The treatment of the dead body is a highly social phenomenon, rooted in cultural conventions. Choices in mortuary rites include inhumation, cremation, and excarnation, along with secondary manipulation before or after burial. In the Carpathian Basin between 1600 and 700 b.c., people employed diverse choices in peri- and post-mortem treatment of human remains. Beginning by defining local cremation conventions, including fragmentation of the body, we next present new data to reconstruct excarnation and inhumation practices. These display similar patterns of peri- and post-mortem manipulation in both cemetery and settlement contexts, demonstrating intersections between rites. Engagement with human bones in Late Bronze and Early Iron Age contexts is shown to have been ubiquitous and encompass varying states of integrity, revealing distinct practices surrounding their use or reuse. This shows that human remains, as material culture, played myriad roles in social discourses, ranging from place-making to ancestor reverence to domination of others.

The present study aims to explore connectivity and networking in Late Bronze Age (LBA)/Early Iron Age (EIA) Greece and the Balkans using morphological biodistance analysis and test the potentiality of newly introduced statistical tests, which were designed for challenging datasets, in this particular cultural area. Cranial non-metric traits were recorded in ten skeletal collections, spanning from East Crete to Romania. We followed an experimental statistical approach encompassing two different measures of divergence, the conventional and well-tested mean measure of divergence (MMD) and the newly introduced untransformed measure of divergence (UMD). Though different, results based on these two measures are mutually supporting and show that biodistances in our regional case studies mainly follow the isolation by distance model. This cautiously confirms our main hypothesis that during the LBA and EIA periods in Greece and the Balkans, personal mobility was a slow process characterized by integration, rather than displacement or transformation. The current study is the first one to infer biological affinities using cranial non-metric analysis combined with artifactual evidence, in LBA/EIA Greece and the Balkans. Building a larger dataset through future non-metric analyses will better enable exploring networking and mobility to further complement ongoing bioarchaeological, genetic, and material culture studies.

Objectives: Isotopic analysis has been proven a valuable tool for reconstructing dietary habits in the ancient world. In this work we examine the evolution of Greek diet from the Neolithic period (6800-3000 BC) to modern times. Materials and Methods: We present isotopic analysis results for delta C-13 and delta N-15 of adult human bone collagen from ancient (Kaiadas in Sparta) and modern populations (Crete), while at the same time we review published results for delta C-13 and delta N-15 of adult human bone collagen from Neolithic to modern times of Greece. Results: The delta C-13 isotopic values evolve to more positive values, while they present a greater variability toward modern times. Furthermore, the delta N-15 values gradually increase over time. Discussion: We discuss the differences i) in human diet through time for the whole Greece and ii) individually for the island of Crete and we conclude that over time human diet becomes richer in high trophic level proteins such as marine sources with a larger variety of food sources.

In this article we present an isotopic analysis of human bone collagen (δ13Ccol, and δ15Ncol) and bone apatite (δ13C) for diet reconstruction, as well as δ18Oap of human bone apatite for climate reconstruction, using samples from Northern Greece. Radiocarbon dating analysis was conducted on three of the Agras samples and the results (from 1000 to 800 BC) correspond to the Early Iron Age. Isotopic values for δ13Ccol range from −20.5‰ to −16‰ and for δ15Ncol from 6‰ to 11.1‰—a strong indication of a C3-based diet, with contributions by C4 and freshwater fish elements. The results were compared to the ones from Roman Edessa, and Alexandreia (a contemporary city near Edessa), as well as to other Early Iron sites in Greece and wider Europe. In general, the results from Agras are in good agreement with the results from Northern Greece, with the exception of the Makriyalos site, and are quite close to those of Croatia’s and Hungary’s sites. Additionally, from the δ18Oap results we calculated the oxygen isotopic composition of consumed water for Agras (from −9.6‰ to −10.9‰) and for Roman Edessa (from −9.6‰ to −11.2‰) for the palaeoclimate and palaeomobility reconstruction.

