Manganese (Mn) plays an important role in marine biogeochemical cycling; however, the mechanisms of microbial Mn oxidation in deep-sea environments remain poorly understood. This study investigated the Mn tolerance and precipitation capabilities of a novel bacterial isolate, Staphylococcus ureilyticus GOM10, isolated from deep-sea water in the Gulf of Mexico. Using a multidisciplinary approach combining microbiological, microscopic, spectroscopic, genomic, and geochemical modeling techniques, we elucidated the genetic pathways underlying Mn oxidation in GOM10. Scanning electron microscopy and energy-dispersive X-ray spectroscopy confirmed the presence of Mn-bearing precipitates associated with the bacterial cells. The strain demonstrated tolerance to Mn(II) concentrations up to 150 mM and could oxidize Mn(II) at a rate of 5.2-29.2 nM h(-1). Genomic analysis revealed genes related to Mn transport and oxidation, including superoxide dismutase (SOD), peroxidases, and Mn-dependent regulators, suggesting that the Mn oxidation mechanism involves the generation of reactive oxygen species (ROS). The proposed oxidation pathway couples Mn(II) oxidation with cellular protection against oxidative stress. Our findings highlight the importance of investigating alternative pathways, such as ROS-mediated oxidation, for a more comprehensive understanding of Mn biomineralization in deep-sea environments. This study provides insights into microbial adaptations in deep-sea environments and the potential role of heterotrophic bacteria in Mn cycling.

Spanning from the North American continent to the Hawaiian Ridge and from the Aleutian Ridge to the tip of the Baja California Peninsula, the deep Northeast Pacific (>200 m depth) hosts a high diversity of species and habitats. While relatively well studied in comparison to other regions, deep-sea ecosystems of the Northeast Pacific still hold many unknowns. This review provides an overview of the current state of knowledge of deep pelagic and benthic ecosystems in the Northeast Pacific in the context of climate change and increasing anthropogenic pressures. Regional trends in biodiversity, area-based management measures and research effort are synthesized to identify gaps in scientific knowledge, management and conservation. Based on the compilation of available literature and data extracted from open access databases, we found that scientific knowledge and research effort were unevenly distributed among habitats, with the most widespread habitats (e.g., abyssopelagic and abyssal plains, areas beyond national jurisdiction – ABNJ) being the most undersampled. Similarly, the distribution and level of protection of marine protected areas varied between countries and deep-sea habitat types, with virtually no protection in ABNJ. This review provides a comprehensive summary for scientists and managers, including recommendations on how to fill remaining scientific knowledge gaps, expand deep-sea observations to ABNJ and poorly known habitats, increase capacity for deep-sea research, improve data sharing and better support conservation and management. These recommendations aim to prioritize future research and improve sustainable management in alignment with the goals of the United Nations Decade of Ocean Science for Sustainable Development.

The North Atlantic is an ocean basin with a diversity of deep-sea ecosystems. Here we provide a summary of the topography and oceanography of the North Atlantic including the Gulf of Mexico and Caribbean Sea, provide a brief overview of the history of scientific research therein, and review the current status of knowledge of each of 18 pelagic and benthic deep-sea ecosystems, with a particular focus on knowledge gaps. We analyse biodiversity data records across the North Atlantic and highlight spatial data gaps that could provide important foci for future expeditions. We note particular data gaps in EEZs of nations within and bordering the Caribbean Sea. Our data provide a baseline against which progress can be tracked into the future. We review human impacts caused by fishing, shipping, mineral extraction, introduction of substances, and climate change, and provide an overview of international, regional and national measures to protect ecosystems. We recommend that scientific research in the deep sea should focus on increasing knowledge of the distribution and the connectivity of key species and habitats, and increasing our understanding of the processes leading to the delivery of ecosystem services. These three pillars - distribution, connectivity, ecosystem function - will provide the knowledge required to implement conservation and management measures to ensure that any deep-sea development in the future is sustainable. Infrastructure and capacity are unevenly distributed and implementation of strategies that will lead to more equitable deep-sea science is required to ensure that essential science can be delivered.

