BACKGROUND: Despite the established role of hydrogen sulfide (H2S) in the regulation of many cellular functions, the mechanisms of its effects continue to come to light, especially concerning pathogenetic significance of the gasotransmitter in chronic systemic inflammation accompanying metabolic syndrome (MS). AIM: To conduct a comparative evaluation of changes in the cytokine-producing activity of peripheral blood mononuclear leukocytes under the influence of H2S in in vitro culture of rats with an experimental model of MS and animals with normal metabolic status. METHODS: The studies were conducted on a primary culture of mononuclear leukocytes isolated from heparinized blood of laboratory animals (rats) with modeled MS by gradient centrifugation method. A series of experimental samples of cell suspension was obtained from animals with MS, and a control series was obtained from intact animals. Cells were cultured in a complete nutrient medium (based on RPMI-1640) for 24 hours in 2 variants: without and with the addition of the hydrogen sulfide donor sodium hydrosulfide (NaHS) at a final concentration of 100 μM. In the conditioned medium, the concentration of cytokines was determined using the enzyme-linked immunosorbent assay method: tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), interleukin 10 (IL-10), monocyte chemoattractant protein 1 (MCP-1), and transforming growth factor beta (TGF-â). RESULTS: Blood mononuclear leukocytes of animals with different metabolic status differently responded to addition of hydrogen sulfide donor in in vitro culturing: in healthy rats, a decrease in IL-6 production was observed (on average 1.4 times compared to the baseline secretion level, р = 0.03), whereas in the group of animals with MS, on the contrary, an increase in the concentration of IL-6 (on average, 1.4 times, р = 0.025), TNF-α (on average, 2 times, р = 0.028) and MCP-1 (on average, 1.4 times, р = 0.039) was recorded. CONCLUSION: Multidirectional action of hydrogen sulfide on the synthesis of proinflammatory cytokines and its more prominent modulating effect on cells of animals with MS suggests that the effects of H2S on leukocytes depend on in vivo metabolic conditions (the concentration of glucose, triacylglycerols, humoral inflammatory factors, adipokines, etc.), which mediate epigenetic mechanisms regulating the expression of genes responsible for the final effects of the gasotransmitter.

The use of postmortem (autopsy) material in fundamental and applied biomedical research significantly facilitates the collection of biomaterial for statistically robust sample cohorts. However, natural adaptive processes to developing cellular stress in the early postmortem period, caused by oxygen and nutrient deprivation, trigger the activation of numerous genes promoting cell survival under stress. Many of these activated pathways are also crucial for tumor cell survival in vivo, as evidenced by various transcriptomic studies. This study aimed to investigate the potential influence of postmortem interval (PMI) duration on gene expression in normal and tumor tissues. Using a model of chemically induced hepatocellular carcinoma in mouse liver, we comparatively analyzed the dynamics of transcript levels for several genes (BRCA1, BRCA2, CHEK1, CHEK2, ATM, CDK12) in paired samples of normal and tumor tissue over a 24-h PMI using RT-qPCR. In normal tissue, gene expression increased significantly, while tumor tissue demonstrated relative transcriptional stability, with no substantial changes in the studied transcript levels. A critical finding was the observed convergence of expression profiles: initial differences between the tissues were completely eliminated by 24 h PMI. This pattern developed despite formally adequate RNA quality (RQN) and the absence of clear signs of progressive autolysis in histology, indicating the insufficiency of standard quality criteria for detecting postmortem changes. These findings collectively underscore the critical importance of minimizing and controlling PMI during the biobanking of oncological samples for reliable transcriptomic research.

Iron deficiency anemia (IDA) remains a global health challenge. This study pioneers the use of humic substances (HS) as natural, biocompatible macroligands to develop safer and more effective nanoferrotherapeutics. We synthesized a series of nanoscale Fe(III) oxyhydroxide complexes stabilized by different HS, employing various solvents (ethanol, isopropanol, and acetone) and precipitation methods to isolate fractions with optimized properties. The nanocomposites were comprehensively characterized using inductively coupled plasma atomic emission spectrometry, total organic carbon analysis, X-ray diffraction, transmission electron microscopy, and Mössbauer spectroscopy. Cytotoxicity and iron bioavailability of all HS-Fe(III) formulations were assessed in Caco-2 intestinal epithelial cells. The type of HS and precipitation conditions significantly influenced the nanocomposites’ properties, yielding spherical nanoparticles (1–2 nm) of ferrihydrite or goethite. Physicochemical analysis confirmed that solvent-driven fractionation effectively tailored the nanocomposites’ size, crystallinity, and elemental composition. All HS-Fe(III) formulations demonstrated exceptional cytocompatibility, starkly contrasting the significant cytotoxicity of the reference drug Ferrum Lek®. Several complexes, particularly CHSFe-Et67, surpassed Ferrum Lek® in cellular iron uptake efficiency. We conclude that HS are a highly promising platform for developing effective and safe iron-delivery nanoferrotherapeutics, leveraging their natural polyfunctionality to enhance bioavailability and mitigate toxicity.

