This paper explores women smallholder livestock farmers’ barriers to effective participation in the livestock vaccine value chain (LVVC) and strategies for optimising the use of vaccines against poultry (Newcastle disease), and goat diseases (Peste des Petits Ruminants) in Sembabule District, Uganda. Using the three domains of empowerment theory: intrinsic, instrumental, and collective agency combined with USAID’s gender analysis framework, we found that limited control over resources, and inadequate gender responsive policies hinder women’s participation. Self-help groups increased opportunity for livestock ownership, autonomy, and decision-making. Engagement of women in the LVVC, is an entry point for improved vaccine accessibility, and agency.

There is increasing demand for poultry products globally; however, the feed costs are so high covering over 80% of total costs of production.Present research investigated the growth performance and carcass characteristics of commercial broilers feeding on inclusion of Mexican sunflower leaf feed (MSLF) as an inexpensive alternative feed.Two experienced farmers were identified and selected and each allocated 420 chicks that were fed on varying levels of MSLF up to the point of sale.Data on growth performance and carcass characteristics were collected and analyzed.Results showed that average final body weight (AFBW), average weight gain (AWG), and average feed conversion ratio (FCR) were significantly different.Significant reduction in the AFBW of broilers was as well observed as the inclusion rate of MSLF increased from 0 to 12% with MSLF12 recording the lowest (436.70g).MSLF0 recorded the highest weight gain (1208.63g)whereas MSLF12, the lowest (394.17g).Results further showed a steady reduction in feed consumption (FC) across various rates of MSLF.MSLF0 expressed the highest (120.39g).FCR was highest for MSLF12 (0.24).A significant difference (P >0.05) among the various inclusion rates was noted for slaughter and carcass weights.MSLF8 was significantly lower and different when compared to MSLF0, MSLF1, MSLF2 MSLF3, and MSLF4 for slaughter weight.The slaughter and carcass weights of birds fed 0%, 1%, 2%, and 3% MSLF diet were higher than those fed on 4%, 8%, and 12% MSLF.In conclusion, growth performance and carcass characteristics and cut portion yield are significantly reduced with increasing inclusion rate of MSLF.Inclusion rates of MSLF3, MSLF2, and MSLF1 are the most optimum and can be used without affecting performance of broilers.

This paper explores the role of extension training in enhancing women smallholder livestock farmers’ participation in and benefiting from the livestock vaccine value chain (LVVC). The emphasis was on the Peste des Petits Ruminants (PPR) vaccine for goats and New Castle Disease vaccine (ND) for chicken. Using the Focus Group discussions, Participatory Impact Assessment, Outcome mapping, Key Informant Interviews, Questionnaire Survey and Kirkpatrick model of change, the study assessed the impact of extension training which equipped extensionists and women farmers with knowledge, skills, attitude and practice in livestock vaccination and empowerment. The study found that extension training made a tremendous impact on the availability of knowledge and information to women farmers as well as in empowering women’s self-autonomy, improved economic status, decision making role in both farm related and family related matters, reduced Gender Based Violence cases and improved productivity in the community.

Abstract Background: Animal diseases that are endemic like tsetse transmitted trypanosomosis cause the continuous expenditure of financial resources of livestock farmers and loss of productivity of livestock. Estimating the cost of controlling animal trypanosomosis can provide evidence for priority setting and targeting cost-effective control strategies. Methodology: A cross-sectional survey to estimate the economic cost of bovine trypanosomosis was conducted in cattle-keeping communities living around Murchision falls National Park, in Buliisa district Uganda. Data was collected on herd structure, the cost of treatment and control, prevalence of morbidity and mortality rates due to trypanosomosis, and salvage sales losses in cattle herds in the last year. Results: In this study, 55.4% (n = 87) of the households reported their cattle had been affected by trypanosomosis during the previous last year. There was a high economic cost of trypanosomosis (USD 653) per household in cattle-keeping communities in Buliisa district of which 83% and 9% were due to mortality and milk loss respectively/ High mortality loss was due to low investment in treatment. The study showed that prophylactic treatment 3 times a year of the whole herd of cattle using Samorin ® (Isometamidium chloride) at a cost of USD 110 could drastically reduce cattle mortality loss due to trypanosomosis due to trypanosomosis with a return on investment of USD 540 annually per herd. This could be coupled with strategic restricted insecticide spraying of cattle with deltamethrin products. Conclusion: The results show a high economic cost of trypanosomosis in cattle-keeping communities in Buliisa district, with cattle mortality contributing the largest proportion of the economic cost. The high mortality loss was due to low investment in treatment of sick cattle.