To meet scientific, policy, and community goals, there is a critical need to strengthen research capacity, increase monitoring, and inform adaptation and mitigation policies to enhance resilience against ocean acidification (OA) and associated multi-stressors in the Caribbean. In 2023, an OA Needs Based Assessment survey of ocean professionals was conducted, engaging 59 participants from across the wider Caribbean to evaluate regional challenges and opportunities in OA research and monitoring. To understand differences in OA research capacity related to training and funding, we divide the respondents into four groups: those that have received 1) training and funding, 2) training only, 3) funding only, and 4) neither training nor funding. Results indicate regional strengths include awareness of local oceanic conditions, access to nearshore sites, and strong social support networks in ocean research. Regional barriers include limited technical capacity and funding to conduct oceanographic research and monitoring, and in particular, carbonate measurements. The four training and funding groups vary significantly, suggesting that access to training and funding are important factors to increasing the amount of access that respondents have to different types of equipment, the number of different types of measurements they conduct, the number of different habitats they research, and the amount of experience they have conducting OA research. This study also demonstrates the community-led efforts to address local OA challenges by presenting a case study on the formation of the Global Ocean Acidification Network (GOA-ON) OA Caribbean Hub that was founded by local leaders (co-authors of this study) who were inspired through the survey process and engagement that was conducted by co-authors. This study provides examples of avenues and challenges to build OA capacity for research and monitoring from the ground up within the wider Caribbean to advance towards global sustainability goals.

The Chapopote Knoll at 3200 m depth, in the southern Gulf of Mexico harbors highly diverse benthic habitats, including massive asphalt flows and surficial gas hydrates with gas seepage. Its associated benthic megafauna includes endemic cold-seep species and background species. This study describes the benthic habitat preferences, distribution patterns and diets of three crustacean species, the caridean shrimp Alvinocaris muricola and the galatheids Munidopsis geyeri and M. exuta. High-resolution imaging recorded eight habitats and helped depict their spatial distributions. A. muricola aggregates on Siboglinidae clusters and in gas seepage sites. M. geyeri and M. exuta are less selective and occur in almost all habitats. The carbon (δ13C) and nitrogen (δ15N) values of A. muricola show a nutritional preference of bacteria from mats and water column detritus retained among the Sibolindiae, whereas the two Munidopsis species have wider spectrum diets. Gut content analysis in all three species, validate the stable isotope values, food sources and confirm the secondary consumer’s trophic level. This study recognizes coexistence of A. muricola and the two Munidopsis species in the benthic habitats while using different resources. Compound specific isotope analyses of galatheid guts revealed females to have more 13C-depleted lipids (-35‰) compared to males (-28‰), calling for more detailed analyses to clarify this trophic segregation.

The intricate relationship between prokaryotic vitamin B12 (cobalamin) producers and metazoans in deep-sea ecosystems, particularly within ferromanganese crusts and polymetallic nodules, is critical for understanding oceanic biogeochemical cycling of cobalt. Microbial communities are key regulators of essential biogeochemical cycles, with cobalt serving as a vital component in the synthesis of cobalamin, a metallocofactor indispensable for numerous metabolic processes. We analyzed the significance of cobalamin biosynthetic pathways confined to prokaryotes and emphasized the ecological importance of auxotrophic organisms that rely on exogenous sources of vitamin B12. Additionally, we recognize recent research regarding the spatial distribution of dissolved cobalt and its consequential effects on cobalamin production and bioavailability, indicating the scarcity of cobalt and cobalamin in marine environments. We propose that cobalt-rich environments may foster unique interactions between prokaryotic and eukaryotic organisms, potentially altering the food web dynamics owing to the localized abundance of this element. By investigating the roles of cobalt and cobalamin in nutrient cycling and interspecies interactions, we outlined key criteria for future research on deep-sea microbial communities and their contributions to the cobalt biogeochemical cycle.

Robust impact assessments (IAs) for deep-sea fisheries are essential for safeguarding deep-sea ecosystems against the impacts of bottom fishing. In the high seas, United Nations Resolution commitments require States (independently or through Regional Fisheries Management Organisations (RFMOs)) to conduct IAs to evaluate if fishing is putting vulnerable marine ecosystems (VMEs) at risk. To enhance the efficacy of future IAs, this study evaluated nine IAs against the criteria in the FAO International Guidelines for the Management of Deep-Sea Fisheries in the High Seas. We find that in all IAs, the information required by the FAO Guidelines is either completely lacking or only partially addressed. The main shortcoming of the IAs was inadequate description of the ecosystems potentially affected by fishing. Additional shortcomings include incomplete description of the proposed fishing activities, lack of baseline data and risk assessments, and limited consideration of the indirect impacts of fishing. This study identifies several ways to strengthen the IA process; i) making IAs publicly available; ii) improved collection of baseline data and VME identification; iii) assessment of impacts on broader range of species associated with VMEs or potentially impacted by deep-sea fishing; iv) enhanced cooperation between RFMOs and more streamlined IA processes; v) comprehensive assessment of different types of impacts from fishing and climate change, and vii) improved consideration of uncertainty. Fully compliant IAs are a minimum requirement for the effective function of RFMOs, since they are the primary tool for preventing significant adverse impacts upon vulnerable marine ecosystems and the wider deep-sea environment.

