Objective To observe the expression and clinical significance of kang ai 1 (Kai1) in the process from the progression from colorectal polyps to cancer, invasion and metastasis.Methods Immunohistochemistry was used to examine the expression of Kai1 in the colorectal tissues, colorectal adenoma tissues and colorectal carcinoma tissues.Real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) was used to detect Kai1 mRNA expression.Results The expression of Kai1 protein was positive in 18 cases of colorectal carcinoma (45.00%), significantly lower than that in colorectal adenoma (91.25%) and colorectal tissues (100.00%), respectively, with significant differences among them (P=0.031).FQ-PCR showed that the expression of Kai1 mRNA in colorectal carcinoma, colorectal adenoma and normal colorectal mucosa was incresed by 2.038±0.933, 9.983±4.337 and 12.260±2.182 respectively (F=11.960, P=0.015).The expression of Kai1 protein and mRNA had a significant correlation with the lymph node metastasis of patients with colorectal carcinoma (P=0.011).ConclusionThe abnormal expression of Kai1 protein and mRNA is related with the carcinogenesis and the development of colorectal carcinoma.

目的 采用压力-容积(P-V)环研究压力超负荷对大鼠左心室血流动力学的影响.方法 13只SD大鼠随机分为2组:假手术(Sham)组(n=7)和心力衰竭(HF)组(n=6).HF组采用腹主动脉缩窄法制备模型,Sham组只穿线不缩窄.对两组大鼠进行血流动力学检测,大鼠麻醉后经右颈总动脉向左心室内插入Millar导管(SPR-838),记录两组大鼠的P-V变化.结果 与Sham组相比,HF组大鼠的心脏(P＜0.01)和肺脏(P＜0.05)重量均显著增加.在稳定状态的P-V环中可以看到,和Sham组相比,在压力超负荷的影响下,HF组左心室P-V环明显增高、右移,P-V曲线斜率也明显高于Sham组,左心室收缩/舒张末期压力(Pes/Ped)、收缩/舒张末容积(Ves/Ved)、压力衰退的时间常数(Tau)均增高(P＜0.05),而室内压最大上升速率(dP/dtmax)、左室压力最大下降速率(-dP/dtmin)、射血分数(EF)和每搏输出量(SV)均出现降低(P＜0.05).结论 压力负荷型慢性HF大鼠左心室发生了舒张和收缩功能障碍,并且伴有一定程度的左心室肥大.P-V环能够准确地定性、定量评价大鼠的心功能.

目的 探讨急性胰腺炎( AP)患者外周血淋巴细胞亚群的变化情况. 方法 应用流式细胞仪四色荧光分析37例轻症急性胰腺炎( MAP)患者和22例重症急性胰腺炎( SAP)患者的T淋巴细胞亚群、B淋巴细胞及NK细胞的比例变化,并与30例健康对照组进行对比分析. 结果 SAP患者 CD3 +T细胞、CD4 +T细胞、CD16 +CD56 +NK细胞百分比及CD4 + /CD8 +比值与对照组相比明显降低(P<0. 05),CD8 +T细胞百分比较对照组明显升高(P<0. 05);MAP患者CD4 +T细胞百分比较对照组明显降低(P<0. 05). 结论 AP患者尤其是SAP患者存在免疫功能损害,这可能是SAP易发生严重感染的重要原因.

Objective To analyze the diversity variation and the fingerprints difference of intestinal microflora between diarrhea patients with the fecal leukocytes and health people. Methods 30 cases of normal people and 30 cases of di-arrhea patients in No.252 Hospital of PLA were selected, whose total fecal DNA were used as model, were divided into control group and diarrhea group. Intestinal Bifidobacterium, Lactobacillus, Escherichia Coli, Enterococci bacteria number were detected by quantitative fluorescence method, the difference of intestinal flora diversity in the two groups was detected by ERIC-PCR fingerprint method. Results The quantities of intestinal microflora in the control group and di-arrhea group:Bifidobacterium spp.[(8.85±0.57) copies/g vs(8.28±0.68) copies/g];Lactobacillus spp.[(8.36±0.45) copies/g vs(7.79±0.39) copies/g], there were significant differences (P<0.05). There was no difference about the Escherichia Coli and Enterococci in the two groups (P>0.05). The bands of ERIC-PCR fingerprints in diarrhea group were obviously declined. Conclusion The constitution of the intestinal microflora is changed in diarrhea patients and the quantity of intestinal flora is significant declined.

