The Paleocene-Eocene thermal maximum (PETM, similar to 56 Ma) is marked by a massive and rapid rise in atmospheric CO2 and similar to 5 degrees C of global warming. It is globally characterized by a negative carbon isotope excursion (CIE), and, at least locally, is preceded by a pre-onset excursion (POE). We present palynological and bioclimatic analyses from stratigraphically expanded marginal marine sediment sections from the eastern United States. Late Paleocene forests were dominated by needle-leaved gymnosperms and broad-leaved angiosperms characteristic of warm climates. The POE is marked by a minor expansion of angiosperms and pteridophytes, warmer winters, and altered seasonal precipitation, followed by a return to pre-POE conditions. Increased terrestrial palynomorph concentrations before the CIE are suggestive of increased fluvial discharge before the PETM. Early PETM assemblages are characterized by dominance of ferns, loss of conifers, and expansion of broad-leaved angiosperm forests. Bioclimatic analyses indicate warmer mean atmospheric temperatures in early PETM time, driven primarily by winter warming of similar to 3 degrees C. A shift in seasonality, associated with increased severity of storms and floods that scoured the late Paleocene floodplain, facilitated establishment of riparian fern communities at the CIE onset. These flooding events persisted through the early part of the PETM and were severe enough to transport Westphalian-age (Middle Pennsylvanian) reworked material from the central Appalachian Basin and flush large amounts of terrestrial material and carbon onto the continental shelf, resulting in decreased salinity, increased productivity, and water-column stratification.

Ecological pressures on aquatic ecosystems have increased over recent centuries due to human activities and climate change. However, contextualizing ecosystem deterioration is often challenging due to limited knowledge of environmental changes over millennial timescales. Subtropical Carolina bays in North Carolina, USA, have remained unglaciated, preserving paleolimnological records that extend back to the last glacial period. Here, we analyzed a sediment core from the ecologically rich Lake Waccamaw spanning more than 28,000 years for aquatic proxies of nutrients, photosynthetic pigments, cyanotoxins, carbon isotopes, and terrestrial proxies of pollen and charcoal. The study explored paleolimnological changes in the aquatic environment connected to land changes and climate during the late Quaternary in the southeastern Atlantic Coastal Plain. Results reveal that while current levels of colonial cyanobacteria are high, past levels of cyanobacteria, other primary producers, and cyanotoxins were higher under natural climate variability. Abrupt ecosystem responses to increasing trophic conditions during Interstadial 3 (27.8-26.4 cal ka BP) and the early Holocene (11.4-7 cal ka BP) were marked by increases in primary producer abundance, deciduous vegetation expansion, and fire activity. Cyanobacteria remained dominant throughout the record, with colonial forms prevailing during the Holocene. Increases in pigment concentrations aligned with Quercus and were primarily driven by hydroclimatic variability and nutrient stoichiometry. Transitions between Pinus and Quercus pollen matched stadials and interstadials in the delta 18 Omicron record from the North Greenland Ice Core Project (NGRIP). This study highlights the value of multi-proxy millennial-scale paleolimnological records for understanding aquatic ecosystem responses to climate conditions during the late Pleistocene.

The Paleocene-Eocene Thermal Maximum (PETM, ~56 Mya) interval was marked by massive 13C-depleted carbon emissions into the ocean/atmosphere system, manifested as a negative carbon isotope excursion (CIE) in sedimentary components, and ~5 °C global average warming. Episodes of hydrological perturbations and soil-erosion have been widely documented for the PETM but their link with vegetation- and carbon cycle changes remain poorly constrained. Here, we present organic microfossil evidence showing a strong increase in fern-dominated pioneer vegetation that replaced coniferous forests on the margin of the Norwegian Sea during the first millennia of the CIE. With the present stratigraphic constraints, the "fern spike" occurred simultaneously in terrestrial settings along the North Sea, Arctic Ocean, the US east coast and in southern Australia, indicating that pioneer vegetation persisted for several millennia following a partial collapse of previously stable terrestrial ecosystems. Both the ferns and influx of microcharcoal imply recurrent physical disturbance, including soil destabilization and erosion, potentially linked to droughts, wildfires, and strong hydrological forcing resulting from extreme climate change. Together with evidence for reworked clay minerals and ancient organic matter (kerogen), these findings show that highly disturbed terrestrial ecosystems were widespread across mid- and high-latitude regions globally. Carbon cycle model simulations suggest that a substantial loss of standing and buried biomass, along with oxidation of soil organic matter, acted as important positive feedbacks during the onset of the CIE. Additionally, enhanced kerogen weathering likely contributed as another major positive feedback throughout both the onset and main phase of the CIE.

