The high-current fast electron beams generated in high-intensity laser-solid interactions require the onset of a balancing return current in order to propagate in the target material. Such a system of counter-streaming electron currents is unstable to a variety of instabilities such as the current-filamentation instability and the two-stream instability. An experimental study aimed at investigating the role of instabilities in a system of symmetrical counter-propagating fast electron beams is presented here for the first time. The fast electron beams are generated by double-sided laser-irradiation of a layered target foil at laser intensities above 1019 W/cm2. High-resolution X-ray spectroscopy of the emission from the central Ti layer shows that locally enhanced energy deposition is indeed achieved in the case of counter-propagating fast electron beams.

Alterations in intracellular cardiomyocyte calcium handling have a key role in initiating and sustaining arrhythmias. Arrhythmogenic calcium leak from sarcoplasmic reticulum (SR) can be attributed to all means by which calcium exits the SR store in an abnormal fashion. Abnormal SR calcium exit maymanifest as intracellular Ca2+ sparks and/or Ca2+ waves. Ca2+ signaling in arrhythmogenesis has been mainly studied in isolated cardiomyocytes and given that the extracellular matrix influences both Ca2+ and membrane potential dynamics in the intact heart and underlies environmentally mediated changes, understanding how Ca2+ and voltage are regulated in the intact heart will represent a tremendous advancement in the understanding of arrhythmogenic mechanisms. Using novel high-speed multiphoton microscopy techinques, such as multispot and random access, we investigated animal models with inherited and acquired arrhythmias to assess the role of Ca2+ and voltage signals as arrhythmia triggers in cell and subcellular components of the intact heart and correlate these with electrophysiology.

Alterations in intracellular cardiomyocyte calcium handling have a key role in initiating and sustaining arrhythmias. Arrhythmogenic calcium leak from sarcoplasmic reticulum (SR) can be attributed to all means by which calcium exits the SR store in an abnormal fashion. Abnormal SR calcium exit maymanifest as intracellular Ca2+ sparks and/or Ca2+ waves. Ca2+ signaling in arrhythmogenesis has been mainly studied in isolated cardiomyocytes and given that the extracellular matrix influences both Ca2+ and membrane potential dynamics in the intact heart and underlies environmentally mediated changes, understanding how Ca2+ and voltage are regulated in the intact heart will represent a tremendous advancement in the understanding of arrhythmogenic mechanisms. Using novel high-speed multiphoton microscopy techinques, such as multispot and random access, we investigated animal models with inherited and acquired arrhythmias to assess the role of Ca2+ and voltage signals as arrhythmia triggers in cell and subcellular components of the intact heart and correlate these with electrophysiology.

We report on the development of radiation and electron sources based on laser-plasma acceleration for biomedical and nuclear applications, using both the table top TW laser at ILIL and the 220 TW FLAME laser system at LNF. We use the ILIL laser to produce wakefield electrons in a self-focusing dominated regime in a mm scale gas-jet to generate large, uniform beams of MeV electrons for electron radiography and radiobiology applications. This acceleration regime is described in detail and key parameters are given to establish reproducible and reliable operation of this source. We use the FLAME laser to drive laser-plasma acceleration in a cm-scale gas target to obtain stable production of >100 MeV range electrons to drive a Thomson scattering ɣ-ray source for nuclear applications.

We report on the characterization and use of a Multispot Multiphoton Microscope, to investigate calcium dynamics at intracellular level. Time resolution of a few milliseconds, even in full frame images at 512×512 pixels, is achieved, in order to get the most information on the evolution and propagation of ionic calcium waves across adjacent cells in an intact cardiac tissue.

Isochoric heating of solid-density matter up to a few tens of eV is of interest for investigating astrophysical or inertial fusion scenarios. Such ultra-fast heating can be achieved via the energy deposition of short-pulse laser generated electrons. Here, we report on experimental measurements of this process by means of time- and space-resolved optical interferometry. Our results are found in reasonable agreement with a simple numerical model of fast electron-induced heating.

