Annals of the New York Academy of SciencesVolume 174, Issue 1 p. 177-188 THE EQUILIBRIUM OF CARBON MONOXIDE WITH HUMAN HEMOGLOBIN IN WHOLE BLOOD F. J. W. Roughton F. R. S., F. J. W. Roughton F. R. S. Laboratory of Biophysical Chemistry and Colloid Science, University of Cambridge Cambridge, EnglandSearch for more papers by this author F. J. W. Roughton F. R. S., F. J. W. Roughton F. R. S. Laboratory of Biophysical Chemistry and Colloid Science, University of Cambridge Cambridge, EnglandSearch for more papers by this author First published: October 1970 https://doi.org/10.1111/j.1749-6632.1970.tb49784.xCitations: 43AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Citing Literature Volume174, Issue1Biological Effects of Carbon MonoxideOctober 1970Pages 177-188 RelatedInformation

Research Article| May 01 1970 Some recent work on the interactions of oxygen, carbon dioxide and haemoglobin F J W Roughton F J W Roughton Search for other works by this author on: This Site PubMed Google Scholar Biochem J (1970) 117 (5): 801–812. https://doi.org/10.1042/bj1170801b Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation F J W Roughton; Some recent work on the interactions of oxygen, carbon dioxide and haemoglobin. Biochem J 1 May 1970; 117 (5): 801–812. doi: https://doi.org/10.1042/bj1170801b Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Journal Search Advanced Search © 1970 The Biochemical Society1970 Article PDF first page preview Close Modal You do not currently have access to this content.

Abstract : A new method of investigation, based on the use of rapidly responding pCO2 electrodes, was found suitable for the study of the kinetics of carbamino reaction, the extent of CO2 combination being obtained in 5 msec after the beginning of the reaction. The method is adequate for a full kinetic analysis of this rapid reaction. The method has been applied to the study of the reaction between CO2 and amino groups of haemoglobin molecule. (Author)

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The rapid reaction method introduced in 1923 to study the reaction of oxygen with haemoglobin is discussed. Results on haemoglobin solutions are compared with those on suspensions of red blood corpuscles and the rate‐limiting effects of the various processes involved in the oxygenation of blood in the lungs determined.

The Journal of PhysiologyVolume 146, Issue 3 p. 583-593 ArticleFree Access The time course of the effects of carbon monoxide on visual thresholds M. H. Halperin, M. H. HalperinSearch for more papers by this authorR. A. McFarland, R. A. McFarlandSearch for more papers by this authorJ. I. Niven, J. I. NivenSearch for more papers by this authorF. J. W. Roughton, F. J. W. RoughtonSearch for more papers by this author M. H. Halperin, M. H. HalperinSearch for more papers by this authorR. A. McFarland, R. A. McFarlandSearch for more papers by this authorJ. I. Niven, J. I. NivenSearch for more papers by this authorF. J. W. Roughton, F. J. W. RoughtonSearch for more papers by this author First published: 11 June 1959 https://doi.org/10.1113/jphysiol.1959.sp006213Citations: 65AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Citing Literature Volume146, Issue3June 11, 1959Pages 583-593 RelatedInformation

Annals of the New York Academy of SciencesVolume 75, Issue 1 p. 156-166 PROBLEMS CONCERNING THE KINETICS OF THE REACTIONS OF OXYGEN, CARBON MONOXIDE, AND CARBON DIOXIDE IN THE INTACT RED CELL F. J. W. Roughton, F. J. W. Roughton The Department of Colloid Science, Cambridge University, Cambridge, EnglandSearch for more papers by this authorJ. C. Rupp, J. C. Rupp The Department of Colloid Science, Cambridge University, Cambridge, EnglandSearch for more papers by this author F. J. W. Roughton, F. J. W. Roughton The Department of Colloid Science, Cambridge University, Cambridge, EnglandSearch for more papers by this authorJ. C. Rupp, J. C. Rupp The Department of Colloid Science, Cambridge University, Cambridge, EnglandSearch for more papers by this author First published: October 1958 https://doi.org/10.1111/j.1749-6632.1958.tb36861.xCitations: 12AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Citing Literature Volume75, Issue1Enzymes in BloodOctober 1958Pages 156-166 RelatedInformation