Background: Urinary Tract Infection (UTI) is a disease of the urinary system. This type of infection involves urethritis, pyro nephritis, or cystitis. Symptoms can be pelvic pain, increased urge to urinate, pain on passing out urine, and blood in the urine (blander infections). A kidney UTI may cause back pain, nausea vomiting, and fever. Methods: This study was a cross-sectional-quantitative study carried out among 300 pregnant mothers attending ANC at Kawempe regional referral hospital in August 2021. Questionnaires were administered to get the biodata of Participants. Urine samples were collected using sterile containers. Urine culture was performed, urine chemistry using ten parameter strip then urine microscopy was done. Chemistry was done using ten parameter strips, urine microscopy and then later culture and sensitivity was done on urine samples with proteinuria. Results: Urine samples with proteinuria were 5.7 % (n=17), more than half of the Urine Samples 52%( n=156) had positive White blood cell cells and 48 %( n=144) had no abnormality detected. Culture on urine samples showed the following isolation, Escherichia.coli 24.3 %( n=9), Klebsiella pneumonia 21.6 %( n=8), Pseudomonas euroginosa 18.9 %( n=7) Staphylococcus aureus 16.22 %(n=6) Enterococcus spp 2.7%(n=1) and Candida albicans16.2 %( n=6). Conclusion: UTI is an infection of some part of your body’s urinary system which may include: kidneys, ureters, bladder, and urethra. Organisms causing UTIs in pregnancy are the same uropathogens that commonly cause UTI in non-pregnant patients with Escherichia coli being the most commonly isolated organism. Other bacteria include klebishella pneumoniae, staphulococuus, streptococcus, enterococcus, and pseudomonas. Recommendations: Kawempe National Referral Hospital. Should improve the Laboratory to perform microbiology tests.

Poultry meat is one of the fastest growing components of Uganda’s meat production, consumption, and trade. However, it is estimated that more than 80 % of the small holder dominated sub-sector use traditional family-based production systems which limit production efficiency. This research analyzed the farmers’ characteristics and productivity of commercial broiler production units in Mukono District. Using a cross-sectional survey, 302 broiler farmers were randomly selected from five sub-counties of Mukono District. Data were analyzed using the descriptive statistics to characterize broiler farmers, whereas, ordinary least square regression analysis was carried out to assess factors affecting productivity of commercial broiler production units. Results showed that majority were males (53.31%) and married (92.72%). Most (60.26%) had primary as their highest level of education attained. The average age was 45 years with average household size of 7 members. Similarly; majority (91.04%) had land under poultry farming ranging from 0.25 to 3 acres. Feeds cost (P≤0.05), veterinary cost (P≤0.001), farmer’s level of education (P≤0.05), extension services (P≤0.001), age of a household head (P≤0.001), land ownership (P≤0.05), and labour type (P≤0.05), showed a positive and significant influence on productivity, whereas, poultry farming experience (P≤0.001), land under poultry farming (P≤0.001), distance to the nearest market (P≤0.05), and record keeping (P≤0.05), negatively and significantly influenced productivity. In conclusion, education level, land under poultry farming, and membership to a group were distinct among farmers who were productive and those that were not. Productivity of commercial broiler farming was majorly influenced by several farmers’ socio-economic characteristics.

This paper describes the institutional context that shapes the visibility and positioning of women along the Peste des Petits Ruminants (PPR) and Newcastle Disease (ND) vaccine value chains for Sembabule district of Uganda. It examines the institutional barriers and opportunities that affect women’s empowerment derived from inclusion of women in the decision-making processes along the livestock vaccine value chain (LVVC) and that can support viable women-centered and owned enterprises, at the vaccine development, delivery, distribution and use level. Qualitative data analysis tools such as focus group discussions, focus meals, jar voices and key informant interviews were used. Using outcome mapping, a stakeholder analysis of the critical partners in the PPR and ND value chain was done involving the regulators, vaccine manufacturers, importers, distributors, agrovets, public and private veterinary service deliverers, local leaders, women groups, and farmers. The study concluded that training related to gender equality and livestock vaccines, infrastructural and technical support to the poultry and goat women and men farmers and other chain actors are inadequate in themselves to increase vaccine adoption and improve livestock productivity in Sembabule district. Strategies that promote gender-transformative collaborative efforts among the LVVC actors and build viable gender-transformed women groups and networks are critical to increase women’s participation in and benefit from the livestock vaccine value chain.