Shock ignition (SI) is an appealing approach in the inertial confinement scenario for the ignition and burn of a pre-compressed fusion pellet. In this scheme, a strong converging shock is launched by laser irradiation at an intensity I lambda(2) >10(15) W cm(-2) mu m(2) at the end of the compression phase. In this intensity regime, laser-plasma interactions are characterized by the onset of a variety of instabilities, including stimulated Raman scattering, Brillouin scattering and the two plasmon decay, accompanied by the generation of a population of fast electrons. The effect of the fast electrons on the efficiency of the shock wave production is investigated in a series of dedicated experiments at the Prague Asterix Laser Facility (PALS).We study the laser-plasma coupling in a SI relevant regime in a planar geometry by creating an extended preformed plasma with a laser beam at similar to 7 x 10(13) W cm(-2) (250 ps, 1315 nm). A strong shock is launched by irradiation with a second laser beam at intensities in the range 10(15)-10(16) W cm(-2) (250 ps, 438 nm) at various delays with respect to the first beam. The pre-plasma is characterized using x-ray spectroscopy, ion diagnostics and interferometry. Spectroscopy and calorimetry of the backscattered radiation is performed in the spectral range 250-850 nm, including (3/2)omega, omega and omega/2 emission. The fast electron production is characterized through spectroscopy and imaging of the K alpha emission. Information on the shock pressure is obtained using shock breakout chronometry and measurements of the craters produced by the shock in a massive target.Preliminary results show that the backscattered energy is in the range 3-15%, mainly due to backscattered light at the laser wavelength (438 nm), which increases with increasing the delay between the two laser beams. The values of the peak shock pressures inferred from the shock breakout times are lower than expected from 2D numerical simulations. The same simulations reveal that the 2D effects play a major role in these experiments, with the laser spot size comparable with the distance between critical and ablation layers.

A new era of laser based plasma accelerators is emerging following the commissioning of many high power laser facilities around the world. Extremely short (tens of fs) laser pulses with energy of multi-joules level are available at these newly built facilities. Here we describe the new 220 TW FLAME facility. In particular we discuss the laser system general layout, the main measurements on the laser pulse parameters, the underground target area. Finally we give an overview of the first results of the Self-Injection Test Experiment (SITE), obtained at a low laser energy. This initial low laser energy experimental campaign was necessary for the validation of the radio-protection shielding (Esposito, 2011 [1]) we discuss here. With respect to our preliminary configuration, with a pulse duration of 30 fs and a focusing optic of F/15, we discuss here the minimum laser energy requirements for electron acceleration and the forward transmitted optical radiation.

Spectral modification of an intense (≥1018Wcm−2) laser pulse propagating in a helium gas target was studied using Thomson scattering diagnostic. The spectra recorded at 90° to the laser propagation and polarization direction show a significant modulation and modification of the incident laser pulse during its interaction and propagation through the helium gas target. Simulations suggest that such a modulation in the spectrum of the laser pulse could arise due to its interaction with the self excited plasma waves.

Over the past few years, a new X-ray diagnostic technique has been developed by the group of the Intense Laser Irradiation Laboratory of the CNR in Pisa. Here we give a brief description of this new diagnostic tool, which basically allows X-ray spectroscopy of laser-produced plasmas to be performed over a broad photon energy range with a simultaneous 2D spatial resolution. An outline of recent results will be also given in order to assess the diagnostic capabilities.

A review of the proton radiography technique will be presented. This technique employs laser-accelerated laminar bunches of protons to diagnose the temporal and spatial characteristic of the electric and magnetic fields generated during high-intensity laser-plasma interactions. The remarkable temporal and spatial resolution that this technique can achieve (of the order of a picosecond and a few microns respectively) candidates this technique as the preferrable one, if compared to other techniques, to probe high intensity laser-matterinteractions.

The dynamics of the focusing of laser-driven ion beams produced from concave solid targets was studied. Most of the ion beam energy is observed to converge at the center of the cylindrical targets with a spot diameter of 30  μm, which can be very beneficial for applications requiring high beam energy densities. Also, unbalanced laser irradiation does not compromise the focusability of the beam. However, significant filamentation occurs during the focusing, potentially limiting the localization of the energy deposition region by these beams at focus. These effects could impact the applicability of such high-energy density beams for applications, e.g., in proton-driven fast ignition.

An increasing number of high-power laser laboratories are being established with the aim of developing laser-plasma accelerators. Here a short overview is given on the preliminary results obtained in the self-injection test experiment as a part of the commissioning phase of the 220 TW FLAME laser facility. For laser energies on target below 1 J and pulse durations around 30 fs, accelerated electron bunches had an energy up the hundred MeV range, showing a significant degree of collimation below 10 mrad, in agreement with similar published results and confirming the expected laser performance at this energetic level. A correlation between electron collimation and upshift on laser transmitted light was also observed.

