于滴水湖湖心采集底泥样品,对底泥中可培养优势菌种进行分离、纯化,并利用Biolog微生物自动分析系统进行鉴定.结果显示,滴水湖沉积物中菌落总数为2.43×104CFU/g,分离纯化后的8株优势菌种中,革兰氏阴性菌占87.5%,其中7株为GN-NENT(革兰氏阴性非肠遭菌)、1株为GP-ROD SB(革兰氏阳性芽孢杆菌).鉴定结果显示,8株菌种分别为:鳗鱼气单孢菌(Aeromonas encheleia)、乙酸钙不动杆菌/基因型1(Acinetobacter calcoaceticus/genospecies 1)、舒氏气单胞菌(Aeromonas schubertiiDNA group 12)、腐败希瓦氏菌B(Shewanella Putrefaciens B)、维罗纳/温和气单胞菌(Aeromonas veronii/sobria DNA group 8)、坎氏弧菌(Vibrio campbelli)、蕈状芽孢杆菌(Bacillus mycoides)和梅氏弧菌(Vibrio metschnikovii).

The distribution of cultivable organic phosphate solubilizing bacteria(OPB) and inorganic phosphate solubilizing bacteria (IPB) in the sediment of Dishui Lake and its diversion rivers was studied in 2010.Results showed that the annual average values of OPB and IPB in sediment of diversion rivers in front of the inlet gate were(2.76×10~4±1.31×10~4) and(7.19×l0~3±3.98×10~3) CFU·g~(-1), respectively;while in rivers after the gate they were(1.05×10~4±3.56×10~3) and(2.54×l0~3±8.77×10~2) CFU·g~(-1),respectively.The annual values of OPB and IPB in sediment of Dishui Lake were(6.69×10~3±2.63×10~3) and(1.66×10~3±5.83×10~2) CFU·g~(-1), respectively.In both Dishui Lake and its diversion rivers,close correlations were observed between OPB(or IPB) amount in sediment and phosphorus concentration in water.OPB or IPB amount had a significantly positive relationship with total phosphorus(TP),and the related coefficients were higher than those with bacteria,total dissolved phosphorus(TDP),and reactive phosphorus(AP).

In order to understand the status of rotifer community and the relationship with water quality, the rotifer community and water quality status in Dishui Lake river system of Shanghai were monthly studied from January to December in 2010. There were six and five sampling points in the priming river course and the lake area, respectively. 35 species of rotifers belonging to 7 families and 19 genera were identified. The dominant species were Polyarthra trigla, Trichocerca pusilla, and Anuraeopsis fissa. The uniform annual values of rotifer density and biomass in the priming river course were 821.31/L and 600.97×10-3mg/L, respectively; and the same values in the lake area were 434.98/L and 317.74×10-3mg/L, respectively. Annual means of Margalef diversity index in the priming river course and the lake area were 1.87±0.19 and 1.14±0.23, respectively. Annual means of Shannon-Wiener diversity index in the priming river course and the lake area were 1.58±0.25 and 1.21±0.76, respectively. Density, biomass, and diversity index of rotifers in the priming river course were higher than that in the lake area. Seasonally, rotifer density was significantly positively correlated with the value BOD5 and a most significantly positive correlation between rotifer biomass and BOD5 was also detected in the priming river course. There was a significantly positive correlation between the rotifer density and water temperature in the lake area and the correlation between the biomass and water temperature was most significantly positive. Horizontally, the annual mean of rotifer biomass was significantly negatively correlated with the total dissolved salt and pH, while it was significantly positively correlated with NO2-N, and also had a most significantly positive correlation with BOD5. Water quality of Dishui Lake river system was evaluated considering indicator organisms, species diversity index, and physicochemical factors of the water. The results show that lake area is in the light eutrophication level and priming river course is in the light-middle eutrophication level in the year of 2010.

In this paper,the correlation between chlorophyll a and some environmental factors including pH,temperature,dissolved oxygen,transparency,total phosphorus,total nitrogen,chemical oxygen demand and so on,was analyzed by regression statistical method,based on the investigation data in Dishui Lake from January to December in 2009.The spatial and temporal distribution characteristics of chlorophyll a were also analyzed.The results showed that the concentration of chlorophyll a ranged from 0.5 to 139.5 mg/m3 with the average value of(52.97±28.32) mg/m3.The highest concentration occurred in the period of April to June.Extremely significant differences of chlorophyll a existed between sampling site Ⅲ and VI,V,VI respectively and also between sampling site I and VI.In different sampling sites,the important environmental factors affecting chlorophyll a were different,thus different factors were chosen for the multiple stepwise regression.However,the relationship between lg(YChl.a) and lg(XTP) was positive,while that between lg(YChl.a) and lg(XTN/TP) was negative.The equations obtained were lg(YChl.a)=2.146+0.530 lg(XTP) and lg(YChl.a)=2.017-0.275 lg(XTN/TP),which were more practicable than the multiple stepwise regression equations.

Organic contents in fourteen ponds of a Litopenaeus vannamei culturing farm in Fengxian District of Shanghai were investigated during two aquaculture cycles from April to September in 2009.Total organic carbon(TOC),Chemical oxygen demand(CODMn),and Biological oxygen demand(BOD5) were analyzed respectively.The results showed that BOD5 were(8.62±3.08)mg/L and(10.47±3.87)mg/L in the two cycles of April to July and July to September respectively,CODMn were(13.09±3.98)mg/L and(16.16±6.07)mg/L,while TOC were(17.60±5.91)mg/L and(20.32±6.07)mg/L.TOC/CODMn were 1.35±0.22 and 1.32±0.30,and TOC/BOD5 were 2.10±0.44 and 2.08±0.63,while BOD5/CODMn were 0.66±0.13 and 0.65±0.11.Significant relationships lied among the three water quality parameters.Linear regression equations and related coefficients were as follows.In cycle 1:BOD5=0.4174TOC+1.2777,r=0.8022.CODMn=0.5616TOC+3.2091,r=0.8342.BOD5=0.6264CODMn+0.4209,r=0.8106.In cycle 2:BOD5=0.4764TOC+0.7902,r=0.7480.CODMn=0.7941TOC+0.0237,r=0.7962.BOD5=0.568CODMn+1.2912,r=0.8920.The results showed that the equations established among TOC,COD and BOD5 could be used to calculate the other two parameters if anyone of them had been measured so that further comparison with some water quality standards or correlated researches could be carried out,which would benefit water quality management and healthy culturing of L.vannamei.