Abstract Background - Animal diseases that are endemic like tsetse transmitted trypanosomosis cause continuous expenditure of financial resources of livestock farmers and loss of productivity of livestock. Estimating the cost of controlling animal trypanosomosis can provide evidence for priority setting and targeting cost effective control strategies.Methodology A cross sectional survey to estimate the economic cost of bovine trypanosomosiswas conducted in cattle keeping communities living around Murchision falls National Park, in Buliisa district Uganda. Data was collected on herd structure, the cost of treatment and control, prevalence of morbidity and mortality rates due to trypanosomosis, salvage sales losses in cattle herd in the last one year.Results- In this study, 55.4% (n= 87) of the households reported their cattle had been affected by trypanosomosis during the previous last year. There was a high economic cost trypanosomosis (USD 653) per household in cattle keeping communities in Buliisa district of which 83% and 9% was due to mortality and milk loss respectively/ High mortality loss was due to low investment in treatment. The study showed that prophylactic treatment 3 times a year of the whole herd of cattle using Samorin ® (Isometamidium chloride) at a cost of USD 110 could drastically reduce cattle mortality loss due to trypanosomosis due to trypanosomosis with return on investment of USD 540 annually per herd. This could be coupled with strategic restricted insecticide spraying of cattle with deltamethrin products.Conclusions. The results show a high economic cost of trypanosomosis in cattle keeping communities in Buliisa district with mortality of cattle contributing the largest proportion of the economic cost. The high mortality loss was due to low investment in treatment of sick cattle

Abstract Background Bovine trypanosomosis transmitted by tsetse flies is a major constraint to cattle health and productivity in all sub-Saharan countries, including Uganda. The objectives of this study were to determine the prevalence of bovine trypanosomosis and identify its associated risk factors and the species of trypanosomes associated with the disease. Methodology A cross-sectional study was conducted around Murchison Falls National Park, Uganda from January 2020 to April 2020. Trypanosomes were detected in blood samples by PCR analysis targeting the internal transcribed spacer 1 (ITS-PCR assays), and trypanosomes in positive blood samples were sequenced. Results Of 460 blood samples collected and tested, 136 (29.6%) were positive for trypanosome infections and 324 (70.4%) were negative. The overall trypanosome prevalence was 29.6% (95% confidence interval 25.4–33.8%), attributed to three trypanosome species. Of these three species, Trypanosoma vivax was the most prevalent (n = 130, 28.3%) while the others were detected as mixed infections: T. vivax + Trypanosoma congolense (n = 2, 0.4%) and T. vivax + Trypanosoma evansi (n = 1, 0.2%). There were significant differences in trypanosome prevalence according to sex (χ 2 = 62, df = 1, P < 0.05), age (χ 2 = 6.28, df = 2, P = 0.0043) and cattle breed (χ 2 = 10.61, df = 1, P = 0.001). Conclusions Trypanosomosis remains a major limitation to cattle production around Murchison Falls National Park and interventions are urgently needed. In our study, the prevalence of trypanosome infections was high, with T. vivax identified as the most prevalent species. Age, sex and breed of cattle were risk factors for trypanosome infection. Graphical Abstract

