OBJECTIVE:Research purpose was to study phagocytic activity and a oxygen depended metabolism of blood neutrophils (NF) at patients with a renal cell carcinoma in dynamics - before operation and after surgical treatment. METHODs; Patients with a locally invasive renal cell carcinoma (T3N0M0) were put under observation before surgical treatment (n =84) and in 10 days after a radical nephrectomy (n =54) at the age of 45-55 years. The 56 conditionally healthy donors combine into the control group. Indicators of functional activity, the spontaneous and induced lyuminol- and lyutsigenin-dependent chemiluminescence (CHL) NF of pedipheral blood was studied.RESULTS:During the period before operation time of an exit to a maximum of spontaneous and stimulated lyuminol-dependent HL is slowed down (p <0.004 and p <0.006 respectively); indicators of the area of lyutsigenin-dependent HL NF of blood concerning indicators of control group are increased (p <0.01 for spontaneous, p <0.004 for the reaction induced by lyutsigenin). In 10 days after surgical treatment the quantity of active ingestion rate decreased (p <0.017 in the test with S. epidermidis, p <0.036 for test with latex); time of an exit to a maximum of spontaneous and stimulated lyuminol-dependent remained increased (p <0.006 and p <0.008 respectively) in comparison with indicators of healthy donors. The amount of an index of activation of lyuminol-dependent as in comparison with control (p <0.037), and relative to the period before operation increased (p <0.048). The area of spontaneous lyutsigenin-dependent HL NF of blood relative to indicators of control group was still increased (p <0.011), decrease in this parameter in comparison with amount of group before operation was however registered (p <0.004).CONCLUSION:At patients with renal cell carcinoma in 10 days after surgical treatment of NF of blood were characterized by the strengthened production of high-energy oxidizers and the reduced phagocytic activity that testifies to a functional and metabolic imbalance.
ИЗМЕНЕНИЕ КОЛИЧЕСТВА И ФУНКЦИОНАЛЬНО-МЕТАБОЛИЧЕСКОЙ АКТИВНОСТИ НЕЙТРОФИЛОВ ПЕРИФЕРИЧЕСКОЙ КРОВИ У БОЛЬНЫХ РАКОМ МОЧЕВОГО ПУЗЫРЯЛ
The aim of the research. To study the quantitative indicators, parameters of phagocytic activity and chemiluminescence response of peripheral blood neutrophils in patients with muscle-invasive bladder cancer before surgical treatment. Materials and methods. The study involved 44 patients with muscle-invasive bladder cancer at the age of 45-55 years old. Control group was consisted of 39 healthy donors. To determine the phagocytic index and phagocytic number were carried out the tests with latex particles and S. epidermidis culture. The assessment of luminol dependent chemiluminescence (ChL) of neutrophils was carried out by the method De Sole et al. (1983). Results. In patients with muscle-invasive bladder cancer was detected the increase of percentage and absolute number of neutrophils in the peripheral blood, were found phagocytic activity changes and the chemiluminescent response. Conclusion. The observed changes reflect the functional-metabolic stress of neutrophilic granulocytes in patients with muscle-invasive bladder cancer.
Abstract. We have examined ninety-three patients with locally advanced renal cell cancer at the age of 45 to 55 years. Immunological testing was performed before radical nephrectomy and 14 days after surgery. The cellular and humoral immune parameters and cell chemiluminescence were studied both at baseline and in stimulated cells. We found changes of immunophenotypic profile of blood lymphocytes, serum immunoglobulin indexes and chemiluminescence response of blood neutrophils, dependent on tumor histology.
Abstract. Twenty patients with basal cell skin carcinoma at the age of 56 to 70 years have been included into study. We have investigated multiple parameters of cellular and humoral immunity, features of phagocytic activity, spontaneous and stimulated chemiluminescence of peripheral blood neutrophils. Distinct changes in blood lymphocyte subsets have been assessed by immunophenotyping. Changes in serum immunoglobulin profiles and increased levels of circulating immune complexes were revealed. Altered phagocytic activity and oxygen-dependent metabolism of blood neutrophils were also detected.