Over the past decades, many expanded sedimentary records from the US Atlantic Coastal Plain (ACP) have been studied in detail to assess causes and consequences of the Paleocene-Eocene Thermal Maximum (PETM; similar to 56 Ma). In ACP sections, the PETM, which is globally marked by a distinct negative carbon isotope excursion (CIE) lasting similar to 180 kyr following a large input of C-13-depleted carbon into the ocean-atmosphere system, has been recorded near the base of the Marlboro Clay. However, truly detailed site-to-site correlations within the CIE interval remain difficult in view of the absence of suitable stratigraphic markers offering the required resolution. Here, augmenting earlier studies involving various other marine microfossil groups, we present a high-resolution regional organic-walled dinoflagellate cyst (dinocyst) zonation scheme covering the uppermost Paleocene to lowermost Eocene sediments of the Aquia and Marlboro Clay formations at six ACP localities. We propose five latest Paleocene (ACP Pv-Pz) and six earliest Eocene (all within the PETM interval; ACP E0a-E0f) regional informal dinocyst zones. In addition, we emend the genus Hystrichokolpoma and employ several new species, of which four, viz. Impagidinium witmeri sp. nov., Nematosphaeropsis elongatus sp. nov., Hystrichokolpoma heroldiae sp. nov., and Cannosphaeropsis frielingii sp. nov., are formally described. Furthermore, we calibrate the dinocyst zones against magneto-, bio-, and ecostratigraphic records to allow robust regional correlation and age assessments with an average time resolution of < 10(5) years for the late Paleocene and < 104 years within the PETM interval. The scheme provides new opportunities for portraying the environmental and sedimentological evolution across the US Atlantic Coastal Plain during the PETM in unprecedented detail.

A previously buried paleosol was found on the continental shelf during a study of sea floor scour, nucleated by large artificial reef structures such as vessel hulks, barges, train cars, military vehicles, etc., called “scour nuclei”. It is a relic paleo-land surface of sapling-sized tree stumps, root systems, and fossil animal bone exhumed by scour processes active adjacent to the artificial reef structure. Over the span of five research cruises to the site in 2022–2024, soil samples were taken using hand excavation, PONAR grab samplers, split spoon, hollow tube auger, and a modified Shelby-style push box. High-definition (HD) video was taken using a Remotely Operated Vehicle (ROV) and diver-held cameras. Radiocarbon dating of wood samples returned ages of 42,015–43,417 calibrated years before present (cal yrBP). Pollen studies, together with the recovered macrobotanical remains, support our interpretation of the site as a freshwater forested wetland whose keystone tree species was Taxodium distichum—bald cypress. The paleosol was identified as an Aquult, a sub-order of Ultisols where water tables are at or near the surface year-round. A deep (0.25 m+) argillic horizon comprised the bulk of the preserved soil. Comparable Ultisols found in Georgia wetlands include Typic Paleaquult (Grady and Bayboro series) soils.

Peatland carbon storage is increasingly threatened by the combination of land-use change and climate variability, though carbon losses from land-use changes that span centuries are difficult to quantify, particularly in systems where little undisturbed area remains. Here we use a combination of vegetation change, fire history, and calculations of excess ash mass to quantify carbon loss in the Great Dismal Swamp National Wildlife Refuge (GDS NWR), USA, a highly impacted oligotrophic temperate peat swamp. Our results indicate that ditch construction that began in the Colonial Era in the late 1700s and continued into the mid-20th century across the swamp resulted in shifts from cypress-tupelo swamps to a combination of maple-gum and pine pocosin forests, consistent with drying surface conditions. Two large smoldering fires (2008, 2011) that were exacerbated by surface drainage, shifted vegetation from swamp to marsh, consumed peat over 25 km2, and caused losses of 1.05-1.34 Tg C due to peat burning. Across the Refuge as a whole, up to 48.2 Tg C has been lost to peat oxidation since ditch construction. Both stocks and rates of carbon loss remain higher than post-disturbance accumulation across most of GDS NWR, suggesting that existing efforts to block drainages to elevate water tables may not be enough to offset carbon losses. Rewetting heavily impacted surface peats may reduce peat oxidation and carbon loss, and shift vegetation toward hydrologic conditions preferred by pre-disturbance cypress-tupelo swamps.