A mixed method survey was conducted among pastoral and agro pastoral communities surrounding Murchison Falls National Park, Uganda to assess knowledge, attitudes and practices about control of bovine trypanosomosis. A total of 96.8% (n = 152) of the participants had seen tsetse flies, and close to 91.7% (n = 116) of the participants had heard about bovine trypanosomosis. Bovine trypanosomosis was reported as a major disease in their area by about 73.9% (n = 116). There was a significant difference (P < 0.05) in the level of awareness and perception about tsetse and bovine trypanosomosis across the study sub counties. The majority of the farmers (60.5%) stated that grazing near national parks was the main cause of bovine trypanosomosis. A small proportion of farmers associated sharing grazing land and watering points with wildlife (19.1%) and grazing cattle in tsetse fly-infested areas (8.3%) as the causes of trypanosomosis. The communities in the study sub counties were aware of at least one or two clinical signs of bovine trypanosomosis. Spraying cattle with insecticide and avoiding grazing animals in tsetse-infested areas were the control practices. Curative trypanocides were mainly used to treat their cattle against trypanosomosis. Bush clearing, targets and traps as tsetse fly control measures were less practiced by the farmers. Treatment of cattle was based on observation of clinical signs due to absence of blood diagnostic facilities. Implementing regular tsetse fly population monitoring surveys and promotion of disease rapid diagnostic tools at farm level as long-term strategies are key for effective control of the disease.

Background: Bovine trypanosomosis transmitted by tsetse flies is a major constraint to cattle health and productivity in sub- Saharan countries including Uganda. The objectives of this study were to determine the prevalence of bovine trypanosomosis and its associated risk factors and the species of trypanosomes associated with the disease. Methodology: A cross sectional study was conducted around Murchision Falls National Park, Uganda from January 2020 to April 2020. Blood samples were collected from 460 cattle and were screened for trypanosomes using ITS-PCR. Results: From 460 samples collected, 136 (29.6%) of the samples were positive for trypanosomosis infections while 324 (70.4%) samples were negative. The overall trypanosome prevalence was 29.6% (95% CI 25.4-33.8). T. vivax (n = 130, 28.3%) was the most prevalent trypanosome specie detected and two mixed infection types (T.vivax + T. congolense (n = 2, 0.4%) and T.vivax + T. brucei (n = 1.0, 0.2% ) were detected during the analysis. There was a significant difference when Trypanosome prevalence was compared with sex, age, breed of the sampled animals (P < 0.05 for sex), (P = 0.0043 for age) and (P = 0.001 for breed). Conclusion ; Trypanosomosis is still a major limitation to cattle production around Murchision Falls National Park and interventions are urgently needed .The prevalence of trypanosomosis was high and T.vivax was identified as the major trypanosome species in the cattle herd.

Most capacity building efforts to investigate and counter emerging infectious diseases have focused on supporting public health agencies. However, to improve the understanding of the epidemiology, and outcome of diseases, aid in their detection and treatment and increase public participation in prevention and control, gender roles must be considered. Gender plays a significant role in shaping infectious disease response. In the most recent Ebola Outbreak in the West African region, glaring gender disparities were apparent as Ebola spread through nations decimating families. Policy implementers, practitioners and researchers were slow to recognize the gender implications, ask why, and build responses accordingly. A report that examined the avian influenza crisis in South East Asia in 2008 concluded that women were clearly in the frontline defense against the disease both as caretakers of the poultry and the families, and yet strategies to combat avian influenza did not consider their roles and potential contribution to the prevention and response. Makerere University, Uganda with the support of Tufts University developed a Gender, One Health and Infectious Disease short course that allows public health specialists to address gender gaps, and explore how gender, the realm of emerging pandemic threats and One Health intersect and how policies can be developed and/or implemented to address those gaps. The week long short course targets in service personnel in multiple disciplines, the private sector, faculty and students from OHCEA institutions and Africa. The course themes apply gender analysis tools to disease surveillance, response, and control and address gender sensitive emergency response planning.

A study was undertaken along the Kenya–Uganda border in four districts of Tororo and Busia (Uganda) and Busia and Teso (Kenya) to understand smallholder farmers’ knowledge, practices and awareness of biosecurity measures. Information was collected by administering questionnaires to 645 randomly selected pig households in the study area. In addition, focus group discussions were carried out in 12 villages involving 248 people using a standardized list of questions. The outcome suggested that there was a very low level of awareness of biosecurity practices amongst smallholder farmers. We conclude that adoption of specific biosecurity practices by smallholder farmers is feasible but requires institutional support. There is a clear requirement for government authorities to sensitize farmers using approaches that allow active participation of farmers in the design, planning and implementation of biosecurity practices to enable enhanced adoption.

