Visceral inflammation evokes hyperexcitability in nociceptive dorsal root ganglia (DRG) neurons and these changes are associated with increased voltage-gated sodium channel (Na(v)) 1.8 current density, but the molecular determinants of these changes are unclear. This study used Western blotting to measure changes in Na(v) 1.7, 1.8 and 1.9 protein expression during trinitrobenzenesulphonic acid (TNBS) colitis and quantitative polymerase chain reaction (PCR) to examine corresponding changes in mRNA. Colonic neurons were labelled with the retrograde tracer Fast Blue injected into the wall of the distal colon and quantitative PCR performed on laser-captured labelled colonic neurons from ganglia at T9-13 or unlabelled DRG neurons from the upper spinal cord. Immunohistochemistry and western blots were performed on whole DRG from the same sites. Fast Blue-labelled neurons demonstrated Na(v) 1.7, 1.8 and 1.9 immunoreactivity. On day 7 of colitis, which correlated with electrophysiological studies, there was a threefold increase in Na(v) 1.8 protein in ganglia from T9 to 13, but Na(v) 1.7 and 1.9 levels were unchanged. There was no corresponding change in the Na(v) 1.8 alpha-subunit mRNA levels. However, on days 2 and 4, Na(v) 1.8 mRNA was decreased 10-fold. Na(v) 1.8 protein and mRNA levels were unchanged in neurons isolated from ganglia in the upper spinal cord, where colonic neurons are not found. These findings suggest that the TNBS evoked increase in Na(v) 1.8 currents is associated with increased numbers of channels. The absence of corresponding changes in transcript suggests a translational or post-translational mechanism, but the 10-fold recovery of transcript preceding this time point also demonstrates a complex transcriptional regulation.

Recently, the research and development activities for replacing Pb-containing solders with Pb-free solders have been intensified due to both competitive market pressures and environmental issues. As a result of these activities, a few promising candidate solder alloys have been identified, mainly, Sn-based alloys. A key issue affecting the integrity and reliability of solder joints is the interfacial reactions between a molten solder and surface finishes in the solder joint structures. In this paper, a fundamental study of the interfacial reactions between several Pb-free candidate solders and surface finishes commonly used in printed-circuit cards is reported. The Pb-free solders investigated include Sn-3.5Ag, Sn-3.8Ag-0.7Cu, and Sn-3.5Ag-3.0Bi. The surface finishes investigated include Cu, Au/Ni(P), Au/Pd/Ni(P), and Au/Ni (electroplated). The reaction kinetics of the dissolution of surface finishes and intermetallic compound growth have been measured as a function of reflow temperature and time. The intermetallic compounds formed during reflow reactions have been identified by SEM with Energy Dispersive X-ray Spectroscopy.

Background and Objectives: Hyperconcentration of platelets may lead to platelet activation and loss of platelet function. Materials and Methods: Platelet activation following hyperconcentration was assessed using flow-cytometric detection of platelet P-selectin expression and platelet swirling. Results: Platelet hyperconcentration led to a minimal increase in P-selectin expression and no difference in platelet swirling. Conclusion: Hyperconcentration was not associated with a clinically significant change in platelet activation and had no significant effect on platelet quality as detected by pH and platelet yield.

BACKGROUND AND OBJECTIVES Hyperconcentration of platelets may lead to platelet activation and loss of platelet function. MATERIALS AND METHODS Platelet activation following hyperconcentration was assessed using flow-cytometric detection of platelet P-selectin expression and platelet swirling. RESULTS Platelet hyperconcentration led to a minimal increase in P-selectin expression and no difference in platelet swirling. CONCLUSION Hyperconcentration was not associated with a clinically significant change in platelet activation and had no significant effect on platelet quality as detected by pH and platelet yield.

