosurgery, Virginia Commonwealth University, Richmond VA; and Search for other works by this author on: This Site PubMed Google Scholar V.W. Sykes; V.W. Sykes aDepartment of Anatomy and Division of Neurosurgery, Virginia Commonwealth University, Richmond VA; and Search for other works by this author on: This Site PubMed Google Scholar A.D. Boyer; A.D. Boyer bLudwig Institute for Cancer Research, La Jolla CA (USA) Search for other works by this author on: This Site PubMed Google Scholar K.C. Arden; K.C. Arden bLudwig Institute for Cancer Research, La Jolla CA (USA) Search for other works by this author on: This Site PubMed Google Scholar O. Bögler O. Bögler aDepartment of Anatomy and Division of Neurosurgery, Virginia Commonwealth University, Richmond VA; and Search for other works by this author on: This Site PubMed Google Scholar Cytogenetics and Cell Genetics (2000) 89 (3-4): 278. https://doi.org/10.1159/000015634 Article history Published Online: August 07 2000 Content Tools Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Tools Icon Tools Get Permissions Cite Icon Cite Search Site Citation M.A. Hyatt, V.W. Sykes, A.D. Boyer, K.C. Arden, O. Bögler; Assignment of Seta to distal mouse X chromosome by radiation hybrid mapping. Cytogenetics and Cell Genetics 1 July 2000; 89 (3-4): 278. https://doi.org/10.1159/000015634 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsCytogenetic and Genome Research Search Advanced Search Article PDF first page preview Close Modal This content is only available via PDF. 2000Copyright / Drug Dosage / DisclaimerCopyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. You do not currently have access to this content.

Research Articles| August 07 2000 Assignment of ankyrin repeat, family A (RFXANK-like) 2 (ANKRA2) to human chromosome 5q12→q13 by radiation hybrid mapping and somatic cell hybrid PCR Subject Area: Genetics K. Rader; K. Rader Department of Cellular and Molecular Medicine, Search for other works by this author on: This Site PubMed Google Scholar A.D. Boyer; A.D. Boyer Ludwig Institute for Cancer Research, and Search for other works by this author on: This Site PubMed Google Scholar M.G. Farquhar; M.G. Farquhar Department of Cellular and Molecular Medicine, Department of Pathology, Search for other works by this author on: This Site PubMed Google Scholar K.C Arden K.C Arden Ludwig Institute for Cancer Research, and Department of Medicine, University of California San Diego, La Jolla CA (USA) Search for other works by this author on: This Site PubMed Google Scholar Cytogenetics and Cell Genetics (2000) 89 (3-4): 164–165. https://doi.org/10.1159/000015604 Article history Published Online: August 07 2000 Content Tools Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Tools Icon Tools Get Permissions Cite Icon Cite Search Site Citation K. Rader, A.D. Boyer, M.G. Farquhar, K.C Arden; Assignment of ankyrin repeat, family A (RFXANK-like) 2 (ANKRA2) to human chromosome 5q12→q13 by radiation hybrid mapping and somatic cell hybrid PCR. Cytogenetics and Cell Genetics 1 July 2000; 89 (3-4): 164–165. https://doi.org/10.1159/000015604 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsCytogenetic and Genome Research Search Advanced Search This content is only available via PDF. 2000Copyright / Drug Dosage / DisclaimerCopyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Article PDF first page preview Close Modal You do not currently have access to this content.

The serine- and arginine-rich (SR) splicing factors play an important role in both constitutive and alternative pre-mRNA splicing, and the functions of these splicing factors are regulated by phosphorylation. We have previously characterized SRPK1 (SFRSK1) and SRPK2 (SFRSK2), which are highly specific protein kinases for the SR family of splicing factors. Here we report the chromosomal localization of the mouse and human genes for both kinases. SRPK1 probes detected two loci that were mapped to mouse Chromosomes 17 and X using The Jackson Laboratory interspecific backcross DNA panel, and SRPK2 probes identified a single locus on mouse Chromosome 5. Using a somatic cell hybrid mapping panel and by fluorescencein situhybridization, SRPK1 and SRPK2 were respectively mapped to human chromosomes 6p21.2–p21.3 (a region of conserved synteny to mouse Chromosome 17) and 7q22–q31.1 (a region of conserved synteny to mouse Chromosome 5). In addition, we also found multiple SRPK-related sequences on other human chromosomes, one of which appears to correspond to a SRPK2 pseudogene on human chromosome 8.

CTAG was initially cloned from an esophageal squamous cell carcinoma cDNA expression library by immunoscreening with autologous patient’s serum. CTAG mRNA is expressed in a proportion of human cancers in a lineage-nonspecific fashion, whereas its expression in normal tissues is restricted to testis and ovary only. This expression pattern suggests that the CTAG product (NY-ESO-1) is an aberrantly activated tumor antigen and can potentially be an antigenic target for tumor vaccination. In the present study, we isolated human genomic clones of CTAG and established its genomic organization. By somatic cell hybrid studies and fluorescence in-situ hybridization, we localized this gene to chromosome Xq28, a region that also contains members of MAGE, a gene family that encodes several immunogenic tumor antigens with the characteristic cancer/testis expression pattern.