Objective: Research purpose was to study phagocytic activity and a oxygen depended metabolism of blood neutrophils (NF) at patients with a renal cell carcinoma in dynamics — before operation and after surgical treatment. Methods: Patients with a locally invasive renal cell carcinoma (T 3 N 0 M 0 ) were put under observation before surgical treatment (n =84) and in 10 days after a radical nephrectomy (n =54) at the age of 45–55 years. The 56 conditionally healthy donors combine into the control group. Indicators of functional activity, the spontaneous and induced lyuminoland lyutsigenindependent chemiluminescence (CHL) NF of peripheral blood was studied. Results: During the period before operation time of an exit to a maximum of spontaneous and stimulated lyuminol-dependent HL is slowed down (p <0.004 and p <0.006 respectively); indicators of the area of lyutsigenin-dependent HL NF of blood concerning indicators of control group are increased (p <0.001 for spontaneous, p <0.004 for the reaction induced by lyutsigenin). In 10 days after surgical treatment the quantity of active ingestion rate decreased (p <0.017 in the test with S. epidermidis, p <0.036 for test with latex); time of an exit to a maximum of spontaneous and stimulated lyuminol-dependent remained increased (p <0.006 and p <0.008 respectively) in comparison with indicators of healthy donors. The amount of an index of activation of lyuminol-dependent as in comparison with control (p <0.037), and relative to the period before operation increased (p <0.048). The area of spontaneous lyutsigenindependent HL NF of blood relative to indicators of control group was still increased (p <0.011), decrease in this parameter in comparison with amount of group before operation was however registered (p <0.004). Conclusion: At patients with renal cell carcinoma in 10 days after surgical treatment of NF of blood were characterized by the strengthened production of high-energy oxidizers and the reduced phagocytic activity that testifies to a functional and metabolic imbalance.
Abstract. We have examined ninety-three patients with locally advanced renal cell cancer at the age of 45 to 55 years. Immunological testing was performed before radical nephrectomy and 14 days after surgery. The cellular and humoral immune parameters and cell chemiluminescence were studied both at baseline and in stimulated cells. We found changes of immunophenotypic profile of blood lymphocytes, serum immunoglobulin indexes and chemiluminescence response of blood neutrophils, dependent on tumor histology.
Twenty patients with basal cell skin carcinoma at the age of 56 to 70 years have been included into study. We have investigated multiple parameters of cellular and humoral immunity, features of phagocytic activity, spontaneous and stimulated chemiluminescence of peripheral blood neutrophils. Distinct changes in blood lymphocyte subsets have been assessed by immunophenotyping. Changes in serum immunoglobulin profiles and increased levels of circulating immune complexes were revealed. Altered phagocytic activity and oxygen-dependent metabolism of blood neutrophils were also detected
High basal production of primary active oxygen forms was detected in the peripheral blood neutrophils of patients with renal cell cancer. In vitro stimulation of neutrophils led to more rapid release of superoxide radicals into extracellular space and to a reduction of cell capacity to more intense production of primary active oxygen forms.
Individual sensitivity of blood neutrophils to IFN-α2a was studied in vitro in patients with renal cell cancer before and after the first course of reaferon therapy. Cell sensitivity changed after reaferon therapy in 85% patients. This necessitates evaluation of individual cell sensitivity not only before therapy, but also before the next course of therapy.
The dynamic supervision of 132 patients witrenal cell carcinoma (RCC) before and in 14 days after radical nephrectomy and 1,5 month after surgical treatment was determined. The findings of chemiluminescence analysis of leukocytes reactivity to interferon in vitro has shown, that optimal time for interferonotherapy at the patients with RCC in 1,5 month after radical nephrectomy, then range of cell sensitivity to interferon is extends.
Patients with renal cell carcinoma (RCC) were examined by the chemiluminescent analysis before and after surgery. The cells of subjects with the discussed pathology were found to be in the state of functionally metabolic misbalance, i.e. an increased initial level of the synthesis of active oxygen with a relatively reduced cell reactivity when additionally stimulated. Such specificity of neutrophil granulocytes of peripheral blood is more pronounced in RCC patients after surgery. Supposedly, the above phenomenon is preconditioned by a lack of metabolic reserves that could provide an adequate functional response to extra stimulation and it develops due to a prolonged tumor progression and stress brought about by surgical intervention.