OBJECTIVE CD4 and CD8 antigen coexpression occurs not only on blastic T-cell malignancies, but also on a small subset of mature lymphocytes. The aim of this study was to determine the prevalence of this population of cells and to identify features that can be used to differentiate them from T lymphoblasts. DESIGN All specimens submitted to the clinical flow cytometry laboratory from August 1, 1994, through July 31, 1995, were analyzed for CD4 and CD8 coexpression. MAIN OUTCOME MEASURE Percentage of lymphocytes coexpressing the CD4 and CD8 antigens. RESULTS Four percent (22/526) of all specimens contained a population of CD4/CD8 coexpressing cells. Five cases represented CD4 and CD8 antigen expression on neoplastic cells. In 17 cases, the CD4/CD8 coexpressing cells appeared to represent a population of mature lymphocytes with a normal phenotype. The immature cells of T-cell acute lymphocytic leukemia and lymphoblastic lymphoma represented a dominant uniform population of cells demonstrating strong staining with both the CD4 and CD8 antigens. Cases containing a mature population of CD4/CD8 coexpressing cells were characterized by fewer coexpressing cells and variable expression of CD8. There were cases where distinction of this population of mature CD4/CD8 coexpressing lymphoid cells from a blastic malignancy was not possible using immunophenotyping alone. CONCLUSION Correlation of clinical, morphologic, and immunophenotypic data is recommended to prevent the misdiagnosis of subtle involvement by a blastic T-cell malignancy.

Antibodies directed against CD20 (L26, Leu 16, and B1) are frequently used to determine the presence of B lymphocytes. However, recent publications describe the unexpected presence of CD20-positive T cells in the peripheral blood of normal subjects and occasional T-cell neoplasms that express CD20. To determine the presence of CD20-positive T cells in bone marrow, flow cytometric analysis was performed on 34 aspirate specimens (14 normal, 5 acute lymphoblastic lymphoma [ALL], 5 acute myelogenous leukemia [AML], 4 HIV positive, 2 myelodysplastic/myeloproliferative, 2 chronic myelogenous leukemia [CML], 1 chronic lymphocytic lymphoma [CLL], 1 multiple myeloma). A small population of cells coexpressing CD3 (Leu 4) and CD20dim (Leu 16) was identified in 94% of the specimens, representing 0% to 11% (mean 1.77%) of marrow mononuclear cells and 0% to 22.2% (mean 6.54%) of marrow lymphoid cells. There was no correlation between the percentage of CD20-positive T cells and the CD4:CD8 ratio, patient age, gender, or diagnosis. CD20dim positive cells included immature B cells and CD20-positive T cells. Although evaluation of CD20 expression is useful in delineating B-cell processes, caution should be exercised in interpreting its expression on bone marrow T-lymphoid cells. CD20 expression on T cells may be seen in either normal, reactive, or neoplastic processes.

Crop ScienceVolume 29, Issue 5 cropsci1989.0011183X002900050053x p. 1328-1328 Registration of Crop Cultivars Registration of ‘Fiesta II’ Perennial Ryegrass G.W. Pepin, Corresponding Author G.W. Pepin n/[email protected] Pickseed West, Inc., P.O. Box 888, Tangent, OR, 97389Corresponding author.Search for more papers by this authorW.K. Wiley, W.K. Wiley Pickseed West, Inc., P.O. Box 888, Tangent, OR, 97389Search for more papers by this authorD.E. King, D.E. King Pickseed West, Inc., P.O. Box 888, Tangent, OR, 97389Search for more papers by this authorC.J. Petersen, C.J. Petersen 1997 Skyline Drive, Orem, UT, 84057Search for more papers by this authorC.R. Funk, C.R. Funk Soils and Crops Dep., New Jersey Agric. Exp. Stn., Cook College, Rutgers Univ., New Brunswick, NJ, 08903Search for more papers by this author G.W. Pepin, Corresponding Author G.W. Pepin n/[email protected] Pickseed West, Inc., P.O. Box 888, Tangent, OR, 97389Corresponding author.Search for more papers by this authorW.K. Wiley, W.K. Wiley Pickseed West, Inc., P.O. Box 888, Tangent, OR, 97389Search for more papers by this authorD.E. King, D.E. King Pickseed West, Inc., P.O. Box 888, Tangent, OR, 97389Search for more papers by this authorC.J. Petersen, C.J. Petersen 1997 Skyline Drive, Orem, UT, 84057Search for more papers by this authorC.R. Funk, C.R. Funk Soils and Crops Dep., New Jersey Agric. Exp. Stn., Cook College, Rutgers Univ., New Brunswick, NJ, 08903Search for more papers by this author First published: 01 September 1989 https://doi.org/10.2135/cropsci1989.0011183X002900050053xCitations: 2AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat No abstract is available for this article.Citing Literature Volume29, Issue5September–October 1989Pages 1328-1328 RelatedInformation