The level of ROS (fluorescent probe 2’,7’-dichlorodihydrofluorescein diacetate) and lipid content (fluorescent lipophilic dye Nile Red) in the peripheral blood monocyte fraction from patients with type 1 diabetes mellitus and healthy volunteers were assessed by flow cytofluorimetry. The number of CD36+ monocytes was assessed using specific antibodies. In patients with type 1 diabetes mellitus, the levels of ROS and intracellular lipids in monocytes and the number of cells expressing CD36 fatty acid translocase were elevated. These results indicate metabolic changes in the peripheral blood cells of patients with carbohydrate metabolism disorders and can be considered as possible prognostic markers for the development of type 1 diabetes mellitus complications.

The development of novel strategies for diagnosing, treating, and preventing cardiovascular diseases (CVDs) linked to metabolic syndrome and obesity presents a significant challenge for the scientific community. There is a pressing need to identify effective compounds that target the underlying pathogenic mechanisms of these disorders. Increasing knowledge about the pathogenesis of CVDs has highlighted the crucial role of perivascular adipose tissue (PVAT) in maintaining cardiovascular homeostasis. PVAT is a metabolically active endocrine organ that plays a key role in regulating blood vessel tone, endothelial function, and the growth and proliferation of vascular smooth muscle cells. However, in metabolic disorders, there is a disruption in the functional activity of PVAT cellular components and an imbalance in the production of vasoactive substances, leading to the development and progression of CVDs. This review systematically examines the morphofunctional changes in PVAT associated with metabolic syndrome and obesity, emphasizes the dysfunction of PVAT as a key pathogenetic factor in cardiovascular disease, and evaluates the potential of hydrogen sulfide (H2S) produced by PVAT as a promising vasoregulatory agent based on existing data.

Aim. To examine the pattern of morphological changes, RNA quality number, and gene expression in mouse tissues sampled at autopsy under controlled experimental conditions.Materials and methods. Balb/c mice were euthanized and subsequently subjected to necropsy at 0, 3, 12, 24, 48, and 72 hours of the postmortem period. During the first three hours following euthanasia, the mice were maintained at room temperature, after which they were transferred to a refrigerator (4 º С). Total RNA was extracted from tissue samples taken from the kidney, liver, and brain; the integrity of the RNA samples was assessed by capillary electrophoresis, and the RNA quality number (RQN) was calculated. The expression levels of Actb, Epas1, and Rps18 housekeeping genes were evaluated by real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) with original primers and probes using the TaqMan assay. The histologic examination was performed according to standard techniques.Results. Degradation of RNA extracted from mouse kidney tissues appeared to be greater than that of RNA taken from the liver. In the meantime, a negative linear correlation was observed between RQN and the duration of the postmortem interval for liver and kidney samples. In contrast, no significant changes in the RQN score were observed for brain RNA samples at any of the time points. The expression of the Epas1 and Rps18 genes was significantly decreased in mouse kidney and liver tissues. However, the level of Epas1 and Rps18 gene expression in the brain remained stable at all time points and did not exhibit a significant decrease at 72 hours after euthanasia. No obvious morphological changes were detected by the histologic examination, which does not exclude the presence of ultrastructural pathological changes.Conclusion. RQN in autopsy tissues serves as a crucial predictor of sample quality for molecular biology studies, including gene expression analysis.

