Meat, the muscle protein of animals, is a desirable component of a diet/meal supplying vitamins, proteins, and minerals. Meat can be whole tissue meat, can be ground, or can be in a processed form such as sausages, salamis or dried using salt or smoking for preservation. Cooking enhances the flavor and increases the availability of nutrients. Putative health issues for meat are paralleled by excessive consumption of all foods. While the risk of bowel cancer is low it may be increased through eating meat – the effect is small when compared with other cancers and reduction of life expectancy through poverty – vegetables confer a protective effect. Nitrite is a preservative in meat products with levels in processed meat being actually lower than that in saliva and many vegetables such as lettuce and celery. Cumulative greenhouse gas production from ruminants is minimal through the recycling of agricultural methane – taking 60 years to reach a steady state before it is broken down to carbon dioxide to be incorporated by plants via photosynthesis. Good animal welfare ensures high production with minimal disease and improves meat quality through low stress.

The M. longissimus from lambs electrically stimulated at 15 min post-mortem were removed after grading, wrapped in polythene film and held at 4 (n=6), 7 (n=6), 15 (n=6, n=8) and 35°C (n=6), until rigor mortis then aged at 15°C for 0, 4, 24 and 72 h post-rigor. Centrifuged free water increased exponentially, and bound water, dry matter and shear force decreased exponentially over time. Decreases in shear force and increases in free water were closely related (r(2)=0.52) and were unaffected by pre-rigor temperatures.

Four groups of carcasses of eighty grain fed low stressed steers (~ 12 months old, 240 kg carcass weight) received no stimulation or had low voltage stimulation for 15, 45 and 90 s. M. longissimus was aged for 2 and 14 days at 2 °C. Shear force, sarcomere length, water holding capacity, purge, muscle fibre detachment, sarcomere breaks and colour shelf life over 7 days was measured. Both 45 s and 90 s stimulated samples completed rigor above 35 °C and 15 s stimulated and non-stimulated samples below 35 °C. The greatest muscle fibre detachment occurred for 15 s stimulated samples which were the most tender at all times with non-stimulated samples toughest at 2 days of ageing and all samples being tender at 14 days of ageing. The 45 and 90 s stimulated samples consistently exhibited a brighter red colour at 2 days of ageing with no differences in colour intensity for the samples aged 14 days.

This study included simultaneously measured pre and post-rigor meat quality indicators and attributes, using near infrared (NIR) spectroscopy and reference methods, to understand the temporal, biochemical and structural factors that influence beef quality and use this knowledge to build calibrations for measurement of meat quality using NIR. Eighty beef M. longissimus lumborum (LL) were measured from early pre-rigor (pH, glycogen concentration, and temperature) through to completion of post-rigor ageing (pH, shear force, and water holding capacity (WHC)). To create a range in the attributes, the LLs were subjected to various pre-rigor treatments, including electrical stimulation, restraint wrapping and cooling temperature (5°C, 15°C, or 35°C). Alongside the reference quality measurements and throughout the pre and post-rigor period, the LLs were measured with a diode array NIR spectroscopy system. NIR reflectance measurements were shown to be correlated to reference measurements of pre and post-rigor pH (R(validation)(2)=0.84), pre-rigor glycogen content (R(validation)(2)=0.70), post-rigor shear force (R(validation)(2)=0.58) and post-rigor WHC (R(validation)(2)=0.68). The shape of the NIR and reference plots over time and the ability of NIR to correctly measure differences in treatments indicated that NIR was not merely measuring changes that occur over time, but was measuring specific biochemical and physical changes, most likely changes in glycolytic metabolites, muscle shortening and/or proteolysis. Accounting for the reference method variance showed NIR measurement accuracy to be as good as or slightly better than that of the reference method.

A three factorial experimental design involving electrical stimulation (ES/NES), wrapping (wrapped/unwrapped) and pre rigor temperature (15 °C or 35 °C) was applied to 70 beef M. longissimus lumborum muscles to obtain a wide variation in shear force and drip loss. The shear force of all treatment groups decreased during ageing. As anticipated, wrapping and electrical stimulation had positive effects on shear force. However, high pre rigor temperature (35 °C) did not result in higher shear force values if the muscles were electrically stimulated, wrapped or both. The results suggested that electrical stimulation protects against the negative effects of high pre rigor temperatures. The drip loss of all treatment groups increased during ageing in a manner that was unrelated to treatment but was correlated to tenderness (r2 = 0.70; p < 0.0001). It was concluded that the application of electrical stimulation, whatever the pre rigor temperature, protects beef from toughening through the prevention of rigor shortening and the avoidance of inhibition of ageing enzymes.