在全世界范围内，心血管疾病造成了30%的病死率。冠状动脉粥样硬化性心脏病是心肌梗死和心力衰竭的首要病因。动脉粥样硬化及其并发症不仅仅是简单的脂类失衡，而是环境和遗传的影响导致生物系统和免疫系统的瓦解[1]。在心肌梗死和心力衰竭中几乎牵涉到每种免疫细胞，一些免疫细胞可导致动脉粥样硬化、心肌梗死和心力衰竭，而另一些则可以防止相应的病变。因此免疫细胞在心肌梗死和心力衰竭中的作用研究日益成为热点。

Objective To observe cardiomyocyte necrosis in the rats induced by acute exhausted exercise and determine the protective effects of Xinfukang oral liquid (a traditional Chinese medicine) on the necrosis. Methods A total of 40 male Sprague-Dawley (SD) rats were randomly divided into 4 groups:control group, the low dose Xinfukang oral liquid [5ml/(kg·d)]+acute exhaustive group, the high dose Xinfukang oral liquid [10ml/(kg·d)]+acute exhaustive group, and acute exhaustive group (n=10 for each group). The Xinfukang oral liquid groups were treated with Xinfukang oral liquid at corresponding doses for 6 weeks by intragastrical administration before model induction, and the rats from the control group and acute exhaustive group were given normal saline instead of Xinfukang oral liquid. Acute exhausted exercise was carried out according to the classical Thomas’ induction, and then identified by the rats’ behaviors and signs. Myocardial necrosis was observed by transmission electron microscopy. DNA ladder were detected to observe myocardial cell DNA degradation. Western blotting was used to analyze the expression of total extracellular signal-regulated kinase (ERK) and phosphorylated ERK (p-ERK) in all groups. Results Transmission electron microscopy indicated that exhausting exercise group showed significantly more serious mitochondria swelling, obvious myocardial necrosis and DNA degradation. Compared with exhausted group, pretreatment of Xinfukang oral liquid resulted in attenuated injury in both myocardial cells and DNA integrity, and a increased ratio of p-ERK expression(P<0.05). Conclusion Pretreatment of Xinfukang oral liquid exerts a preventive effect on cardiomyocyte necrosis induced by acute exhaustive exercise.

Objective To investigate the identification effect of 16S rRNA gene library method in bacterium flora analysis of infected samples.Methods Fresh pus samples or exudate of 70 trauma patients were collected.The 16S rRNA gene cloning library was established with 16S rRNA gene fragment in amplifiable samples of bacterial universal primers.Positive clones were sequenced,and amount and category of infected bacterials were analyzed.Results The total of 150 clones were selected nand 7 restriction fragment length polymorphism(RFLP) types and 7 kind of microbe which were used for sequencing.Enterococcus faecium was the most one(88% of total clones),Enterococcus(2% of total clones),5 of these sequences were closely related to the uncultured bacteria fungi of 16S rRNA gene sequence similarity(2% of total clones respectively).Conclusion The results indicate that the rich Enterococcus faecium,and 16S rRNA gene library method may be an effective method for isolating uncultured bacteria in trauma infected samples.

Numerous microbes inhabit the human surface compound a microbiot,many of which are uncharacterized or uncultivable.Metagenomics,or the culture-independent genomic analysis are essential to understand the genetic diversity,population structure,and ecological roles of the majority of microbiot.Metagenomics has been previously employed for pathogen discovery,resistant gene,metabolic disease and establish microbial pattern of health people.