Objective: One of the possible protective factors that can protect target vascular cells (endothelial, smooth muscle cells) from damage during the development of metabolic syndrome (MetS) is hydrogen sulfide (H2S). Design and method: A study was carried out in male Wistar rats weighing 250–320 g, 140 days old at the end of research. The rats from the control group were fed standard rat chow. The rats from the experimental group had a high-fat high-carbohydrate (HFHC) diet. Biochemical parameters of rat blood were determined. The concentration of H2S in the blood serum and adipose tissue of rats was determined by the photometric method at 670 nm. The expression of the H2S synthesis enzyme CSE was studied in aortic tissue samples using Western blot. Results: It was found that HFHC diet led to an increase in body weight, obesity, hyperglycemia, insulin resistance, dyslipidemia, and leptinemia in the experimental group rats. It was found that the concentration of H2S significantly decreased in the blood serum and adipose tissue of rats with MetS. The rate of production of H2S in adipose tissue of rats with MetS also decreased. In the aortic tissue of rats with MetS, there was a decrease in the amount of protein of the CSE. Conclusions: Changes in the activity of the H2S/CSE system in MetS may be additional evidence of the development of vascular dysfunction in metabolic disorders.

We propose an original strategy for metastasis prevention using a combination of three microRNAs that blocks the dedifferentiation of cancer cells in a metastatic niche owing to the downregulation of stemness genes. Transcriptome microarray analysis was applied to identify the effects of a mixture of microRNAs on the pattern of differentially expressed genes in human breast cancer cell lines. Treatment of differentiated CD44(-) cancer cells with the microRNA mixture inhibited their ability to form mammospheres in vitro. The combination of these three microRNAs encapsulated into lipid nanoparticles prevented lung metastasis in a mouse model of spontaneous metastasis. The mixture of three microRNAs (miR-195-5p/miR-520a/miR-630) holds promise for the development of an antimetastatic therapeutic that blocks tumor cell dedifferentiation, which occurs at secondary tumor sites and determines the transition of micrometastases to macrometastases.

Introduction. Biologically active substances of plant origin are the subject of study in the context of search and development of new pharmacologi-cal agents capable of influencing cholesterol metabolism in the body. The article presents the results of safety assessment, pharmacokinetics and mechanism of pharmacological activity of a new promising hypocholesterolemic agent – L-rhamnopyranosyl-6-O-methyl-galacturonan, a polysaccharide isolated from the leaves of birch (Betula pendula Roth.). Objective of the study – comprehensive study of pharmacokinetic parameters, safety and mechanisms of activity of L-rhamnopyranosyl-6-O-methyl-galacturonan in vivo and in vitro. Material and Methods. Evaluation of acute toxicity by single intragastric or intraperitoneal administration was performed on BALB/c mice and SD rats (Sprague-Dawley). To determine the effect of polysaccharide on bile acid excretion in rats with experimental hyperlipidaemia, faeces were collected for bile acid determination. Blood plasma was used in the evaluation of pharmacokinetics. Detection was performed using high-performance liquid chro-matography mass spectrometry method. To assess the sorption activity of polysaccharide, polysaccharide or a comparison drug cholestyramine was added to a solution of cholic or deoxycholic acid, unbound bile acids were quantified. Light microscopy was used to visualise polysaccharide-bile acid complexes. Results. After intragastric administration of polysaccharide at a dose of 1500 mg/kg the object of the study is practically not subjected to absorption from the digestive tract and can exert its hypolipidemic effect through effects directly in the intestinal lumen. Polysaccharide does not penetrate into organs and tissues and has no systemic action, it is completely excreted through the GI tract. According to the results of acute toxicity experiments the investigated substance can be characterised as practically non-toxic. The mechanism of hypolipidemic action of polysaccharide is associated with its ability to bind bile acids in the intestine, which is confirmed by the obtained data on the increase in the excretion of bile acids with faeces in labora-tory animals receiving polysaccharide, and the established ability of polysaccharide to bind bile acids in vitro. Conclusions. After oral administration, L-rhamnopyranosyl-6-O-methyl-galacturonan is practically not absorbed from the digestive tract, has no toxic effects, and exerts its hypolipidemic effect by binding bile acids in the intestinal lumen

An investigation was carried out on humic substances (HSs) isolated from the coal of the Kansk-Achinsk basin (Krasnoyarsk Territory, Russia). The coal HSs demonstrate the main parameters of molecular structure inherent to this class of natural compounds. An assessment was performed for the chemical, microbiological, and pharmacological safety parameters, as well as the biological efficacy. The HS sample meets the safety requirements in microbiological purity, toxic metals content (lead, cadmium, mercury, arsenic), and radionuclides. The presence of 11 essential elements was determined. The absence of general, systemic toxicity, cytotoxicity, and allergenic properties was demonstrated. The coal HS sample was classified as a Class V hazard (low danger substances). High antioxidant and antiradical activities and immunotropic and cytoprotective properties were identified. The ability of the HS to inhibit hydroxyl radicals and superoxide anion radicals was revealed. Pronounced actoprotective and nootropic activities were also demonstrated in vivo. Intragastric administration of the HS sample resulted in the improvement of physical parameters in mice as assessed by the “swim exhaustion” test. Furthermore, intragastric administration in mice with cholinergic dysfunction led to a higher ability of animals with scopolamine-induced amnesia to form conditioned reflexes. These findings suggest that the studied HS sample is a safe and effective natural substance, making it suitable for use as a dietary bioactive supplement.