Crop ScienceVolume 28, Issue 6 cropsci1988.0011183X002800060036x p. 1020-1021 Registration of Crop Cultivars Registration of ‘Victory’ Chewings Fescue G. W. Pepin, G. W. Pepin Pickseed West Inc., P.O. Box 888, Tangent, OR, 97389Search for more papers by this authorW. K. Wiley, W. K. Wiley Pickseed West Inc., P.O. Box 888, Tangent, OR, 97389Search for more papers by this authorD. E. King, D. E. King Pickseed West Inc., P.O. Box 888, Tangent, OR, 97389Search for more papers by this authorB. B. Clarke, B. B. Clarke Pickseed West Inc., P.O. Box 888, Tangent, OR, 97389Search for more papers by this authorC. R. Funk, Corresponding Author C. R. Funk n/a@dne.dne Plant Pathology Dep., Soils and Crops Dep., New Jersey Agric. Exp. Stn., Cook College, Rutgers Univ., New Brunswick, NJ, 08903Corresponding author.Search for more papers by this author G. W. Pepin, G. W. Pepin Pickseed West Inc., P.O. Box 888, Tangent, OR, 97389Search for more papers by this authorW. K. Wiley, W. K. Wiley Pickseed West Inc., P.O. Box 888, Tangent, OR, 97389Search for more papers by this authorD. E. King, D. E. King Pickseed West Inc., P.O. Box 888, Tangent, OR, 97389Search for more papers by this authorB. B. Clarke, B. B. Clarke Pickseed West Inc., P.O. Box 888, Tangent, OR, 97389Search for more papers by this authorC. R. Funk, Corresponding Author C. R. Funk n/a@dne.dne Plant Pathology Dep., Soils and Crops Dep., New Jersey Agric. Exp. Stn., Cook College, Rutgers Univ., New Brunswick, NJ, 08903Corresponding author.Search for more papers by this author First published: 01 November 1988 https://doi.org/10.2135/cropsci1988.0011183X002800060036xAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat No abstract is available for this article. Volume28, Issue6November–December 1988Pages 1020-1021 RelatedInformation

Crop ScienceVolume 28, Issue 6 cropsci1988.0011183X002800060035x p. 1020-1020 Registration of Crop Cultivars Registration of ‘Spartan’ Hard Fescue G. W. Pepin, G. W. Pepin Pickseed West Inc., P.O. Box 888, Tangent, OR, 97389Search for more papers by this authorW. K. Wiley, W. K. Wiley Pickseed West Inc., P.O. Box 888, Tangent, OR, 97389Search for more papers by this authorD. E. King, D. E. King Pickseed West Inc., P.O. Box 888, Tangent, OR, 97389Search for more papers by this authorR. W. Duell, R. W. Duell Pickseed West Inc., P.O. Box 888, Tangent, OR, 97389Search for more papers by this authorC. R. Funk, Corresponding Author C. R. Funk n/a@dne.dne Soils and Crops Dep., New Jersey Agric. Exp. Stn., Cook College, Rutgers Univ.,, New Brunswick, NJ, 08903Corresponding author.Search for more papers by this author G. W. Pepin, G. W. Pepin Pickseed West Inc., P.O. Box 888, Tangent, OR, 97389Search for more papers by this authorW. K. Wiley, W. K. Wiley Pickseed West Inc., P.O. Box 888, Tangent, OR, 97389Search for more papers by this authorD. E. King, D. E. King Pickseed West Inc., P.O. Box 888, Tangent, OR, 97389Search for more papers by this authorR. W. Duell, R. W. Duell Pickseed West Inc., P.O. Box 888, Tangent, OR, 97389Search for more papers by this authorC. R. Funk, Corresponding Author C. R. Funk n/a@dne.dne Soils and Crops Dep., New Jersey Agric. Exp. Stn., Cook College, Rutgers Univ.,, New Brunswick, NJ, 08903Corresponding author.Search for more papers by this author First published: 01 November 1988 https://doi.org/10.2135/cropsci1988.0011183X002800060035xAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat No abstract is available for this article. Volume28, Issue6November–December 1988Pages 1020-1020 RelatedInformation