Purpose - Agricultural education and training (AET) institutions will play a strategic role in helping to prepare Africa's rapidly growing youth populations for productive careers in agriculture and related agri-businesses. The purpose of this paper is to examine the magnitude of skills and youth employment needs emanating from high-population growth rates. It then explores how agricultural education institutions are responding to these challenges in four different countries at different levels of food system development: South Africa tier 1, Tanzania in tier 2 and Malawi and Uganda in tier 3.Design/methodology/approach - Demographic and school enrollment data provide information on themagnitude of jobmarket entrants at different levels of education while Living Standards Measurement Studies in the respective countries provide a snapshot of current skill requirements in different segments of the agri-food system. In order to evaluate AET responses, the authors have conducted country-level reviews of AET systems as well as in-depth assessments at key tertiary AET institutions in each of the four case study countries.Findings - Growth rates in primary school enrollments are high in sub-Saharan Africa. At the same time, because of budgetary constraints, transition rates decline rapidly - about 40 percent from primary to secondary and 7 percent from secondary to tertiary. As a result, substantial numbers of primary and secondary school graduates seek jobs.Research limitations/implications - The case study countries are limited to four. Had more financial resources and time been available, researchers could have spread further afield and in so doing increasing the precision of the results.Originality/value - Estimation of the number of primary and secondary school leavers seeking employment because of failure to proceed to the next level of education. Estimation of the level of education shares in the various components of the agri-food system.

A cross-sectional survey was carried out to assess risk factors associated with occurrence of African swine fever (ASF) outbreaks in smallholder pig farms in four districts along Kenya-Uganda border. Information was collected by administering questionnaires to 642 randomly selected pig households in the study area. The study showed that the major risk factors that influenced ASF occurrence were purchase of pigs in the previous year ( p < 0.000) and feeding of pigs with swill ( p < 0.024). By employing cluster analysis, three clusters of pig production types were identified based on production characteristics that were found to differ significantly between districts. The most vulnerable cluster to ASF was households with the highest reported number of ASF outbreaks and composed of those that practiced free range at least some of the time. The majority of the households in this cluster were from Busia district in Uganda. On the other hand, the least vulnerable cluster to ASF composed of households that had the least number of pig purchases, minimal swill feeding, and less treatment for internal and external parasites. The largest proportion of households in this cluster was from Busia district Kenya. The study recommended the need to sensitize farmers to adopt proper biosecurity practices such as total confinement of pigs, treatment of swill, isolation of newly purchased pigs for at least 2 weeks, and provision of incentives for farmers to report suspected outbreaks to authorities and rapid confirmation of outbreaks.

Background Brucellosis is a zoonotic disease of veterinary, public health, and economic significance in most developing countries, yet there are few studies that show integrated human and veterinary health care intervention focusing on integration at both activity and actors levels. The aim of our study, therefore, was to explore community perceptions on integration of animal vaccination and health education by veterinary and public health workers in the management of brucellosis in Uganda. Methods This study used a qualitative design where six Focus Group Discussions (FGDs) that were homogenous in nature were conducted, two from each sub-county, one with the local leaders, and another with pastoralists and farmers. Five Key Informant Interviews (KIIs) with two public health workers and three veterinary extension workers from three sub-counties in Kiruhura district, Uganda were conducted. All FGDs were conducted in the local language and tape recorded with consent from the participants. KIIs were in English and later transcribed and analyzed using latent content data analysis method. Results All the groups mentioned that they lacked awareness on brucellosis commonly known as Brucella and its vaccination in animals. Respondents perceived improvement in human resources in terms of training and recruiting more health personnel, facilitation of the necessary activities such as sensitization of the communities about brucellosis, and provision of vaccines and diagnostic tests as very important in the integration process in the communities. The FGD participants also believed that community participation was crucial for sustainability and ownership of the integration process. Conclusions The respondents reported limited knowledge of brucellosis and its vaccination in animals. The community members believed that mass animal vaccination in combination with health education about the disease is important and possible if it involves government and all other stakeholders such as wildlife authorities, community members, local to national political leaders, as well as the technical personnel from veterinary, medical and public health sectors since it affects both humans and animals.