Relevance. The gasotransmitter hydrogen sulphide (H2S) is awell-known signalling molecule that is involved in the regulation of awide range of cellular functions in both health and disease. Its biological effects in obesity and metabolic syndrome (MetS) have been investigated. It is apromising pharmacological target for the correction of MetS and associated diseases.The aimof this study is to investigate the role of endogenously produced H2S in the pathogenesis of metabolic disorders in experimental Met S.Materials and Methods. Ahigh-fat and high-carbohydrate diet was used to induce MS in male Wistar rats. The body and adipose tissue weights of the animals were determined. The animals body and adipose tissue weights were measured. Indicators of carbohydrate and lipid metabolism in the blood serum were determined with the use of reagent kits. Additionally, the levels of reactive oxygen species (ROS) and reduced glutathione (GSH) were analyzed in adipose tissue through photometric analysis. The concentration of H2S in blood serum and adipose tissue, as well as H2S production by adipocytes, was measured spectrophotometrically.Results and Discussion. H2S concentrations in blood serum, adipose tissue and adipocyte H2S production were found to decrease in animals with hyperglycaemia and insulin resistance. Additionally, anegative correlation was observed between the H2S content and production in the adipose tissue of rats with the mass of visceral adipose tissue. Furthermore, anegative relationship was found between the concentrations of glucose, insulin, leptin, ROS and the level of H2S in blood serum and adipose tissue. In contrast, the increase in glutathione (GSH) in adipocytes was directly correlated with the increase in hydrogen sulfide (H2S) in serum and adipose tissue cells.Conclusions. The regulatory effect of H2S on target cell function has been extensively studied. However, its role in the development and progression of MetS remains unclear. Our work demonstrates that under conditions of metabolic pathology, there is adecrease in the serum concentration of H2S and its production in adipose tissue. This decrease correlates with the development of obesity, hyperglycemia, insulinemia, leptinemia, and redox imbalance.

The aim of this review was to analyze the accumulated data on the use of mass spectrometry in diagnosing, treating, and prognosing cancer from the perspective of precision medicine. Currently, universally accepted methods for early cancer diagnosis are not available, primarily due to low molecular specificity of pathological changes at early stages of cancer development. Additionally, the existing diagnostic modalities are notably limited in sensitivity. However, early detection is imperative for selection of the most suitable cancer treatment strategy and its successful implementation. In the realm of oncology, mass spectrometry approaches show great potential for advancement and utilization. Mass spectrometry is becoming an indispensable tool in basic and applied research due to its sensitivity, specificity, and accuracy. It allows for efficient analysis of complex biological compounds, even at low concentrations. Moreover, contemporary mass spectrometry technology is capable of automating the analysis, thereby facilitating its diverse clinical applications in diagnosis, drug therapy selection, and even potential assistance to surgical oncologists in the operating room. Considering all these characteristics and advantages, mass spectrometry methods for the analysis of biological samples can be defined as some of the most promising and dynamically developing tools in precision medicine, as they are capable of providing clinically valuable information based on omics technologies, taking into account personal characteristics of the patient. Over the next decade, introduction of mass spectrometry-based methods into clinical practice based on the principles of precision medicine is expected to optimize selection of personalized treatment strategies for cancer patients and provide significant economic benefits by reducing morbidity, disability, and mortality.This comprehensive review presents the analysis of 65 scientific publications, highlighting the results of clinical and experimental studies utilizing mass spectrometry methods for diagnosing cancer, investigating the underlying mechanisms of disease development, and evaluating the efficacy of therapeutic interventions. The review encompasses original articles published from January 1, 2018 to November 30, 2023. The majority of studies back the potential of mass spectrometry as a valuable tool for cancer diagnosis and treatment monitoring. Broadening application of mass spectrometry techniques in the field of oncology holds significant promise and represents a relevant area for future research.