Crop ScienceVolume 24, Issue 6 cropsci1984.0011183X002400060053x p. 1211-1212 Registration of Crop Cultivars Registration of Mustang Tall Fescue C. R. Funk, C. R. FunkSearch for more papers by this authorW. Kent Wiley, W. Kent WileySearch for more papers by this authorDouglas E. King, Douglas E. KingSearch for more papers by this authorMichael F. Robinson, Michael F. RobinsonSearch for more papers by this author C. R. Funk, C. R. FunkSearch for more papers by this authorW. Kent Wiley, W. Kent WileySearch for more papers by this authorDouglas E. King, Douglas E. KingSearch for more papers by this authorMichael F. Robinson, Michael F. RobinsonSearch for more papers by this author First published: 01 November 1984 https://doi.org/10.2135/cropsci1984.0011183X002400060053xCitations: 2AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat No abstract is available for this article.Citing Literature Volume24, Issue6November–December 1984Pages 1211-1212 RelatedInformation

Crop ScienceVolume 22, Issue 1 cropsci1982.0011183X002200010043x p. 159-159 Registration of Crop Cultivar Registration of Dasher Perennial Ryegrass1 (Reg. No. 75) C. R. Funk, C. R. FunkSearch for more papers by this authorR. E. Engel, R. E. EngelSearch for more papers by this authorM. C. Pick, M. C. PickSearch for more papers by this authorW. K. Wiley, W. K. WileySearch for more papers by this authorD. E. King, D. E. King Research professor and professor, respectively, Soils and Crops Dep., New Jersey Agric. Exp. Stn.; director, president and senior agronomist, respectively, Pickseed West, Inc., P.O. Box 888, Tangent, OR 97389.Search for more papers by this author C. R. Funk, C. R. FunkSearch for more papers by this authorR. E. Engel, R. E. EngelSearch for more papers by this authorM. C. Pick, M. C. PickSearch for more papers by this authorW. K. Wiley, W. K. WileySearch for more papers by this authorD. E. King, D. E. King Research professor and professor, respectively, Soils and Crops Dep., New Jersey Agric. Exp. Stn.; director, president and senior agronomist, respectively, Pickseed West, Inc., P.O. Box 888, Tangent, OR 97389.Search for more papers by this author First published: 01 January 1982 https://doi.org/10.2135/cropsci1982.0011183X002200010043x 1 Registered by the Crop Sci. Soc. Am. Paper of the Journal Series, New Jersey Agric. Exp. Stn., Cook College, Rutgers Univ., New Brunswick, NJ 08903. AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat No abstract is available for this article. Volume22, Issue1January–February 1982Pages 159-159 RelatedInformation

The effect of sizing mixtures on the slubby portions of a staple yarn was determined indirectly by the application of the size to a cotton roving and subsequent testing of the roving for strength, ultimate elongation, and stiffness. In contrast to the methods which employ yarn as the base material for the application of the size, large differences between sizing mixtures were found by this procedure. The evaluation method which was investigated proved useful for the screening of sizes for knitting yarns in which the failure to give good knitting quality was caused by the presence of slubs.