The dynamics of magnetic fields with an amplitude of several tens of megagauss, generated at both sides of a solid target irradiated with a high-intensity (~10(19) W/cm(2)) picosecond laser pulse, has been spatially and temporally resolved using a proton imaging technique. The amplitude of the magnetic fields is sufficiently large to have a constraining effect on the radial expansion of the plasma sheath at the target surfaces. These results, supported by numerical simulations and simple analytical modeling, may have implications for ion acceleration driven by the plasma sheath at the rear side of the target as well as for the laboratory study of self-collimated high-energy plasma jets.

We report on the characterization and use of a Multispot Multiphoton Microscope, to investigate calcium dy- namics at intracellular level. We apply this technique to obtain a time resolution of a few milliseconds, even in full frame images at 512x512 pixels, in order to get the most information on the evolution and propagation of ionic calcium waves across adjacent cells in an intact cardiac tissue. Further we report on the progress of development of a Random Access microscope for very high speed all optical electrophysiological signal acquisition in cell networks. Our study opens the way to the investigation of arrhythmogenic disease in animal models at cellular level.

The advance in laser–plasma acceleration techniques pushes the regime of the resulting accelerated particles to higher energies and intensities. In particular, the upcoming experiments with the 250 TW laser at the FLAME facility of the INFN Laboratori Nazionali di Frascati, will enter the GeV regime with more than 100 pC of electrons. At the current status of understanding of the acceleration mechanism, relatively large angular and energy spreads are expected. There is therefore the need for developing a device capable to measure the energy of electrons over three orders of magnitude (few MeV to few GeV), with still unknown angular divergences. Within the PlasmonX experiment at FLAME, a spectrometer is being constructed to perform these measurements. It is made of an electro-magnet and a screen made of scintillating fibers for the measurement of the trajectories of the particles. The large range of operation, the huge number of particles and the need to focus the divergence, present challenges in the design and construction of such a device. We present the design considerations for this spectrometer that lead to the use of scintillating fibers, multichannel photo-multipliers and a multiplexing electronics, a combination which is innovative in the field. We also present the experimental results obtained with a high intensity electron beam performed on a prototype at the LNF beam test facility.

The experimental evidence of the correlation between the initial electron density of the plasma and electromagnetic soliton excitation at the wake of an intense (1019 W/cm2) and short (1 ps) laser pulse is presented. The spatial distribution of the solitons, together with their late time evolution into post-solitons, is found to be dependent upon the background plasma parameters, in agreement with published analytical and numerical findings. The measured temporal evolution and electrostatic field distribution of the structures are consistent with their late time evolution and the occurrence of multiple merging of neighboring post-solitons.

A detailed knowledge of the physical phenomena underlying the transport of fast electrons generated in high-intensity laser-matter interactions is of fundamental importance for the fast ignition scheme for inertial confinement fusion. The fast electron currents largely exceed the Alfven limit, therefore a balancing return current is required to support the propagation of the fast electron beam in the target medium. An experimental study aimed at investigating the role of the return current on the dynamics of the fast electron beam was carried out with the Vulcan Petawatt beam. Two counter-propagating electron beams were generated by double-sided irradiation of a layered target containing a 5 micron thick Ti layer. Information on the energy coupling of the fast electron beam to the Ti layer was retrieved through X-ray measurements. In particular, high-resolution X-ray spectroscopy of the Ti emission lines was performed in the spectral range from 4.4 to 5.1 keV including the Lya, the Hea and the Ka line. Spectra were acquired for double-sided irradiation with different timings between the two laser beams as well as for single-sided irradiation. The recorded spectra indicate a higher target temperature for a precise timing between the two beams in agreement with simulation results.

Laser-plasma acceleration can provide acceleration gradients which are thousands of times stronger than conventional electron accelerators. The laser propagation length is a crucial parameter that must be extended to achieve high-energy electrons. Here, we show that color images of the laser-plasma interaction region taken from the direction perpendicular to the polarization plane are a powerful tool to discriminate between Thomson scattering and plasma self-emission, leading to a precise measurement of the propagation length.