Pollen and spores were recovered from the Paleocene Fort Union Formation and Paleocene–Eocene Willwood Formation of the Bighorn Basin (BHB), northwestern Wyoming, USA. In many local stratigraphic sections in the BHB, the base of the Eocene has been identified by the characteristic negative carbon isotope excursion (CIE) that marks the beginning of the Paleocene–Eocene Thermal Maximum (PETM). The palynotaxa from outcrop samples were examined using light microscopy (LM) and scanning electron microscopy (SEM). Seven new species are formally described (Tricolpites vegrandis, Rousea spatium, Striatricolporites astutus, Striatopollis calidarius, Friedrichipollis geminus, Retistephanocolporites modicrassus and Retistephanocolporites pergrandis). The temporal and geographic distributions of many of these palynotaxa suggest that hotter and more seasonally dry climates facilitated their northward range shifts during the PETM from the tropics or subtropics of the USA. For the temperate palynotaxa, the hotter and seasonally dry conditions resulted in local extirpation. A re-evaluation of the palynostratigraphic schemes established for the Paleocene–Eocene boundary confirms that the first appearance of Platycarya platycaryoides denotes the Paleocene–Eocene boundary in the Rocky Mountains region. A new Striatopollis calidarius Subzone, associated with early Wasatchian (Wa) Wa-0 and Wa-R faunas, is also recognized for CIE body localities in the BHB.

Wetlands cover a small portion of the world, but have disproportionate influence on global carbon (C) sequestration, carbon dioxide and methane emissions, and aquatic C fluxes. However, the underlying biogeochemical processes that affect wetland C pools and fluxes are complex and dynamic, making measurements of wetland C challenging. Over decades of research, many observational, experimental, and analytical approaches have been developed to understand and quantify pools and fluxes of wetland C. Sampling approaches range in their representation of wetland C from short to long timeframes and local to landscape spatial scales. This review summarizes common and cutting-edge methodological approaches for quantifying wetland C pools and fluxes. We first define each of the major C pools and fluxes and provide rationale for their importance to wetland C dynamics. For each approach, we clarify what component of wetland C is measured and its spatial and temporal representativeness and constraints. We describe practical considerations for each approach, such as where and when an approach is typically used, who can conduct the measurements (expertise, training requirements), and how approaches are conducted, including considerations on equipment complexity and costs. Finally, we review key covariates and ancillary measurements that enhance the interpretation of findings and facilitate model development. The protocols that we describe to measure soil, water, vegetation, and gases are also relevant for related disciplines such as ecology. Improved quality and consistency of data collection and reporting across studies will help reduce global uncertainties and develop management strategies to use wetlands as nature-based climate solutions.

During the Pleistocene, long-term trends in global climate were controlled by orbital cycles leading to high amplitude glacial-interglacial variability. The history of Amazonian vegetation during this period is largely unknown since no continuous record from the lowland basin extends significantly beyond the last glacial stage. Here we present a paleoenvironmental record spanning the last 1800 kyr based on palynological data, biome reconstructions, and biodiversity metrics from a marine sediment core that preserves a continuous archive of sediments from the Amazon River.Tropical rainforests dominated the Amazonian lowlands during the last 1800 ka interchanging with surrounding warm-temperate rainforests and tropical seasonal forests. Between 1800 and 1000 ka, rainforest biomes were present in the Amazon drainage basin, along with extensive riparian wetland vegetation. Tropical rainforest expansion occurred during the relatively warm Marine Isotope Stages 33 and 31 (ca. 1110 to 1060 ka), followed by a contraction of both forests and wetlands until ca. 800 ka. Between 800 and 400 ka, low pollen concentration and low diversity of palynological assemblages renders difficult the interpretation of Amazonian vegetation. A strong synchronicity between vegetation changes and glacial-interglacial global climate cycles was established around 400 ka. After 400 ka, interglacial vegetation was dominated by lowland tropical rainforest in association with warmer tem-peratures and higher CO2. During cooler temperatures and lower CO2 of glacial stages, tropical seasonal forests expanded, presumably towards eastern Amazonia. While this study provides no evidence sup-porting a significant expansion of savanna or steppe vegetation within the Amazonian lowlands during glacial periods, there were changes in the rainforest composition in some parts of the basin towards a higher proportion of deciduous elements, pointing to less humid conditions and/or greater seasonality of precipitation. Nevertheless, rainforest persisted during both glacial and interglacial periods. These findings confirm the sensitivity of tropical lowland vegetation to changes in CO2, temperature, and moisture availability and the most suitable conditions for tropical rainforests occurred during the warmest stages of the Mid Pleistocene Transition and during the interglacial stages of the past 400 kyr.(c) 2022 Elsevier Ltd. All rights reserved.