This paper investigates the efficacy of comparative bioarchaeological approaches in exploring the impact of economic marginality on human lifeways. Skeletal remains from the Late Bronze Age cemetery of Achlada in Northern Greece were chosen to address this, as this specific community was probably less well networked, evident in its location away from major communication routes and the paucity of grave goods at the site. A biocultural methodology combining comparative data on funerary practices and lifestyle was implemented. Sex differences were found within the community and seem to agree with the differential burial placement of the sexes possibly representing the different roles that society symbolically attributed to men and women in deathways. Comparative intercemetery data did not reveal poorer health and diet, or more intense physical activity, compared to well-networked sites. Nonetheless, Achlada, as well as numerous, mostly north communities of the wider context, probably faced more physiological challenges during growth, at least of a mild to moderate level, compared to a number of populations connected by major communication routes. The current study highlights the importance of implementing comparative bioarchaeological approaches as a means of identifying the impact of marginality on human lifeways, particularly in settings with limited material culture information.Limitations linked to preservation issues and the multifactorial nature of lifestyle indicators could be dealt with by future biomolecular and isotopic analyses. Η παρούσα εργασία έχει στόχο να διερευνήσει το κατά πόσο οι συγκριτικές βιοαρχαιολογικές προσεγγίσεις είναι εφικτό να δώσουν απαντήσεις ως προς την επίδραση της οικονομικής περιθωριοποίησης στον ανθρώπινο τρόπο διαβίωσης. Επιλέχθηκαν σκελετικά κατάλοιπα της Ύστερης Εποχής Χαλκού από το νεκροταφείο της Αχλάδας στη Βόρεια Ελλάδα ώστε να απαντηθεί το εν λόγω ερώτημα, καθώς η συγκεκριμένη κοινωνία -βάσει της θέσης της μακριά από τα μεγάλα δίκτυα επικοινωνίας και της παρουσίας πολύ λίγων ταφικών ευρημάτων-ήταν πιθανώς λιγότερο καλά δικτυωμένη. Ακολουθήθηκε μια συνδυαστική βιοπολιτισμική προσέγγιση συγκριτικών ταφικών δεδομένων και συγκριτικών αποτελεσμάτων δεικτών τρόπου διαβίωσης. Βρέθηκαν διαφορές μεταξύ των δύο φύλων στον εν λόγω πληθυσμό οι οποίες φαίνεται να συμφωνούν με την διαφορετική πλευρά κατάκλισής τους, η οποία πιθανώς να συμβόλιζε τη διαφορετικότητα των ρόλων που η κοινωνία απέδιδε σε άντρες και γυναίκες στο ταφικό περιβάλλον. Συγκριτικά αποτελέσματα μεταξύ νεκροταφείων δεν φανέρωσαν χαμηλότερο επίπεδο υγείας και διατροφής, ούτε πιο έντονη εργασιακή καταπόνηση, σε σχέση με καλά δικτυωμένες θέσεις. Παρόλα αυτά, η Αχλάδα, όπως και μια σειρά –κυρίως βόρειων- κοινωνιών τουευρύτερου πλαισίου, πιθανότητα αντιμετώπισαν περισσότερα φαινόμενα καταπόνησης (στρες) κατά τη διάρκεια της ανάπτυξης, τουλάχιστον ήπιου και μετρίου επιπέδου, συγκριτικά με καλύτερα δικτυωμένους πληθυσμούς. Η παρούσα εργασία τονίζει τη σημασία της εφαρμογής συγκριτικών βιοαρχαιολογικών προσεγγίσεων ως μέσο μελέτης της επίδρασης της περιθωριοποίησης στους ανθρώπινους πληθυσμούς, ιδιαιτέρως σε θέσεις με περιορισμένες πληροφορίες υλικού πολιτισμού. Μεθοδολογικοί περιορισμοί οι οποίοι συνδέονται με ζητήματα διατήρησης αλλά και με τον πολυπαραγοντικό χαρακτήρα των δεικτών τρόπου διαβίωσης, ενδεχομένως να αντιμετωπιστούν μέσω των επερχόμενων βιομοριακών και ισοτοπικών αναλύσεων.