The abyssal plains are vast areas without large scale relief that occupy much of the ocean floor. Although long considered relatively featureless, they are now known to display substantial biological heterogeneity across different spatial scales. Ecological research in these regions benefits increasingly from non-destructive visual sampling of epifaunal organisms with imaging technology. We analysed images from ultra-high-definition towed camera transects at depths of around 3500 m across three stations (100–130 km apart) in the Bering Sea, to ask whether the density and distribution of visible epifauna indicated any substantial heterogeneity. We identified 71 different megafaunal taxa, of which 24 occurred at only one station. Measurements of the two most abundant faunal elements, the holothurian Elpidia minutissima and two xenophyophores morphotypes (the more common identifiable as Syringammina limosa ), indicated significant differences in local densities and patchy aggregations that were strikingly dissimilar among stations. One station was dominated by xenophyophores, one was relatively depauperate in both target taxa as well as other identified megafauna, and the third station was dominated by Elpidia . This is an unexpected level of variation within comparable transects in a well-mixed oceanic basin, reinforcing the emerging view that abyssal habitats encompass biological heterogeneity at similar spatial scales to terrestrial continental realms.

Coastal communities of the Caribbean Sea and Gulf of Mexico have been affected by atypical influxes of pelagic macroalgae (Sargassum genus) since 2011, entailing ecological, economic and social impacts in need of characterization. We compiled and documented local ecological knowledge (LEK) and perceptions across diverse stakeholder groups from coastal communities in Mexico (Quintana Roo) (n=50 participants) and the United States (Florida) (n=36 participants) through on-site and online interviews and workshops undertaken from January to March of 2022, to understand how the knowledge of this phenomenon varies among communities and to characterize ecological and well-being impacts. Participants in Quintana Roo associated these influxes with both global phenomena (e.g., climate change) and local scale processes (e.g., currents/wind patterns) while Florida participants associated these events more with the latter. The communities in both regions perceived that the economy and the environment were the most impacted well-being categories. While influxes effects were mostly negative (80%) according to Quintana Roo participants (e.g., affected fisheries), Florida participants considered many positive effects of Sargassum (40%) on several well-being and ecological components (e.g., nursery habitat for marine species). In general, the perception of Sargassum as a problem was less pronounced in Florida, and these differences in perception are related to the magnitude of these influxes’ effect on the daily life of these communities. Overall, macroalgae management is still mainly focused on beach cleanup. Documenting LEK is important to delineate scientific research priorities and to provide decision makers with resources to develop efficient public policies and coastal management decisions.

This study collates and reviews the state of the art in the phenomenon of atypical pelagic Sargassum influxes in the coastline of the Mexican Caribbean, focusing on projects, studies and initiatives that have been conducted in the country for a decade. We integrated multisectoral and multidisciplinary knowledge and identified gaps and strengths in current knowledge. Initiatives and projects conducted in Mexico are numerous, diverse and valuable. However, interdisciplinary and transdisciplinary research and interinstitutional coordinated actions in the medium- and long-term are still lacking. Because of this, there is an imbalance of actions in different knowledge areas that prevents this phenomenon from being addressed in a comprehensive way. Furthermore, the funding opportunities for Sargassum research projects and other initiatives seem to respond to the events of massive influxes, without continuity or long-term planning. Attention is mainly focused on urban and touristic areas, so impacts to rural or uninhabited zones are unknown. This review represents a stepping-stone towards an integrated multisectoral effort to shift the perspective from Sargassum being a “national problem” to a “national resource,” considering and fully understanding the ecological importance of this macroalgae as a floating ecosystem and its potential as an economic resource once it massively arrives in Mexican coastal areas.

The history of colonization and dispersal of fauna among deep-sea chemosynthetic ecosystems remains enigmatic and poorly understood. The distribution of squat lobsters of the genus Munidopsis Whiteaves, 1874 can be influenced by the rich organic matter and associated organism communities of chemosynthetic ecosystems. The present work analyzed the molecular relationships and morphology of individuals from different populations of Munidopsis exuta Macpherson & Segonzac, 2005 and M. geyeri Pequegnat & Pequegnat, 1970 in such ecosystems along the Atlantic Equatorial Belt, including the Chapopote Knoll, in the southern Gulf of Mexico. Munidopsis geyeri is re-described based on the present findings and reference to the literature. This analysis documented the genetic distances, as well as range of variation in the diagnostic characters that support the separation of M. exuta and M. geyeri. Our results confirm that the two species coexist in seep ecosystems and have an amphi-Atlantic distribution.