Background: Human brucellosis is prevalent in both rural and urban Uganda, yet most cases of the disease in humans go unnoticed and untreated because of inaccurate diagnosis, which is often due to the disease not manifesting in any symptoms. This study was undertaken to describe trends in laboratory-confirmed human brucellosis cases at three health facilities in pastoralist communities in South-western, Uganda.Methods: Data were collected retrospectively to describe trends of brucellosis over a 10-year period (2003-2012), and supplemented with a prospective study, which was conducted from January to December 2013. Two public health facilities and a private clinic that have diagnostic laboratories were selected for these studies. Annual prevalence was calculated and linearly plotted to observe trends of the disease at the health facilities. A modified Poisson regression model was used to estimate the risk ratio (RR) and 95 % confidence intervals (CIs) to determine the association between brucellosis and independent variables using the robust error variance.Results: A total of 9,177 persons with suspected brucellosis were identified in the retrospective study, of which 1,318 (14.4 %) were confirmed cases. Brucellosis cases peaked during the months of April and June, as observed in nearly all of the years of the study, while the most noticeable annual increase (11-23 %) was observed from 2010 to 2012. In the prospective study, there were 610 suspected patients at two public health facilities. Of these, 194 (31.8 %) were positive for brucellosis. Respondents aged 45-60 years (RR = 0.50; CI: 0.29-0.84) and those that tested positive for typhoid (RR = 0.68; CI: 0.52-0.89) were less likely to have brucellosis.Conclusions: With the noticeable increase in prevalence from 2010 to 2012, diagnosis of both brucellosis and typhoid is important for early detection, and for raising public awareness on methods for preventing brucellosis in this setting.

Dairy cattle breeding is an important technology in the enhancement and promotion of dairy production in Uganda. The introduction of germplasm through AI is crucial to enhance the production potential of the local breeds. A study was conducted in six districts of Uganda in the central region using a questionnaire survey involving 450 randomly selected households to profile the dairy breeding services in use and investigate the factors that affect the success of dairy breeding focusing on AI. Adoption of the AI service was highly (P<0.05) dependent on ava ilability of extension services, record keeping practice (P<0.05), and availability of milk markets (P<0.05). On the other hand AI adoption was independent of formal education, age of farmer, labor availability, and feed/water availability (P>0.05). Use or nonuse of AI did not significantly (P>0.05) influence the sex of the calf born. While preference for AI was marked, very few farmers actually used it. This implies that focus should be put on improved AI service delivery alongside improved extension services.

BACKGROUND:Brucellosis is one of the most common zoonotic infections globally. Lack of knowledge about brucellosis may affect the health-seeking behavior of patients, thus leading to sustained transmission in these communities. Our study assessed knowledge and perceptions of brucellosis among pastoral communities adjacent to Lake Mburo National Park (LMNP), Kiruhura District, Uganda.METHODS:A community cross-sectional questionnaire survey involving 371 randomly selected household heads from three sub-counties neighboring LMNP were interviewed between June and August 2012. Data collected included communities' knowledge on causes, symptoms, transmission, treatment, prevention and risk factors of brucellosis. Multivariable logistic regression analysis was performed to explore strength of association between overall knowledge of brucellosis and various individual factors using odds ratios and 95% confidence intervals.RESULTS:Only 70 (19%) knew the symptoms of brucellosis in animals, and three quarters (279, 75.5%) mentioned joint and muscle pain as a common symptom in humans. Almost all participants (370, 99.3%) had ever heard about brucellosis, majority (311, 84.7%) believed it affects all sexes and two thirds (67.7%) of the respondents believed close proximity to wildlife contributes to the presence of the disease. Almost all (352, 95.4%) knew that brucellosis in humans could be treatable using modern drugs. The main routes of infection in humans such as consumption of unpasteurized dairy products were known by 97% (360/371); eating of half-cooked meat by 91.4% and eating contaminated pasture in animals by 97.4%. There was moderate overall knowledge of brucellosis 197 (53.1%). Factors associated with higher overall knowledge were being agro-pastoralists (aOR: 2.08, CI: 1.17-3.71) compared to pure pastoralists while those who reported that the disease was a health problem (aOR: 0.18, CI: 0.06-0.56) compared to those who said it was not were less likely to be knowledgeable.CONCLUSIONS:There was moderate overall knowledge of human and animal brucellosis among the participants. Majority of the participants believed that close proximity to wildlife contributes to the presence of the disease in the area. There is a need for collaboration between the public health, veterinary and wildlife sectors to provide health education on brucellosis for better management of the disease in the communities.