In this study, we conducted a case-control study to explore the association between rs1550117 A>G variant of DNMT3A gene promoter and non-small cell lung cancer (NSCLC) susceptibility in a Han Chinese population. The genotyping of rs1550117 A>G variant was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and confirmed by sequencing. Allele G of rs1550117 was associated with an increased risk of NSCLC. Moreover, individuals carrying the GG genotypes had a higher risk to develop NSCLC than the AA and GA genotype carriers. Further stratified analysis showed that rs1550117 A>G was significantly related to age (> 60 years), male, smoking and drinking. In vivo detection of DNMT3A mRNA levels in NSCLC tissues and in vitro luciferase assays consistently showed that the allele G significantly decreased DNMT3A transcription. Additional functional analysis revealed that the increased binding affinity of transcription repressor SP1, which was associated with allele G of rs1550117, led to the significant decreased expression of DNMT3A. Collectively, our results propose a suppression role of DNMT3A in NSCLC development and emphasize the dual roles of DNMT3A in tumorigenesis.

To study the influence of 60Co γ ray irradiation dose on bovine serum component,as a single component model,BSA solution was irradiated by 0~50 kGy respectively and the protein content,secondary structure molecular and morphology were determined. The results showed that the BSA was mainly composed of 10 proteins and the molecular weights were 25~214 kDa,of which the protein content of 55 kDa was the largest one. The protein content was decreased with the increasing of irradiation dose and the content of 10 proteins were degraded after irradiated 50 kGy. The UV-Vis (UV -Visible Spectrophotometer) of BSA appeared two absorption peaks at 210 nm and 280 nm,respectively. The height of two peaks were decreased with the dose increasing and 1 280 nm-peak disappeared after the BSA was irradiated by 30 kGy. The secondary structure content of α-helix,β-fold & β-corner and random coil from BSA were 40.8%,37.3% and 21.9%. The α-helix content decreased with the dose increasing and the other structural content increased with the dose increading. Amide I band of infrared absorption peak of bovine serum albumin shifted from 1 650.8 cm-1 to 1 655.5 cm-1. The BSA had a smooth surface microstructure before irradiation and changed into a loose sheet after irradiaton. Thus,the main effect region of the radical irradiation was α-helix of BSA,subsequently led the increasing of β-fold,β-turn and random coil increases,finally the protein turned from the ordered state into a disordered state,the greater the dose,the greater the degree of reaction.

探讨了黑花生(Arachis hypogaea Linn.)糕配方及工艺参数,旨在为其进一步工业生产提供依据.采用单因素试验对黑花生糕的配方及蒸煮时间等工艺参数进行研究.结果表明,影响黑花生糕感官得分的主次因素为木糖醇量＞蒸煮时问＞黑花生粉量＞糯米粉量;在木糖醇14％、黑花生粉26％、糯米粉30％、蒸煮5 min条件下制备的黑花生糕口感好、品质优良,适合进行工业生产.