Citation: Molony BW, Ford AT, Sequeira AMM, Borja A, Zivian AM, Robinson C, Lønborg C, Escobar-Briones EG, Di Lorenzo E, Andersen JH, Müller MN, Devlin MJ, Failler P, Villasante S, Libralato S and Fortibuoni T (2022) Editorial: Sustainable Development Goal 14 Life Below Water: Towards a Sustainable Ocean. Front. Mar. Sci. 8:829610. doi: 10.3389/fmars.2021.829610 Editorial: Sustainable Development Goal 14 Life Below Water: Towards a Sustainable Ocean

The frequency and intensity of ocean related hazards and its overlapping and cascading effects are escalating with devastating and unsustainable impact on life and livelihoods in the Western Tropical Atlantic. From the Bahamas to Brazil, from Mexico and Central America to the Lesser Antilles, coastal and maritime communities have been impacted by hurricanes, storm surges, earthquakes, sargassum and oil spills along with other climate, weather and human induced events. In addition to the coastal impacts, ocean hazards also have affected the safe and timely delivery of goods and services in a region that is extremely dependent on maritime transport. COVID-19 has compounded even more the situation of millions of people in the region and put extreme pressure on government officials, including disaster management and health professionals as well as the maritime industry. Climate change will only exacerbate the situation. The UN Decade of Ocean Science for Sustainable Development (2021-2030) presents itself as a once-in-a-lifetime opportunity to stop the cycle of disasters with "A Safe Ocean" as one of its seven major societal outcomes. The Western Tropical Atlantic Safe Ocean Working Group was established to co-design and co-deliver with local, national and regional partners transformative strategies that will facilitate and inspire actions to reduce and mitigate the ocean related threats while creating resilient and safer coastal communities and maritime activities. At the core is an Integrated Multi Hazard Ocean Data and Forecast System. Its services would be linked to education, outreach, readiness and communication that empowers and recognizes national and local policy decisions and includes individual response for the protection of life and livelihoods. This effort should support relevant science and strengthen capacity leaving no one or country behind.

Although there is no universally accepted definition of ‘traditional knowledge’ in international law and environmental governance, we refer here to TK as the knowledge, innovations and practices developed from experience gained over the centuries and adapted to the local culture and environment. It may be collectively owned and passed down through millennia in the form of stories, songs, chants, folklore, proverbs, dances, paintings, cosmologies, cultural values, beliefs, rituals, community laws, local language, and practices. TK helps transmit identity, relationships among peoples, with ancestors and with the environment. For the deep sea, TK is manifested in the preservation and perpetuation of connections between people, species, processes, ecosystems and seascapes. This includes an explicit approach for effective environmental management.

Growing human activity in areas beyond national jurisdiction (ABNJ) is driving increasing impacts on the biodiversity of this vast area of the ocean. As a result, the United Nations General Assembly committed to convening a series of intergovernmental conferences (IGCs) to develop an international legally-binding instrument (ILBI) for the conservation and sustainable use of marine biological diversity of ABNJ [the biodiversity beyond national jurisdiction (BBNJ) agreement] under the United Nations Convention on the Law of the Sea. The BBNJ agreement includes consideration of marine genetic resources (MGR) in ABNJ, including how to share benefits and promote marine scientific research whilst building capacity of developing states in science and technology. Three IGCs have been completed to date with the fourth delayed by the Covid pandemic. This delay has allowed a series of informal dialogues to take place between state parties, which have highlighted a number of areas related to MGR and benefit sharing that require technical guidance from ocean experts. These include: guiding principles on the access and use of MGR from ABNJ; the sharing of knowledge arising from research on MGR in ABNJ; and capacity building and technology transfer for developing states. In this paper, we explain what MGR are, the methods required to collect, study and archive them, including data arising from scientific investigation. We also explore the practical requirements of access by developing countries to scientific cruises, including the sharing of data, as well as participation in research and development on shore whilst promoting rather than hindering marine scientific research. We outline existing infrastructure and shared resources that facilitate access, research, development, and benefit sharing of MGR from ABNJ; and discuss existing gaps. We examine international capacity development and technology transfer schemes that might facilitate or complement non-monetary benefit sharing activities. We end the paper by highlighting what the ILBI can achieve in terms of access, utilization, and benefit sharing of MGR and how we might future-proof the BBNJ Agreement with respect to developments in science and technology.

Abstract Marine Life 2030 is a program to establish the globally coordinated system to deliver actionable, transdisciplinary knowledge of ocean life to those who need it, promoting human well-being, sustainable development, and ocean conservation (Figure 1). The diversity of marine habitats and species is fundamental for human survival. Biodiversity provides opportunities for multiple fisheries, the tourism industry, and harbor medicines and materials. The Marine Biodiversity Observation Network (MBON) is the platform to build the community of practice to implement Marine Life 2030. MBON fosters collaborations to coordinate collection, sharing, and application of biodiversity information. Benefits of joining MBON include expanded capacity to address research goals, leveraging resources and best practices; linking natural and social sciences to answer policy questions; engaging diverse and early-career researchers; and addressing issues of concern to humanity.