Aim. To develop a new method to determine the viability of Opisthorchis felineus in vitro using the MTS reagent and to evaluate its applicability for analyzing the efficacy of anthelmintic agents in the treatment of opisthorchiasis.Materials and methods. Golden hamsters were used to create a model of O. felineus infection. The animals were infected with metacercariae obtained from fish of the Cyprinidae family. Three months after infection, adult parasites were extracted from the hepatobiliary system. Their viability was assessed using the motility scale and a new method based on the modified MTS test protocol. To account for differences between the size and number of adult parasite cells, the results were normalized with respect to protein content. To evaluate the feasibility of the new approach in the study of pharmacological activity against opisthorchiasis, the viability of adult parasites in the presence of praziquantel was tested.Results. During incubation of adult flukes in a medium with the addition of the MTS reagent, colored watersoluble formazan was accumulated. Thermal inactivation of parasites significantly decreased the production of this compound. Since the studied adult parasites differed in size and number of cells, the obtained data on their viability were normalized to protein content. The results correlated with the data on parasite viability obtained by the traditional method using the motility scale. Evaluation of praziquantel efficacy at different concentrations using two independent methods (the MTS test and the motility scale) showed that the results of the MTS test were consistent with literature data and comparable with the results obtained using the motility scale.Conclusion. A new method for in vitro evaluation of anti-opisthorchiasis activity of drugs was developed. It is based on the assessment of water-soluble formazan production by adult O. felineus flukes in the culture medium using the MTS reagent for screening anti-opisthorchiasis activity of new anthelmintic drugs.

Phenotypic characteristics of alveolar macrophages in the bronchoalveolar lavage fluid as well as their ability to acquire the M1 and M2 phenotypes during in vitro culturing with reprogramming factors were studied in rats with modeled diet-induced metabolic syndrome. A decrease in the number of alveolar macrophages with the M1 phenotype was found in animals with metabolic syndrome. The factors of metabolic syndrome do not affect phenotypic plasticity of cells in culture, but under the action of M2 reprogramming factors, the cells demonstrate a wide range of phenotypic plasticity by the CD80 and CD206 markers. The consistently high level of production of IL-6 and IL-10 by macrophages during culturing under different conditions indicates functional rigidity of the cells, which is probably a consequence of in vivo predetermined functional phenotype of these cells against the background of metabolic disorders.

Type 1 diabetes mellitus was modeled in Wistar rats by intraperitoneal injection of streptozotocin (25 mg/kg for 5 days), which led to the appearance of the main symptoms of insulin-dependent diabetes. In peripheral blood mononuclear cells isolated by centrifugation on a Ficoll density gradient, the production of ROS and the level of intracellular lipids were evaluated by flow cytofluorimetry. In rats with type 1 diabetes mellitus, an increase in ROS levels in isolated peripheral blood monocytes, but not in the lymphocytic fraction was revealed. Incubation of isolated monocytes in a medium containing 1 mM oleic acid led to a 1.5-fold increase of intracellular lipid levels. After incubation of the lymphocyte fraction in this medium, no differences from the control were revealed. Disorders of carbohydrate and lipid metabolism in type 1 diabetes mellitus leading to an increase of free fatty acids and ROS levels can be detected ex vivo in isolated peripheral blood mononuclear cells.

The aim of the study was to assess the level of CD68-positive macrophages in adipose tissue in rats with of diet-induced MetS against the background of changes in the concentration of pro-inflammatory cytokines. In animals fed a high-fat and high-carbohydrate diet, there was an increase in body weight, obesity, metabolic disorders, a high concentration of cytokines IL-10 and IL-10, an increase in the content of CD68 macrophages in adipose tissue was observed.

Physicochemical and pharmacological properties of the humic substances from coal of the Kansk-Achinsk coal basin, the Pereyaslovskoe deposit, have been studied. The presence of polycyclic aromatic structures and aliphatic fragments with various functional groups as substituents (carboxyl, carbonyl and quinoid, phenolic, alcoholic, ether and ester, amino and amide) was established by means of UV, IR, 13C NMR spectroscopy, fluor-imetry, and elemental analysis. It has been determined by HPLC that coal humic substances are highly hydro-philic polydisperse biopolymers with medium molecular weights. According to the indicator of microbiological purity (category 3B), the content of microorganisms does not exceed the indicators required by the State Pharmacopeia of the Russian Federation (SPh XIV edition). The content of radionuclides, toxic metals (lead, cadmium, mercury, arsenic) corresponds to the norms (according to SPh XIV edition). The presence of 11 essential elements has been established. The studied humic substances belong to the V class of hazard (low-hazard sub-stances, according to GOST 32644-2014), do not have allergising properties and cytotoxic effect within a broad concentration range. Antioxidant, immunotropic, cytoprotective properties were established in the studies of specific pharmacological activity with various experimental models. The ability to inhibit free radicals such as hydroxyl radicals (HO.) and superoxide-anion radical (O2-. should be stressed, as these radicals are able to by-pass the endogenous antioxidant defense systems of the body. It is concluded that the studied humic substances of the Kansk-Achinsk coal basin, the Pereyaslovskoe deposit, are safe and effective biologically active sub-stances of natural origin for use as a promising biologically active substance in the food and pharmaceutical industries.