The Great Dismal Swamp wetland, spanning >400 km2 along the Virginia and North Carolina border, was shaped by a complex combination of geomorphic, climatic, and anthropogenic forcings during the last 14,000 years. Pollen, macrofossils, charcoal, and physical properties from sediment cores at seven sites provide a detailed record of the spatial heterogeneity of the wetland and the roles played by natural hydrologic variability, wildfire, and human modification of drainage in shaping vegetation and habitats. Cold-temperate forests occupied regional uplands from at least 13.5-10.3 cal ka BP. Marshes dominated by grasses and other herbaceous taxa began developing along low-elevation streams as early as 10.3 cal ka BP, resulting in accumulation of organic silts. Long-hydroperiod, peat accumulating marshes, with abundant floating aquatic plants, developed as early as 9.6 cal ka BP, as rapid rates of sea-level rise elevated the water table and facilitated wetland development and peat accumulation along stream courses. By the mid-Holocene (c. 7-6.5 cal ka BP), when local sea-level rise began slowing and reached about 12-15 m below present, shorter hydroperiod, peat-accumulating marshes dominated the land-scape, with increased wildfire activity. Great Dismal Swamp vegetation shifted from marshes to peat -accumulating forested wetlands by c. 3.7 cal ka BP; these were dominated by varying combinations of Nyssa (tupelo), Taxodium (cypress), and Chamaecyparis thyoides (Atlantic white cedar). Wildfires were infrequent during this time, and the forested wetlands persisted, with minor compositional changes related to climate-driven fluctuations in stream flow, until colonial ditching and logging began in the swamp during the late 18th century. These activities decreased cypress and cedar populations, and, by the mid-20th century, expanded ditching resulted in even drier conditions and expansion of maple-gum (dominated by Acer and Liquidambar), and pine-pocosin (dominated by Pinus) forests. The distribution of these forests differs from that of the late Holocene and represents a fundamental shift in hydrology, peat structure, vegetation, and fire regime due to landscape alterations of the last few centuries. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http:// creativecommons.org/licenses/by-nc-nd/4.0/).

This paper broadens the description of sedimentary organic matter from the conventional use of coal petrography to include palynological and geochemical sedimentary organic matter. Palynological sedimentary organic matter includes all chemically resistant organic microfossils, such as pollen and spores, dinocysts, microforaminifera (chitinoid-like linings of foraminifera), microscopic algae, charcoal, palynodebris, acritarchs, chitinozoans, and scolecodonts. Geochemical sedimentary organic matter includes organic biomarkers, lipids, and photosynthetic pigments. We provide examples of the use of palynological and geochemical analysis of sedimentary organic matter to understand patterns and impacts of changing climate, fire regimes, hydrologic extremes, water quality, and land change on terrestrial and marine systems through geologic time to provide a long-term perspective to evaluate anthropogenic impacts on the Earth as well as to support forensics investigations.

Paleoclimate reconstructions can provide a window into the environmental conditions in Earth history when atmospheric carbon dioxide concentrations were higher than today. In the eastern USA, paleoclimate reconstructions are sparse, because terrestrial sedimentary deposits are rare. Despite this, the eastern USA has the largest population and population density in North America, and understanding the effects of current and future climate change is of vital importance. Here, we provide terrestrial paleoclimate reconstructions of the eastern USA from Miocene fossil floras. Additionally, we compare proxy paleoclimate reconstructions from the warmest period in the Miocene, the Miocene Climatic Optimum (MCO), to those of an MCO Earth System Model. Reconstructed Miocene temperatures and precipitation north of 35°N are higher than modern. In contrast, south of 35°N, temperatures and precipitation are similar to today, suggesting a poleward amplification effect in eastern North America. Reconstructed Miocene rainfall seasonality was predominantly higher than modern, regardless of latitude, indicating greater variability in intra-annual moisture transport. Reconstructed climates are almost uniformly in the temperate seasonal forest biome, but heterogeneity of specific forest types is evident. Reconstructed Miocene terrestrial temperatures from the eastern USA are lower than modeled temperatures and coeval Atlantic sea surface temperatures. However, reconstructed rainfall is consistent with modeled rainfall. Our results show that during the Miocene, climate was most different from modern in the northeastern states, and may suggest a drastic reduction in the meridional temperature gradient along the North American east coast compared to today.