The over-all velocity constants, kc' and lc' (in mm−1 x sec.−1), for the combination of O2 and CO, respectively, with reduced hemoglobin in 1:80 human red cell suspensions at 37°C have been measured in a Hartridge-Roughton continuous flow apparatus. Relative pigment concentration in the flowing cell suspension was estimated from the difference in light transmission at two different colors in order to reduce the influence of light scattering. The average value of kc' was 92; that for lc' was 79. Values for corresponding velocity constants for the uptake of O2 and CO by a membrane free layer of concentrated hemoglobin solution, comparable in dimensions to the erythrocyte, have been calculated from previously reported values for the rates of reaction and diffusion in hemoglobin solutions, and are, respectively, 3.6 and 2.3 times greater than kc' and lc'. We assume that this difference reflects the additional resistance to gas diffusion into the erythrocyte imposed by the cell membrane, and on this basis we have calculated values for the ratio of the permeability of the red cell membrane to the permeability of the red cell interior (λ). For human red cells at 37°C, the value of λ is about 1.5, both for O2 and for CO penetration. Submitted on February 15, 1957

An equation, i/Dm + i/θVc = i/Dl, has been derived which relates the measured pulmonary diffusing capacity (Dl), the true diffusing capacity of the pulmonary membrane (Dm), the rate of uptake of CO by the red cells per mm Hg CO tension (θ) and the blood volume of the pulmonary capillary bed (Vc). By making measurements of Dl at different alveolar O2 tensions, thereby causing to vary, this equation can be solved graphically for Dm and Vc, which are assumed to be independent of O2 tension. Calculations of Dm and Vc were made utilizing a) values of θ previously obtained from the in vitro rates of CO uptake of suspensions of human red cells at 37°C and b) values of Dl in normal resting subjects at alveolar O2 tensions from about 100 mm Hg to over 600 mm Hg measured by both steady state and breath holding CO techniques. Dm is about twice the value of Dl measured in subjects breathing air at sea level. Vc is about 75 ml in approximate agreement with the previously reported estimate of Roughton. Similar results were obtained using values of Dl at different alveolar O2 tensions reported in the literature. This means that, in determining the rate of CO absorption in the lungs, the resistance of the red cell to the uptake of CO is of the same order of importance as the resistance of the pulmonary membrane to the diffusion of gas across it. Arguments are advanced to show that red cell resistance is of at least equal importance in the case of O2 uptake. Submitted on February 15, 1957

The apparent pulmonary diffusing capacity for CO (Dl) has been measured at alveolar O2 tensions from 40 to more than 600 mm Hg. Two methods were used: a) in six healthy subjects a steady state method in which the end-expiratory tension of a continuous record of respired CO concentration was considered 'alveolar' and b) in seven healthy subjects a breath holding technique. Dl, measured by either method, decreased with increasing alveolar O2 tension in all subjects, varying as much as fivefold over the entire range. All estimates of Dl were corrected for the presence of COHb in the mixed venous blood. Dl (steady state; breathing air) was on the average 0.85 of Dl (breath holding; breathing air). Submitted on February 15, 1957

The rate at which CO replaces O2 in combination with human hemoglobin in solution and in red cell suspensions at 37°C was determined in vitro on the blood of six normal subjects at O2 tensions from 100 to over 600 mm Hg, by means of modifications of the Hartridge-Roughton rapid reaction velocity apparatus, using either a reversion spectroscope or a two-color photocolorimetric method. At low ratios of [CO] to [O2] (i.e. <0.1), the rate of the reaction in Hb solution conforms theoretically to the equation d[COHb]/dt = m∞'[CO][O2Hb]/[O2], where m∞' is a true velocity constant. Experimentally, it was not possible to use ratios of [CO] to [O2] <0.2, and the results for Hb solutions were interpreted in terms of the equation d[COHb]/dt = m∞'[CO][O2Hb[/]O2], where m' is an apparent velocity constant. The measured values of m' in Hb solution ranged from 6.8 to 22.4 sec.−1 as O2 tension rose, the average value of m∞' deduced therefrom being 18.8. The apparent velocity constant for cell suspensions, mc', is less than m' owing to the limiting effects of diffusion. The ratio of mc' to m' rose from 0.46 at an O2 tension of 100 mm Hg to 0.68 at an O2 tension of 571 mm Hg.λ, the ratio of the permeability of the red cell membrane to that of the red cell interior, was calculated from these data to average 1.53. The values of mc' calculated from the in vitro experimental results cannot be used directly for calculations related to in vivo pulmonary diffusing capacity experiments, because the CO tension is over 40-fold greater in the former. Corrections for this have been made and proper values of mc' obtained for use in the calculation of true pulmonary diffusing capacity and pulmonary capillary blood volume. Submitted on February 15, 1957