We review the stable isotopic data of recovered Greek bones from the Early Neolithic to the Late Bronze period in order to examine dietary changes over time. As an isotopic baseline we use the published fauna data of the periods. The analysis revealed a diet that included a significant proportion of foods based on C3 plants, and the bulk of the animal protein must have been provided by terrestrial mammals with a small but detectable proportion of marine protein for coastal and island populations. A more significant contribution of marine protein is observed for Bronze Age populations while the enrichment in both C and N isotopes is connected, for some areas, to the introduction of millet during the Bronze Age, and to freshwater consumption. An extensive database of Greek food sources is presented and compared to the fauna from the prehistoric periods (Early Neolithic to Late Bronze Age) of the literature. We propose that this database can be used in palaeodiet reconstruction studies.

The present isotopic study is the first to assess the dietary habits of a Roman population from Northern Greece. A multi-stable isotope analysis was conducted in 22 individuals deriving from Edessa, a town in Greek Macedonia dated at 2nd-4th c. CE. The basic aims of the present study are to reconstruct the diet of this population, to explore possible dietary variations in relation to sex and age and to detect possible differentiations between the adult and the childhood/juvenile diet. The results of this study revealed that residents of Edessa probably consumed C-3 and C-4 items as their primary protein source. In addition, the collagen results along with the collagen-apatite spacing possibly indicated a detectable consumption of freshwater sources, for three singular cases. There were no significant isotopic differences between males and females, or between younger and middle aged adults, whereas indications of a possible differentiation among the juvenile and the adult diet were noted. This study offers new information regarding an underrepresented period in Greek history and hopes to encourage the inclusion of multiple isotopic proxies for dietary reconstruction of ancient populations in Greece.

In the present isotopic study we assess the dietary patterns of a medieval population in Greece. Three isotopes were measured (δ13Ccoll, δ13Cap, δ15Ν) in a skeletal sample from Thebes, consisting of 16 adult individuals (7 males, 9 females) dated at 13th–14th c. CE. According to historical sources many different ethnicities co-existed in Thebes during the stated period, as Greece was under the Crusader's occupation. Thus, a possible dietary diversity could be expected. The basic aims of the present study are to reconstruct the dietary habits of the stated population by quantifying the individual human diet, to detect possible dietary differentiations in relation to sex and age and to compare our results with the ones of other medieval sites primarily in Greece and secondly in the Mediterranean. According to the isotopic and statistical analysis the population studied showed a significant diversity, as half of the sample assessed seemed to have been reliant on a typical C3 terrestrial diet, whereas the other half consumed both C3 and C4 sources. In particular, C4 items (i.e. millet) seem to have been a major staple for a singular female adult (Th22; δ13Ccoll: −11‰; δ15N: 10‰; δ13Cap: −5.5‰). In addition, based on the Δ13Cap-coll values, it seems possible that aquatic sources constituted a food source for three specific adults. Therefore, this study sheds light to the importance of identifying singular cases in generic isotopic patterns, and encourages the inclusion of apatite's signature in palaeodietary reconstructions of populations from Greece.

