目的:研究食管鳞状细胞癌患者实施胸腔镜联合食管癌根治术的临床价值.方法:纳入本院(2016年04月-2018年04月)接收的食管鳞状细胞癌患者(n=84)作为研究对象,以随机表为基准,分为实验组(n=42,实施胸腔镜联合食管癌根治术治疗)、对照组(n=42,实施开胸手术治疗),对84例患者并发症发生率、住院时间评估.结果:并发症发生率:实验组并发症发生率低于对照组,P<0.05.住院时间:实验组住院时间低于对照组,P<0.05.结论:食管鳞状细胞癌患者实施胸腔镜联合食管癌根治术的临床价值显著,既可缩短住院时间,又可降低并发症发生率,值得借鉴.

目的:探究甲状腺全切除术对甲状腺微小癌并发症及生活质量的影响.方法:选取我院自2017年6月至2018年6月收治的100例甲状腺微小癌患者作为本次研究的主要对象,以简单随机法分为对照组与实验组各50例,对照组予以甲状腺患侧腺叶全切除术治疗,实验组予以甲状腺全切除术治疗,实验结束后,对两组病人的手术时间及住院时间、术中出血量、并发症发生率进行观察对比.结果:与对照组比较发现,实验组手术时间及住院时间、术中出血量、治疗效果评分均优于对照组,两组比较差异明显,具有统计学意义(P<0.05),与此同时实验组并发症发生率为6.7％,明显低于对照组的26.7％,两组比较具有统计学意义(P<0.05).结论:甲状腺全切除术对甲状腺微小癌并发症有一定的应用价值且有助于减少患者的手术时间及住院时间、术中出血量,值得推荐.

Objective To observe the effects of 9L/IL-18 on the expressions of CD80,CD86,MHCⅠand MHCⅡin tumour tissues of rats transfected with IL-18, and to explore the anti-tumour effects of IL-18.Methods The F344 rats were randomly divided into three groups ,with 7 rats in each group,and these rats were inoculated with 9L/IL-18 cells,9L/LXSN cells,9L cells,respectively.The expression levels of CD80,CD86,MHCⅠ,MHCⅡin tumor tissues were detected by flow cytometry.Results The expression levels of CD80,CD86,MHCⅠin tumor tissues of rats inoculated with 9L/IL-18 cells were significantly higher than those in tumor tissues of rats inoculated with 9L/LXSN cells and 9L cells ( P <0.05).However there were no significant differences in the expression levels of CD 80,CD86,MHCⅠbetween 9L/LXSN cell inoculation group and 9L cell inoculation group ( P >0.05).There were no significant differences in the expression levels of MHCⅡ among the three groups ( P >0 .05 ) .Conclusion IL-18 may promote the differentiation and maturation of dendritic cells by increasing the expressions of CD80, CD86, MHCⅠon the surface of tumor cells,thereby,which may activate T cells,enhance the antigent activity of tumour cells to improve the effects of immune surveillance and to produce anti -tumor effects .

Objective:To assess the effect on the expression of TGF -βand Fgf2 of the exogenous IL -18 gene on the biological characteristics of rat 9L glioma cells.Methods:The cell suspension of 9L/IL -18,9L/LXSN and 9L cells were inoculated into the brain of F344 rat,detct the level of TGF -βand Fgf2 by enzymelinked immunosorbent assay and RT -PCR.Results:The level of TGF -βin the peripheral blood of rats inoculated 9L/IL -18 was weak-end to that inoculated 9L/LXSN and 9L(P <0.05).Compared to 9L/LXSN and 9L cells inoculated rat,the expression of Fgf2 were decreased in the tumor tissue of rat inoculated 9L/IL -18.Conclusion:IL -18 could decrease the levelof TGF -βand Fgf2.