The current article describes the biological activity of new biomaterials combining the “green” properties of humic substances (HSs) and silver nanoparticles. The aim is to investigate the antioxidant activity (AOA) of HS matrices (macroligands) and AgNPs stabilized with humic macroligands (HS-AgNPs). The unique chemical feature of HSs makes them very promising ligands (matrices) for AgNP stabilization. HSs have previously been shown to exert many pharmacological effects mediated by their AOA. AgNPs stabilized with HS showed a pronounced ability to bind to reactive oxygen species (ROS) in the test with ABTS. Also, higher AOA was observed for HS-AgNPs as compared to the HS matrices. In vitro cytotoxicity studies have shown that the stabilization of AgNPs with the HS matrices reduces the cytotoxicity of AgNPs. As a result of in vitro experiments with the use of 2,7-dichlorodihydrofluorescein diacetate (DCFDA), it was found that all HS materials tested and the HS-AgNPs did not exhibit prooxidant effects. Moreover, more pronounced AOA was shown for HS-AgNP samples as compared to the original HS matrices. Two putative mechanisms of the pronounced AOA of the tested compositions are proposed: firstly, the pronounced ability of HSs to inactivate ROS and, secondly, the large surface area and surface-to-volume ratio of HS-AgNPs, which facilitate electron transfer and mitigate kinetic barriers to the reduction reaction. As a result, the antioxidant properties of the tested HS-AgNPs might be of particular interest for biomedical applications aimed at inhibiting the growth of bacteria and viruses and the healing of purulent wounds.

In male Syrian hamsters fed a synthetic high-fat diet enriched with cholesterol (0.3%), administration of a polysaccharide from birch leaves L-rhamnopyranosyl-6-O-methyl-D-galacturonan (3 g/100 g of diet) resulted in a decrease in total cholesterol levels, mainly due to the LDL fraction, triglycerides, and bile acids in blood serum; the content of triglycerides and cholesterol in the liver also decreased, while excretion of bile acids with feces increased. Thus, the lipid-lowering effect of L-rhamnopyranosyl-6-O-methyl-D-galacturonan is related to its ability to bind bile acids in the intestine and interrupt their enterohepatic circulation.

The aim was to investigate wound-healing properties of zinc-containing biocomposites based on humic ligands (humic substance (HS) – Zn) in the in vivo experiment on the aseptic wound model and to evaluate their resorptive properties. Materials and methods. The objects of the study were 5 samples of HS-Zn in the form of complex salts comprising fine black powders synthesized in the Laboratory for Natural Humic Systems of the Faculty of Chemistry at Moscow State University. The wound-healing effect of the substances was studied on 70 male Wistar rats using a traumatic model of an excisional aseptic skin wound. The degree of affected skin healing was evaluated during 21 days by the planimetric method. The resorptive properties of the HS-Zn samples were studied by inductively coupled plasma mass spectrometry (ICP-MS) in the biomaterial (blood serum, fur, skin from the wound surface). Results. It was found that course application of zinc-containing HS-Zn biocomposites to the wound surface led to a decrease in the wound area in comparison with ZnSO 4 with the equivalent concentration of elemental Zn (1.67 mg/ ml). Two samples FA-Zn and Peat1-Zn showed the most pronounced regenerating effect. We noted an increase in Zn level in the tested skin samples from the wound area, in fur, and in the blood serum, which indicates the resorptive effect of zinc-containing HS-Zn biocomposites during course application; however, the parameters did not exceed limiting permissible concentrations. The correlation between the tested samples was not equal, which indicates a significant impact of the initial HS matrix on the Zn bioavailability. Conclusion. The observed reparative effect of zinc and HS complexes in the context of their low toxicity is of interest for further study to develop effective wound-healing preparations.