Even though many isotopic studies have been conducted on ancient populations from Greece for the purpose of dietary reconstruction; mostly through carbon and nitrogen isotopic signals of bone collagen, less attention has been given to the utility of apatite signatures (oxygen and carbon) as dietary and palaeoenvironmental tools. Moreover, until recently the isotopic signal of tooth enamel for both the purposes of environmental and dietary reconstructions has been rarely assessed in ancient Greek societies. Therefore, the present study aims to provide with novel isotopic information regarding Edessa; a town in Northern Greece, during the Roman period. The current study primarily aims to explore the possible differentiation between the present climatic conditions in Edessa in relation to those occurring at the Roman period. Secondly, this study aims to reveal the significant utility of enamel isotopic signatures (carbon and oxygen) in palaeoenvironmental and palaeodietary studies regarding ancient human remains. The isotopic analyses have been conducted at the Stable Isotope and Radiocarbon Unit of INN, NCSR "Demokritos". The population of Roman Edessa (2nd-4th c. AD) consists of 22 individuals, providing with 19 bone samples and 16 enamel ones. The mean enamel oxygen value is at -7.7 +/- 1.1 %, the bone apatite mean oxygen value at -9.2 +/- 1.9 %, and finally the mean carbon enamel value is at -11.7 +/- 1.2 %. Oxygen values probably indicate that Edessa had a cooler climate during the Roman times in relation to present conditions, even though more research should be carried out in order to be more certain. In addition, the possible existence of non-local individuals has been revealed through the oxygen teeth enamel-bone apatite spacing. Finally, the carbon enamel signature has pointed out possible differentiations between the adult and the juvenile diet. Based on Edessa's findings, the stated study strongly encourages the enamel oxygen and carbon isotopic signals as palaeoclimatological and palaeodietary tools respectively.

EJI-PATER 1st Young Researchers’ Meeting in Heritage and Territory, Braga, Portugal, 14-16 2017

Assessing the subsistence strategies of past populations; through their dietary and occupational patterns; could provide important information regarding social status and possible gender differences, especially in turbulent historical periods, as the one of the Crusader’s occupation in Greece (1204-1460 AD). Therefore, the human sample from Aghia Triada in Thebes (13th-14th c. AD) serves as the ideal skeletal material. Diet was explored through two dental indicators; dental caries and tooth wear, while occupational stress was explored through three activity markers; osteoarthritis (OA), spinal facet remodeling and Schmorl’s nodes. The aims of the present study are to assess the dietary and activity patterns of the stated population and explore possible sex differentiations. A total of 126 teeth and 350 vertebrae have been examined. The entire population presents a caries rate of 16.7%, and males present a much higher caries frequency than females (25.5% males vs. 9.9% females). Furthermore, females present significantly higher rates of osteophytes than males, whereas no significant sex differences were found regarding facet remodeling and Schmorl’s nodes. Dental results confirm historical information of medieval Thebes having an agricultural economy and are also in agreement with isotopic data. In addition, our findings suggest very intense physical activity for both sexes, whereas the distribution of facet remodeling along the spine could indicate a possible gender division of labor. Our study proposes two positive correlations; between facet remodeling and osteophytes, and between Schmorl’s nodes and facet remodelling; as activity indicators in past or/and modern populations. Finally, we strongly encourage the inclusion of spinal facet remodelling in studies focusing on occupational stress.

Even though isotopic analyses have been extensively implemented on human skeletal remains for the purpose of dietary reconstruction, less attention has been given to the ingested water and thus to the investigation of palaeoclimatic conditions. In particular, oxygen isotopic fingerprinting has never been applied on human skeletal remains from Greece for the abovementioned purpose before. The basic aim of the present study is to compare climatic conditions from two ancient populations, deriving from two different ecological locations; Edessa (Greek Macedonia; 2nd-4th c. AD) and Thebes (Sterea Hellas, 13th-14th c. AD). Oxygen values in Edessa are at -7.69 ±1.13 ‰ and -9.18 ±1.88 ‰ for tooth enamel and bone apatite respectively. On the other hand, oxygen signals in Thebes are at -5.8 ±2.16 ‰ and -9.23 ±1.3 % for the enamel and bone apatite respectively. The utility of oxygen isotopic signatures for the purpose of palaeoclimatic investigation lies on the fact that the ratio of 18 to 16O of meteoric precipitation, expressed as δ18O per mill (‰), relative to the international standard (vSMOW) varies geographically by temperature, humidity, evaporation, distance to the sea, altitude and latitude. Therefore, results as expected, point out that Edessa do presents more negative enamel isotopic values in relation to Thebes, however the noted difference is not observed for the bone apatite samples. The lack of bone apatite differentiation between sites could be attributed to cultural diversity (particularly in Thebes), shift in dietary habits due to migration or social status, climatic fluctuations within each site or to possible diagenetic alteration of bone apatite samples.