Objective To analyze the effects of IL-18 transfection on the 9L glioma growth in vitro and the apoptosis of tumor cells.Methods 9L/IL-18,9L/LXSN and 9L cells were inoculated into the brain of F344 rat to establish the animal models by the stereotactic technique.The tumorigenicity of different cells was observed, and then the tumorigenic rates were calculated. The cell apoptosis was detected by TUNEL.The apoptosis rate of tumor cells in each group was calculated.The expression of Bax and Bcl-2 in tumor tissues was detected by RT-PCR and Westen Blot. Results The rate of tumor formation in each groups was 100%;TUNEL assay showed that a large number of apoptotic cells were detected in the tumor tissues of the 9L/IL-18 cell group,in which the apoptotic rate was (22.2±1.02)%,significantly higher than that in 9L/LXSN (5.7±0.32)% and 9L (6.4±0.51)% groups (P<0.05);the expression of Bax in the tumor tissues of inoculated 9L/IL-18 cell group was higher than those of the other groups, and the expression of Bcl-2 was lower than those of the other groups.Conclusion By up regulating the expression of Bax,down regulating the expression of Bcl-2,transfected exogenously IL-18 gene can pro-mote the apoptosis of tumor cells and play an anti-tumor role.

Objective:To study the effect of vascular endothelial growth factor(VEGF) in resisting infection of skin flap.Mtehods: Randomized flaps 6cm×4cm were created on the back of 60 Sprague-Dawley rats.The treatment group(n=30) received VEGF 1.0μg/flap during the operation.The control group(n=30) received equal quantity normal saline.After operation,Pseudomonas aeruginosa bacteria was inoculated immediately.Results: Immunohistochemical staining of basic fibroblast growth factor(bFGF): At the third postoperative day,there was a significant increase in bFGF expression in the middle part and the distal part of flap tissue in the VEGF treated group as compared to the corresponding portions in the control group(P0.01).Succinate dehydrogenase(SDH) level: At the forth postoperative day,the level of succinate dehydrogenase in the middle part and the distal part of flap tissue in the VEGF treated group were significantly increased(P0.01).Transmission electron microscopy(TEM) studies: the structures and functions of fibroblast and vascular endothelial cell in the VEGF treated group were better than those in the control group.Interleukin-1β expression in the control group flap significantly increased at the eleventh postoperative day compared with treatment group(P0.01).Vascular density in the treatment group significantly increased at the eleventh postoperative day compared with control group(P0.01).Conclusion: Vascular endothelial growth factor(VEGF) might improve the flap micro-circulation,accelerate the vascularization of the flap,protect flaps from ischemia and play an important role in improvement flap survival and resisting infection.

The objective of this study was to discuss the diagnostic values of color doppler combined with molybdenum target radiography for occult breast cancer.The clinical data of 1 293 patients examined with molybdenum target radiography and color doppler from 11-01-2005 to 11-31-2006 in in Tangshan People's Hospital (n=836) and Yutian People's Hsopital (n=457) were analyzed.Among all of 1 293 patients,11 of them all were considered breast cancer examined with molybdenum target radiography,6 patients were breast cancer approved by biopsy and 4 patients were put into practice lymph node biopsy by color doppler.Color doppler combined with molybdenum target radiography is an important method to discover and diagnose occult breast cancer.

直肠癌手术方式颇多,经腹前切除术为目前最理想的保肛术式,但术后吻合口漏是其较常见的并发症.该并发症的预防与治疗是提高疗效的重要环节.本文报告我在北京肿瘤医院进修期间总结该院2003年1月～2005 年12 月直肠癌经腹前切除术729 例,并发吻合口漏48 例,发生率为6.5%.

OBJECTIVE To evaluate the Chymotrypsin and Hyaluronidase in prevention of incision adhesion in thyroid surgery.METHODS Patients underwent thyroid surgery were randomly divided into 4 groups: Chymotrypsin group,Hyaluronidase group,Normal solution group and Control group.The incision were observed at 2,4,6,12 months after operation.RESULTS The incision adhesion incidences in the first 3 groups were significantly different compared with that in control group(P 0.05).CONCLUSION The medicine can decrease the incision adhesion incidence in thyroid surgery.